甜叶菊种传病害防治方法的研究

采用种子处理方法可显著提高种子发芽率、降低发病率；其中以药剂浸种效果最好；药剂处理以多菌灵＋福美双和多菌灵两个配方最佳。     

Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle: I. PCR Screening and Allele Frequency Estimation

Jergensen, C. B., J. S. Agerholm, J. Pedersen and P. D. Thomsen: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian Cattle I. PCR screening and allele frequency estimation. Acta vet. Scand. 1993,34,231-236.–A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.     

USE OF ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION BIOPSY AND AUTOMATED CORE BIOPSY FOR THE DIAGNOSIS OF GASTROINTESTINAL DISEASES IN SMALL ANIMALS

The purpose of this study was to evaluate the usefulness of ultrasound-guided fine-needle aspiration biopsy and core biopsy in the diagnosis of infiltrative gastrointestinal diseases. Six dogs and seven cats with clinical signs of gastrointestinal disease underwent ultrasonography and intestinal lesions were identified. One or more ultrasound-guided fine-needle aspiration biopsy and/or core biopsy procedures were performed in each patient. Each patient also underwent one of the following additional procedures for comparison of results: 1) surgery (n=4), 2) endoscopy (n=2), 3) post mortem exam (n=3), and, 4) for lymphoma diagnosed with ultrasound-guided procedures, response to chemotherapy (n=4). Correct diagnoses were obtained in nine of the 13 patients, incorrect diagnoses in two of the 13 patients, and inconclusive diagnoses in two of the thirteen patients. Of the 16 total ultrasound-guided procedures performed, ten were confirmed as correct, three as incorrect, and three were non-diagnostic. Intestinal lesions with bowel wall thickness greater than 2.0 cm had a higher percentage of correct diagnoses than lesions of lesser wall thickness. Gastric lesions had a higher percentage of correct diagnoses than small and large intestinal lesions. Malignant lesions had a higher percentage of correct diagnoses than benign lesions. There were no complications. Ultrasound-guided gastrointestinal fine-needle aspiration biopsy and core biopsy appears to be a safe, accurate, and rapid procedure for use in the diagnosis of infiltrative gastrointestinal disease.     

重迎茬亚麻生长发育障碍机制的研究

重迎茬亚麻减产是由多种因素综合作用的结果,亚麻重迎茬可使病害加重是最主要因素,重茬年限越久发病越严重;重迎茬造成了土壤营养元素的单一消耗,随着重迎茬年限的增加,土壤中速效氮、速效磷、速效钾等含量降低。     

有机大米加工技术规程

本文根据内蒙古赤峰市翁牛特旗有机大米加工实践及国内外先进经验,总结提出一整套有机大米加工技术规程。包括原料要求,加工厂址、建设要求,加工设备、加工人员、加工方法、质量要求及管理等系列化要求标准,为有机大米加工业建设提供了参考。     

超级稻“三定”栽培法研究Ⅲ．不同栽培方式对超级杂交稻产量形成的影响

超级杂交稻的优化(稀植、结构施肥)栽培试验于2002-2004年在长沙进行,以比较不同栽培方法对超级杂交稻产量及物质生产的影响。以两优培九为材料,并以汕优63作为对照。结果表明,两种栽培法,两个供试品种的产量表现不同,其中两优培九采用优化栽培单产为8.20～10.37t/hm2,比传统栽培增产显著。主要表现为有效穗多,而结实率、千粒重、穗实粒等产量因子差异不明显。汕优63采用优化栽培单产比传统栽培减产0.37%～8.8%。两种栽培方法间的茎蘖发生动态和单株分蘖数存在极显著差异,两组合单株分蘖数优化栽培比传统栽培分别多110.36%和110.64%,但由于移栽密度不同,两种栽培方式间的单位面积分蘖数没有明显差异。两优培九采用优化栽培的在各个生育时期,植株体内的含氮量比传统栽培的高。     

火鹤花衰退病诊断与检疫

本文描述了火鹤衰退病病害症状和2种穿孔线虫(香蕉穿孔线虫Rodopholus sim-ilis和柑橘穿孔线虫R．citrophilus)的鉴定特征，提出了检疫检验和疫情处理方法。     

黑鲷幼鱼腹水病病原菌

2001年9月自浙江省宁波市西沪港海区网箱养鱼场养殖黑鲷濒死鱼体内分离到1株细菌，经人工感染试验证明是黑鲷幼鱼腹水病病原菌，其主要特征为：革兰多染色阴性，短杆状，极生单鞭毛，氧化酶阳性，发酵葡萄糖产酸不产气，经SWF（A）系统鉴定为拟态弧菌。该菌株对磺胺甲基异恶唑、呋喃妥因、复方新诺明、氯霉素、头孢孟多、丙氟哌酸等药物敏感。     

奶牛乳房炎多联苗(B)的临床免疫试验

用奶牛乳房炎多联苗 (B)免疫家兔 ,14 d血清中金黄色葡萄球菌抗体效价可达 1∶ 8,2 8d抗体水平明显升高 ,可达 1∶ 32 ,注苗后 35 d攻毒 ,攻毒后 2 d保护率为 93.3% (14 /15 ) ,自然保护率为 2 0 % (1/5 ) ,10 d保护率为 6 6 .6 % (10 /15 ) ,自然保护率为 2 0 % (1/5 ) ;用多联苗 (B)臀部肌肉注射免疫泌乳牛 ,试验期间 4个月内 ,3ml剂量组临床型乳房炎月平均发病率为 11.0 9% ,其对照组为 2 0 .19% ,发病率降低 4 5 .2 9% ,差异极显著 (P<0 .0 1) ;5 m l剂量组月平均发病率为 13.0 1% ,其对照组为 31.36 % ,发病率降低 5 8.5 1% ,差异极显著 (P<0 .0 1) ;3ml剂量组与 5 m l剂量组之间发病率无明显差异 (P>0 .0 5 ) ;注苗后泌乳牛血清抗体水平可持续 4个月 ,且 30 d时效价水平最高。     

鸡马立克氏病琼扩抗原的研制及应用

将马立克氏病死鸡的皮肤或羽囊捣碎，制成MD琼扩抗原。试验结果表明，自制MD皮肤和羽囊琼扩抗原与标准抗的检测MD抗体的敏感性和特异性相同，但皮肤琼扩抗原制造方法相对比较简单，产量也较高。