应用两种稀释液冷冻驴精液试验报告

本试验选用两种冷冻稀释液,通过离心去除精清的方法对驴精液进行了冷冻。结果发现在试验条件相同的情况下,用配方2冷冻稀释液冷冻精液的效果好于配方1,其平衡后活率(0.700±0.038)极显著高于配方1的平衡后活率(0.605±0.040)(P<0.01);经过冷冻后,其冻后活率(0.460±0.066)也极显著高于配方1的冻后活率(0.325±0.064)(P<0.01);且其精子顶体完整率(0.800±0.031)也极显著高于配方1(0.736±0.024)(P<0.01)。这表明配方2是1个冷冻驴精液的较好配方。     

稀释液中加入低密度脂蛋白对牛冷冻精液的影响

研究在传统稀释液的基础上,探索分析鸽蛋中提取的低密度脂蛋白(LDL)对牛精液的冷冻效果,以冻后精子活力、活率、顶体完整性、质膜完整性等作为质量评定指标,同时分析比较低密度脂蛋白、法国OptidylTM稀释液冻后精子参数,为牛冻精的基础研究提供理论依据,对生产中进一步提高牛冻精质量具有重要的指导作用. 1材料与方法 1.1LDL的提取     

废旧电池内容物直接填埋和其稀释液对香根草生长胁迫与光合生理生态的研究

通过盆栽试验,研究了废旧电池在2种处理方式不同浓度下胁迫对香根草的细胞膜透性、脯氨酸(Pro)含量、叶绿素(Chl)含量、光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)等的影响。结果表明,随稀释液浓度、内含物量增加,香根草叶片生长速率、Chl含量、Pn、Tr和Gs虽呈下降趋势,而Pro含量有所升高,膜透性增大,但不同稀释液和内容物填埋处理对香根草的胁迫毒害作用差异不明显,在稀释液浓度为14.44%或以下和内容物占土壤2.70%或以下时,香根草能生长,在稀释液浓度为20.20%或以上和内容物占土壤4.00%或以上时,香根草均死亡。     

Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet

The aim was to explore the effects of different kinds of dilution and thawing devices on the N,N-dimethylformamide (DMA) pellet frozen semen of Black Silkies. Firstly,the motility and fertility of the frozen semen thawed by different dilutions were compared;Then,the motility of the frozen semen was compared when the pellets were thawed using different tube and different number.Finally,the motility and fertility of the sperm thawed by three kinds of thawing devices (thermostat water bath,hotfunnel and hotplate ) were tested. The results showed that:①There was similar order of the sperm motility and fertility in the different dilution groups (LR > F > B > L),and there was significant difference among those groups (P < 0.05).②The motility was the best when the frozen semen was thawed with large thin-wall glass tube at 60℃.③The best temperature range of the 3 devices was different. The highest motility for thermostat water bath was 50 to 60℃ (0.51 to 0.59),and thermostat hotfunnel was 40 to 45℃ (0.42 to 0.46),while thermostat hotplate was 50 to 55℃ (0.61 to 0.63).There was no significant difference of the motility in the optimum temperature range for each device (P > 0.05).④The fertility of the different devices in their best thawing temperature was 26.91% (55℃,thermostat hotplate),23.08% (60℃, thermostat water bath), 20.93% (40℃, thermostat funnel),respectively,and there was no significant differences among those groups (P > 0.05).Therefore,the efficiency thawing condition for the Black Silkies frozen semen was the LR diluent,DMA cryoprotectant,pellet freezing,thawed in the thermostat hotplate at 54.9℃.     

平板凝集反应预防新生骡驹溶血病的临床应用

新生骡驹溶血病是由公驴和母马杂交所产幼驹,吮食高效价抗体初乳后引发的一种急性、免疫性、溶血性疾病.临床上以贫血、黄疸、血红蛋白尿为主要特征.新生骡驹溶血病时有发生,给饲养户造成一定的经济损失.发病后目前多以停喂母马初乳、输血、补充造血物质为主的治疗方法,但治愈率不高.     

德国牧羊犬精液冷冻保存的研究

以德国牧羊犬为实验动物,用两种不同的冷冻方法和剂型对犬的精液进行冷冻,以冻精解冻后的活率作为评定指标,提高犬精液利用效率和扩大犬精液的使用范围。采集4条公犬的精液40次,分别在四种稀释液中稀释,冷冻成两种剂型,根据冻后活率比较优劣。结果表明,稀释液2颗粒冻精的冻后活率显著高于稀释液1、稀释液3和稀释液4(P0.05);对犬的精液进行炸熏法和程序冷冻法冷冻,炸熏法冷冻的精液冻后活率均显著高于程序冷冻法冷冻的精液(P0.05)。     

为什么不能用凉开水稀释疫苗

凉开水复杂的理化性质决定了它不适合作为疫苗稀释液．因为弱毒苗的核心物质是它所含的弱毒细菌或弱毒病毒．它们都是活的微生物。     

黑熊精液的保存研究

对35只4～13岁的健康雄性黑熊进行了电刺激采精161次，并对精液进行了冷冻保存。稀释后低温保存48h，平均精子活力可达到0．3以上。用含甘油的稀释液稀释后，最佳平衡时间为2h。     

种公鸡精液稀释液的研究

种公鸡精液稀释与人工授精技术相结合,可以提高种公鸡的利用率,降低饲养成本,增加经济效益。就影响鸡精液稀释保存效果的相关因素,如稀释液物质组成、pH值、渗透压、稀释比例、稀释操作、输精频率等作一综述。     

公猪精液长效稀释液配方研究

利用国内外猪精液常温保存的研究成果,设计了4个公猪精液稀释液配方(T1、T2、T3、T4),以目前四川农村猪人工授精中常用的EDTA液配方为对照,在室温条件下(16～28℃)通过逐日镜检的方法检查精子活力,每个配方5次重复,筛选出了保存期达5天、精子活力0.5级以上的配方2个(T2、T3);保存期达6天、精子活力0.5级以上的配方1个(T4).使用T4配方保存6天的稀释精液输精母猪68头,经超声波妊娠诊断仪检测母猪受胎率达86.8%.对4个精液稀释液配方在溶解后90min内的pH值变化进行了研究,各稀释液在溶解后的60min内pH值出现明显波动,之后达到平衡,表明稀释液宜在使用前1h配制好.