A Potential Screening System for Identifying Sorghum Ecotypes with Increased Lysine in the Seeds

Classical “high-lysine” sorghum lines are characterized by smaller seeds than average, due to a decrease in prolamin synthesis and a subsequent decrease in yield. To exploit the natural variation in lysine content and to identify ecotypes with a seed lysine content higher than average, characterized by plump seeds, a method was developed based on root-growth inhibition of seeds growing on a medium containing aminoethylcysteine (AEC), a lysine analogue. By using a collection of sorghum mutants and ecotypes a correlation coefficient of 0.926 between root length and lysine content was established. This method, which uses the root length of plants growing on aec to indicate which lines have a potential elevated lysine content, can be applied for the screening of sorghum germplasm. Since this is a non-destructive method it can also be used at the individual seed level, for example for screening progenies of regenerated plants from in vitro culture to exploit the somaclonal variation.     

Re-defining tillage erosion: quantifying intensity–direction relationships for complex terrain

Abstract. Current tillage erosion models account for the influence of tillage direction in the magnitude of the soil (tillage) transport coefficient. It is argued here that this is counter-intuitive and causes significant problems in modelling tillage erosion in areas of complex terrain. This article examines whether a re-modelling of tillage erosion is possible that separates tillage direction (an interaction with the landform) from the soil transport coefficient (a measure of tillage intensity representing the combination of implement erosivity and soil erodibility). Experimental data for mouldboard ploughing upslope, downslope and cross-slope at Coombe Barton Farm, Devon are examined. Integration of data for all directions into a single relationship, which relates translocation in the direction of tillage to slope in the direction of tillage and translocation perpendicular to tillage to slope perpendicular to tillage, is not possible using previously published methods of analysis. However, when total translocation distance is regressed against the tangent of the slope at 45° to the tillage direction (bisecting the tillage direction and the direction of overturning) it is found that a single relationship can be used to describe tillage in all three directions. Therefore, this relationship is used to determine a single value of the soil transport coefficient ( k _fTa) for constant soil and implement conditions but different tillage directions. This redefinition of tillage is important both for true estimation of tillage erosion severity, the adirectional coefficient being 40% larger than the directional coefficient, and for modelling of tillage erosion in complex terrain. These improvements are vital when tillage erosion simulation is used to direct soil conservation strategies.     

杂交条纹鲈苗种小水泥池培育技术试验

用地上露天小水泥池8个．进行杂交条纹鲈苗种培育试验．放养313龄水花30000尾．通过天然饵料、藻粉投喂以及适当水质管理措施．经32天培育，共培育3．5～4．6cm体长的鱼种4336尾．最高成活率为19．1％，最低为6％，平均为14．5％。     

Historical range of variability in eastern Cascades forests,Washington, USA

The historical range of variability (HRV) has been suggested as a coarse filter approach to maintain ecosystem sustainability and resiliency. The historical range of variability in forest age structure for the central eastern Cascade Range in Washington State, USA was developed from historical fire return intervals and the manner in which fire acted as both cyclic and stochastic processes. The proportions of seven forest structural stages calculated through these processes were applied to the area of each forest series within the central eastern Cascades landscape. Early successional forest stages were more common in high elevation forest than low elevation forest. The historical proportion of old growth and late successional forest varied from 38 to 63 percent of the forested landscape. These process-based estimates are consistent with those developed from forest structural information. HRV is a valuable planning tool for ecosystem conservation purposes, but must be applied to real landscapes with consideration of both temporal and spatial scale. This revised version was published online in July 2006 with corrections to the Cover Date.     

Pathogenicity and Virulence of the Two Dutch VCGs of Verticillium dahliae to Woody Ornamentals

Two experiments were performed in two consecutive years to test whether isolates of different vegetative compatibility groups (VCGs) differ in their ability to cause disease in woody ornamentals, to study the host specificity of the isolates and to get an insight into disease development in woody hosts. A range of woody ornamental plant species, including Acer campestre, Acer platanoides, Acer pseudoplatanus, Catalpa bignonioides, Cotinus coggygria, Robinia pseudoacacia, Rosa canina, Syringa vulgaris and Tilia cordata, were root-dip inoculated with six isolates of Verticillium dahliae, belonging to the two VCGs that occur in the Netherlands (VCG NL-1 and VCG NL-2). Isolates belonging to each VCG caused severe symptoms of verticillium wilt in most plant species tested. Disease progress differed between plant species, but was generally the same for the two VCGs. No overall differences in virulence were observed between the two VCGs for external wilt symptoms, number of dead plants, or shoot length. No significant VCG × plant species interactions were present for these characteristics. However, isolates of VCG NL-1 caused more vascular discolouration than did isolates of VCG NL-2. Isolates within VCGs often differed considerably in their virulence to certain hosts, as shown by highly significant isolate × plant species interactions. Isolates were more virulent on their original host. These findings imply that VCG identification does not contribute to disease prediction for a range of woody hosts.     

