细胞自噬与猪流行性腹泻病毒相互作用的研究进展

猪流行性腹泻病毒（PEDV）是冠状病毒科甲型冠状病毒属的一种有囊膜的单股正链RNA病毒，对全世界养猪业造成了巨大的损失。PEDV与宿主之间的致病机制和免疫调节相互作用仍不清楚。有研究表明冠状病毒可以诱导细胞发生自噬，并且与病毒的复制有着密切的联系，病毒与细胞自噬之间的关系十分复杂，虽然病毒感染与细胞自噬之间的关系研究比较广泛，但细胞自噬在PEDV感染中的作用报道较少。研究PEDV与细胞自噬的相互作用可以加速对PEDV感染入侵机制的理解，为未来研究病毒治疗和抗病毒药物提供新的思路。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

冬春季猪流行性腹泻病的防制措施

<正>猪流行性腹泻是由病毒引起的仔猪和育肥猪的一种急性肠道传染病。本病与传染性胃肠炎很相似,在我国多发生在每年12月份至翌年1~2月,夏季也有发病的报道。可发生于任何年龄的猪,年龄越小,症状越重,死亡率高。一股哺乳仔猪、断奶仔猪和育肥猪感染发病率100%,成年母猪为20%左右。哺乳仔猪受害最严重,病死率平均50%,常在一月时间内传遍整个猪场。(一)流行特点(1)只感染猪,各种年龄、品种均可感染发病。(2)主要发生于冬季12月至次年2月的寒冬季节。(3)哺乳仔猪、断奶仔猪和育肥猪     

猪流行性腹泻病毒分离鉴定和灭活疫苗免疫保护效果探讨

猪流行性腹泻病毒是诱发猪流行性腹泻的关键因素,而猪流行性腹泻容易造成养殖猪,尤其是猪仔的死亡,进而给养猪业带来严重的经济损失.基于此,本文首先阐述了猪流行性腹泻的流行病学,紧接着分析了猪流行性腹泻的临床症状与病理变化,然后分析了猪流行性腹泻病毒的分离鉴定,并进行灭活免疫实验探究,希望能够为今后相关问题的研究提供一定的参考依据.     

我国猪流行性腹泻病毒分子遗传变异研究浅析

猪流行性腹泻是由猪流行性腹泻病毒引起猪的一种急性、高度传播的肠道疾病。近年来PEDV基因变异毒株不断出现，造成了猪流行性腹泻防疫失败现象不断增多，导致其流行趋势增强。阐述了近年来国内有关PEDV的S1、M和ORF3基因的研究进展，从分子生物学角度对PEDV进行遗传进化分析总结，为该病的防控以及流行病学研究提供参考。     

Identification and Molecular Characteristics Analysis of Porcine Epidemic Diarrhea Virus Variants

To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains.     

仔猪腹泻原因分析与防治措施

仔猪腹泻是仔猪养育阶段最常见也是最棘手的一类病症,据不完全统计,在猪场中该类病症占到仔猪发病的70,由其导致的死亡占到仔猪死亡总数的50,给养猪业造成了重大经济损失。现     

猪传染性胃肠炎及流行性腹泻的防治

猪传染性胃肠炎及流行性腹泻都是因冠状病毒所引发的一种高度接触的急性肠道传染病，其主要表现特征是腹泻、呕吐以及脱水。最近几年猪传染性胃肠炎及流行性腹泻在各个养猪场出现的越来越频繁，并且有混合感染的趋势。     

牛流行性流产的临床诊断及防治

牛流行性流产是一种传染性疾病,牛感染病原体后,主要症状为流行性或地方性流产。牛流行性流产不仅会对生产期母牛造成器官损伤,同时也会大范围传染其他牛群,造成重大损失。本论文将从牛流行性流产的流行病学出发,介绍牛流行性流产相关临床诊断指标,并提出相关预防与治疗措施。     

生猪报告看涨带来的影响

<正>上周,6月的生猪报告看涨,瘦肉猪期货因此飙升。10月、12月及2月的期货分别上涨了6美元/磅、7美元/磅和8美元/磅。仅仅4 d时间,芝加哥商品交易所的秋季和初冬上市的生猪上涨了将近15美元/头。生猪数量减少及强劲的猪肉需求带来了北美生猪产业历史上的最高利润。在上周的同样四天里,九月和十二月的玉米下跌了33美分/蒲式耳,而十一月的大豆下跌了94美分/蒲式耳。上涨的生猪价格及玉米和大豆价格的下跌可能会使秋季和初冬期间上