Fractionation of soil humic acids by preparative polyacrylamide gel electrophoresis in the presence of concentrated urea

To separate soil humic acids (HAs) into their constituents and characterize them, polyacrylamide gel electrophoresis (PAGE) was carried out in the presence of 7 M urea using a preparative electrophoresis system. Two types of soil HAs were fractionated into nine fractions by PAGE. The dark-colored constituents were recovered from the electrophoretic fractions by precipitation on acidification, and the brown-colored constituents dissolved in the acidic solution of fast-moving fractions were recovered by adsorption onto DAX-8 resin. High-performance size-exclusion chromatography (HPSEC) confirmed that the constituents of the HAs were separated based on their molecular size by PAGE. The dark-colored constituents exhibited higher degrees of humification than did the corresponding unfractionated HAs, except for the constituents remaining in the electrophoretic gels at the end of electrophoresis. Diffuse reflectance infrared spectroscopy revealed that the chemical properties of the dark-colored constituents changed regularly: the content of carboxyl groups decreased and the proportions of proteinous, aliphatic and polysaccharide moieties increased with increasing molecular size. The humification degrees of the constituents adsorbed onto DAX-8 resin were considerably lower than those of the corresponding unfractionated HAs. The chemical properties of the DAX-8-adsorbed constituents were different from those of the dark-colored constituents. Observation of electrophoretic fractions under blue light (470 nm) and HPSEC with fluorescence detection at an excitation wavelength of 460 nm and an emission wavelength of 520 nm showed that green fluorescent substances were largely concentrated in the smallest molecular size fractions and were partitioned into both the dark-colored precipitates and DAX-8-adsorbed fractions. The proportion of organic carbon recovered by precipitation and adsorption onto DAX-8 resin was 45–63%, indicating that substantial parts of the HA constituents were missing. The unrecovered constituents were considered to be acid-soluble, nearly colorless substances. The dissociation of the acid-soluble constituents from the acid-insoluble dark-colored constituents during the preparative PAGE of soil HAs was ascribed to disruption of hydrogen bonding and hydrophobic interactions caused by concentrated urea.     

Spatial variability of water retention parameters and saturated hydraulic conductivity in a calcareous Inceptisols (Khuzestan province of Iran) under sugarcane cropping

The objective of this study was to quantify inherent spatial variability and spatial cross-correlation of the van Genuchten retention parameters and saturated hydraulic conductivity (K_s) of surface and subsurface layers in a calcareous Inceptisols (Khuzestan province, Iran) under sugarcane cropping. Measurements were performed on 100-cm³ undisturbed soil cores collected at 94 locations along a 30-m-long transect with horizontal sampling distance intervals of 0.3 and 1 m at soil depths of 0–40 and 40–80 cm, respectively. Spatial variability was investigated using conventional statistics and geostatistical techniques. Coefficient of variation (CV) varied from 8.2% (for shape parameter, n at 40–80 cm depth) to 256.7% (for K_s at 0–40 cm depth). The n parameter and saturated water content, θ_s, showed a small-scale spatial heterogeneity with a maximum CV of 11.3% for the first depth and 9.2% for the second depth. Most of the hydraulic parameters at both depths showed a spatial structure and convex experimental semivariograms with dominant spherical models with the influence range of 3.2–41 m. In most cases, the extent of spatial correlation scales of cross-semivariograms for pairs of cross-correlated hydraulic variables was found to be different with reference to those relating to the direct semivariograms of correlated variables.     

不同前处理条件对动态光散射检测酪蛋白胶束粒径的影响

为了准确评价乳的稳定性和加工性能,探讨不同前处理条件对动态光散射检测酪蛋白胶束粒径的影响,研究了稀释液的种类(超纯水、钙咪唑缓冲液、模拟牛乳超滤液和牛乳超滤液)、稀释液温(4和25℃)和稀释液的放置时间(0~48 h)对脱脂乳中酪蛋白胶束粒径的动态光散射测试结果的影响,并将酪蛋白胶束粒径的动态光散射测试结果与冷冻透射电镜图像中测得的真实结果进行比较。研究发现以超纯水和钙咪唑缓冲液作为脱脂乳稀释液时,部分胶束发生解离,影响测试结果;采用牛乳超滤液及模拟牛乳超滤液作为稀释液时,胶束的微环境没有改变,反映了胶束的真实粒径及分布;放置24 h后,牛乳超滤液及模拟牛乳超滤液将产生颗粒;温度对测试有显著的影响(P0.05):4℃的样品用25℃的稀释液进行稀释后,动态光散射的计数率和粒径分别增大了16.6%和11.4%;25℃的样品用4℃的稀释液进行稀释后,计数率和粒径分别降低了16.1%和9.8%。结果表明酪蛋白胶束粒径的测试前处理较适宜的条件为:在与样品的温度相同条件下,以配置好后24 h内的模拟牛乳超滤液或牛乳超滤液(10 k Da超滤膜)作为脱脂乳的稀释液进行稀释。通过与冷冻电镜条件下测得的酪蛋白胶束粒径的真值比较,发现该前处理条件下酪蛋白胶束粒径的动态光散射测试结果的相对误差为-5.7%~1.8%,表明该样品前处理方法可用于动态光散射方法快速检测酪蛋白胶束粒径。研究结果为快速、准确地获取酪蛋白胶束的粒径信息,进而准确分析乳的稳定性及加工性能提供参考。     

