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In order to detect Enterococcus faecalis endocarditis antigen (EfaA) of bear that was used to disclose the infected Northeast Black bear immediately, a pair of PCR primers was synthesized by EfaA gene of Enterococcus faecalis that was recorded in GenBank (accession number:U03756.1) and the target fragment was 689 bp. The PCR product was cloned by pMD19-T vector, then was transferred to Escherichia coli DH5α competent cells and the positive clones were filtered. Recombinant plasmid (pMD19-T-EfaA) was extracted. Identification of PCR and digestion, sequencing, the structure prediction of proteins were operated. The results showed that EfaA gene was cloned successfully, and the gene homology with ATCC 29212 was 100.0%. pMD19-T-EfaA was constructed successfully, structure of proteins was predicted. This study provided theoretical basis for diagnostic methods of Enterococcus faecalis and laid basis for prevention and control of Northeast Black bear disease. 相似文献
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猪链球菌病是由几种主要链球菌引起的,常表现为猪急性败血症、脑炎、局灶性淋巴结化脓、慢性关节炎及心内膜炎。而仔猪主要是急性败血症及脑炎,部分淋巴结化脓。 相似文献
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猪链球菌病的诊断及综合防制 总被引:1,自引:0,他引:1
猪链球菌病是一种人畜共患的急性、热性传染病,由C、D、E及L群链球菌引起的猪的多种链球菌病的总称。各种年龄的猪都可感染发病,以新生仔猪、哺乳仔猪的发病率和病死率高。猪链球菌感染不仅可致猪败血症肺炎、脑膜炎、关节炎及心内膜炎,而且可感染特定人群发病,并可导致死亡。 相似文献
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猪链球菌病的实验室诊断 总被引:1,自引:0,他引:1
猪链球菌病是由多个血清群的链球菌引起的不同临诊类型的猪传染病总称,能引起猪的败血症、脑膜炎、关节炎、心内膜炎及组织化脓等,常见的有败血性链球菌病和淋巴结脓肿两种类型。该病是世界各地的常见病,在我国也时有流行,发病率有上升趋势,严重阻碍了我国养猪业的持续发展。近年来,由于周边疫区商品仔猪大量贩卖到我区引发了许多猪场以发热、关节肿胀、败血症和神经症状为特征的急性传染病,通过实验室检验确诊为猪链球菌病。 相似文献
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为检测熊源粪肠球菌心内膜炎抗原(EfaA)基因,用于快速检测东北黑熊粪肠球菌感染病例,本试验根据GenBank登录的粪肠球菌EfaA基因序列(登录号:U03756.1)合成了1对PCR引物,通过PCR法扩增合成长度为689 bp的目的片段。将PCR产物克隆于pMD19-T载体,之后将其转入大肠杆菌DH5α感受态细胞筛选阳性克隆。提取扩增后的重组质粒pMD19-T-EfaA并进行PCR、酶切鉴定、序列测定及蛋白质结构预测。结果显示,本试验成功克隆出熊源粪肠球菌EfaA基因,与GenBank登录的ATCC 29212菌株同源性为100.0%。本试验成功构建了重组克隆载体pMD19-T-EfaA,并对测序结果进行了蛋白质结构的预测,为进一步建立黑熊粪肠球菌病的诊断方法提供了理论依据,同时也为黑熊疾病的防制奠定了基础。 相似文献