Spatial dependence and the relationship of soil organic carbon and soil moisture in the Luquillo Experimental Forest,Puerto Rico

We used geo-spatial statistical techniques to examine the spatial variation and relationship of soil organic carbon (SOC) and soil moisture (SM) in the Luquillo Experimental Forest (LEF), Puerto Rico, in order to test the hypothesis that mountainous terrain introduces spatial autocorrelation and crosscorrelation in ecosystem and soil properties. Soil samples (n = 100) were collected from the LEF in the summer of 1998 and analyzed for SOC, SM, and bulk density (BD). A global positioning system was used to georeference the location of each sampling site. At each site, elevation, slope and aspect were recorded. We calculated the isotropic and anisotropic semivariograms of soil and topographic properties, as well as the cross-variograms between SOC and SM, and between SOC and elevation. Then we used four models (random, linear, spherical and wave/hole) to test the semi-variances of SOC, SM, BD, elevation, slope and aspect for spatial dependence. Our results indicate that all the studied properties except slope angle exhibit spatial dependence within the scale of sampling (200 – 1000 m sampling interval). The spatially structured variance (the variance due to the location of sampling sites) accounted for a large proportion of the sample variance for elevation (99%), BD (90%), SOC (68%), aspect (56%) and SM (44%). The ranges of spatial dependence (the distances within which parameters are spatially dependent) for aspect, SOC, elevation, SM, and BD were 9810 m, 3070 m, 1120 m, 930 m and 430 m, respectively. Cross correlograms indicate that SOC varies closely with elevation and SM depending on the distances between samples. The correlation can shift from positive to negative as the separation distance increases. Larger ranges of spatial dependence of SOC, aspect and elevation indicate that the distribution of SOC in the LEF is determined by a combination of biotic (e.g., litterfall) and abiotic factors (e.g., microclimate and topographic features) related to elevation and aspect. This demonstrates the importance of both elevation and topographic gradients in controlling climate, vegetation distribution and soil properties as well as the associated biogeochemical processes in the LEF.This revised version was published online in May 2005 with corrections to the Cover Date.     

Comparison of Crinipellis perniciosa isolates from Brazil by ERIC repetitive element sequence-based PCR genomic fingerprinting

Fifty isolates of Crinipellis perniciosa originating from Theobroma cacao , Heteropterys acutifolia and Solanum lycocarpum , from six states within Brazil, were characterized through ERIC-PCR, representing the first application of this method for molecular characterization within C. perniciosa . Phenetic analysis of banding patterns revealed a separation of isolates on the basis of host of origin, with T. cacao -derived isolates showing only a 0·2 similarity level to a cluster comprising the isolates from H. acutifolia and S. lycocarpum . Considerable intraspecific variability was observed within C. perniciosa isolates from T. cacao , with distinct groups observed correlating with geographical origin. Given that a number of isolates from T. cacao from the Amazon region grouped with isolates from Bahia state, this work discusses the possibility that current C. perniciosa populations pathogenic on T. cacao in Bahia originated from the Amazon region, rather than from alternative host plants.     

雨洪利用工程在农村建设中的应用

随着经济的快速发展,水资源短缺矛盾日益突出,通过对国内外雨洪利用情况进行介绍,着重阐述了坑塘雨洪利用工程及沟道雨洪利用工程,并以房山雨洪利用工程为例,详细介绍了2种工程的设计方法、内容和工艺。     

砖红壤香蕉园土壤养分空间变异研究

运用统计学和ArcGIS软件的地统计分析模块对海南砖红壤香蕉园59个土壤采样点两个取样土层（0～20 cm和20～40 cm）养分进行空间变异性研究。结果表明：香蕉园土壤各养分变异系数均为中等变异。0～20 cm土层土壤各养分含量大于20～40 cm土层。0～20 cm和20～40 cm土层中的碱解氮、交换性钙和交换性镁及0～20 cm土层的有机质均符合高斯模型，0～20 cm土层速效磷与20～40 cm土层速效钾符合球状模型，而20～40 cm土层速效磷与0～20 cm土层速效钾符合圆形模型，20～40 cm土层有机质符合指数模型。各养分的变程范围为109.217～279.796 m，有机质具有强烈的空间相关性。0～20 cm土层速效钾的块金效应值小于20～40 cm土层，0～20 cm土层碱解氮、速效磷、交换性钙、交换性镁高于20～40 cm土层。有机质、20～40 cm土层速效磷、0～20 cm土层速效钾的块金效应小于25%，其它养分均在25%～75%，具有中等的空间相关性。确定了合理取样数，0～20 cm碱解氮、速效磷、速效钾、交换性钙、交换性镁、有机质合理取样数分别为：5、30、26、29、25、3；20～40 cm碱解氮、速效磷、速效钾、交换性钙、交换性镁、有机质合理取样数分别为：4、44、30、26、25、3，就可以满足变量施肥的要求。