东柳沟流域表土粒度分布特征及可风蚀性研究

区域表土粒度分布特征可反映该区环境特征,研究风水复合侵蚀区小流域内部表土粒度分布特征,可为探究风水两相侵蚀作用机理提供一定的理论依据。通过野外表土空间采样和室内测定,对地处风水复合侵蚀区的东柳沟流域的表土粒度分布特征及其可风蚀性进行研究,结果表明:东柳沟流域各种土地利用类型的表土粒径主要为细砂粒(50~250μm),体积分数为56.59%~86.65%,属于风沙土。流域不同土地利用类型表土粒度性质存在显著差异,草地、农地、灌木地的平均粒径(Mz)分别为77.60,81.66,87.85μm,颗粒级配较细,而沙地、河道的Mz分别为126.36,151.80μm,颗粒级配较粗;流域表土粒径大部分表现出正偏和窄峰,颗粒分布较集中。从流域上游到中游,表土粒度表现出明显的粗化现象,具体表现为:除河道外,上游各土地利用类型表土的Mz均显著小于中下游(P0.05)。而在流域中游,除草地、河道之外,其他土地利用类型的Mz与沙地之间没有显著性差异。对流域表土的可风蚀性颗粒分析结果表明,自上游到中游的各土地利用类型的易风蚀颗粒含量显著增加,草地、农地、灌木、河道分别增加了44.4%,54.23%,48.84%,33.76%。结论表明,流域中游是表土粒度分布最复杂、风蚀作用最强烈的区域,同时也是风蚀与水蚀叠加作用最为频繁的区域。     

Stabilization mechanisms of organic matter in four temperate soils: Development and application of a conceptual model

Based on recent findings in the literature, we developed a process‐oriented conceptual model that integrates all three process groups of organic matter (OM) stabilization in soils namely (1) selective preservation of recalcitrant compounds, (2) spatial inaccessibility to decomposer organisms, and (3) interactions of OM with minerals and metal ions. The model concept relates the diverse stabilization mechanisms to active, intermediate, and passive pools. The formation of the passive pool is regarded as hierarchical structured co‐action of various processes that are active under specific pedogenetic conditions. To evaluate the model, we used data of pool sizes and turnover times of soil OM fractions from horizons of two acid forest and two agricultural soils. Selective preservation of recalcitrant compounds is relevant in the active pool and particularly in soil horizons with high C contents. Biogenic aggregation preserves OM in the intermediate pool and is limited to topsoil horizons. Spatial inaccessibility due to the occlusion of OM in clay microstructures and due to the formation of hydrophobic surfaces stabilizes OM in the passive pool. If present, charcoal contributes to the passive pool mainly in topsoil horizons. The importance of organo‐mineral interactions for OM stabilization in the passive pool is well‐known and increases with soil depth. Hydrophobicity is particularly relevant in acid soils and in soils with considerable inputs of charcoal. We conclude that the stabilization potentials of soils are site‐ and horizon‐specific. Furthermore, management affects key stabilization mechanisms. Tillage increases the importance of organo‐mineral interactions for OM stabilization, and in Ap horizons with high microbial activity and C turnover, organo‐mineral interactions can contribute to OM stabilization in the intermediate pool. The application of our model showed that we need a better understanding of processes causing spatial inaccessibility of OM to decomposers in the passive pool.     

Population size and specific potential of P-mineralizing and -solubilizing bacteria under long-term P-deficiency fertilization in a sandy loam soil

The effects of organic manure, mineral fertilizer (NPK), and P-deficiency fertilization (NK) on the individual biomass of young wheat plants, arbuscular mycorrhizal (AM) colonization in wheat root systems, population sizes of soil organic phosphorus mineralizing bacteria (OPMB) and inorganic phosphate solubilizing bacteria (IPSB) as well as soil P-mineralization and -solubilization potential were investigated in a long-term (18-year) fertilizer experiment. The experiment included five treatments: organic manure, an equal mixture of organic manure and mineral fertilizer, fertilizer NPK, fertilizer NK, and the control (without fertilization). Plant biomass, population sizes of soil OPMB and IPSB were greatly increased (P<0.05) by the application of organic manure and slightly increased by the balanced application of mineral fertilizer, while undiminished AM colonization in wheat root system was only observed in the case of the NK treatment. Compared to balanced fertilization, P-deficiency fertilization resulted in a significant increase (P<0.05) of OPMB-specific mineralization potential (soil P-mineralization potential per OPMB cell) and highest IPSB-specific solubilization potential (soil P-solubilization potential per IPSB cell), suggesting that OPMB and IPSB are likely more metabolically active in P-deficiency fertilized soils after long-term fertilizer management, and mycorrhizal plants are more dependent on AM in P-poor soils than in P-fertilized soils. Our results also showed the different effects of mineral fertilizer versus organic manure on soil P-mineralization and -solubilization potentials, as well as specific potentials of OPMB and IPSB in arable soils.     

砒砂岩区不同退耕还林措施土壤颗粒及交换性能分布特征

为研究砒砂岩区退耕还林还草措施下林地和草地的土壤结构及土壤交换性能对其措施的响应,选取柠条林、油松林、小叶杨林和本氏针茅草地为研究对象,并以荞麦坡耕地为对照,通过野外取样与室内试验相结合的方式,采用分形理论探究土壤粒径分布(PSD)、阳离子交换量(CEC)和交换性盐基总量(ECEC)及其组成(Na~+、K~+、Ca~(2+)、Mg~(2+))的分布特征,并分析其相关性关系。结果表明:(1)实施退耕还林措施后,草地和林地的PSD分布范围、非均一性、离散程度均高于坡耕地,且柠条林的土壤粉粒含量、PSD分布范围的增幅效果最显著(P0.05);土壤剖面垂直层次上,草地有利于增加表层土壤的细粒组分和粒径的分布范围,而林地更有利于对深层土壤粒径的改良与细化;(2)研究区交换性盐基组成主要以碱土金属为主(Ca~(2+)、Mg~(2+)),不同措施的土壤ECEC和CEC值由大到小依次为柠条林油松林小叶杨林草地坡耕地。草地表层土壤交换性能优于底层土壤,而林地与之相反;(3)黏、粉粒和细砂是决定研究区土壤交换性能的细粒土壤和粗粒土壤,粉粒是CEC、ECEC的主要贡献因子,多重分形维数可较好地描述土壤交换性能与土壤颗粒间的关系。不同措施以柠条林对土壤颗粒组成和土壤交换性能的改良效果最优。     

中国5种典型土壤的侵蚀泥沙粒径分布特征

通过天然降雨试验,研究我国5种典型土壤的侵蚀泥沙团粒和单粒粒径分布以及泥沙各级团粒的机械组成特征。2013—2014年汛期(6—9月),监测红壤、紫色土、黄绵土、褐土和黑土径流小区的降雨产流事件,收集侵蚀泥沙样品,利用湿筛法和吸管法测定其团粒和单粒粒径分布;同时,筛选出泥沙各级团粒样品,进一步测定其单粒粒径组成。结果表明:(1)侵蚀泥沙粒径分布是土壤质地和降雨强度综合作用的结果,团粒粒径分布更适合作为表征泥沙输移的指标;(2)5种土壤的侵蚀泥沙皆表现出粉团(0.002~0.05mm)和黏团(0.002mm)的明显富集;(3)在泥沙单粒粒径分布方面,除红壤的粉粒(0.002~0.05mm)和黏粒(0.002mm)有轻微富集外,其余4种土壤的各级单粒均无明显富集;(4)红壤侵蚀泥沙粉团(0.002~0.05mm)中的黏粒含量最多,其余4种土壤侵蚀泥沙机械组成和各级团粒的机械组成均与原土接近。     

风水复合侵蚀下锡林河流域不同管理方式 草地表土粒度特征

结合锡林河流域风水侵蚀情况,对锡林河流域围封天然草地、放牧草地、封育种植草地表层(0~5 cm)土壤粒度特征及其与风蚀、水蚀关系进行研究,结果表明:封育种植草地较放牧和天然草地在降低土壤风蚀和水蚀方面均起到了较明显的作用;锡林河流域草地表层土壤粒度组成以砂粒为主,其所占比例为75%~82%,放牧草地较天然草地及封育种植草地土壤粒度粗化明显,封育种植草地中蒙古冰草+草木樨草地土壤颗粒分选性较差,其余草地土壤颗粒分选性均为中等;研究区内粒径在160μm左右土壤颗粒为易受侵蚀土壤颗粒;研究区土壤粒度组成特征与风蚀、水蚀均有明显相关性,尤其与近地面20 cm风速和径流产流总量联系最为密切。     

高度对色斑法测量雨滴粒径影响的试验研究

以前的色斑法雨滴率定只考虑色斑直径与雨滴真实粒径的关系，忽略了高度对率定的影响。在大量试验的基础上，通过回归分析，得到了以色斑直径和高度为变量的率定公式。研究表明，水滴下落高度小于5m时需考虑高度的影响；高度大于5m时，高度的影响可以忽略。通过相关性分析，公式的拟合性较好，对以后的色宽法测量雨滴粒径有指导意义。