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11.
AIM: To develop and validate a simple and sensitive method using liquid chromatography-mass spectrometry (LC-MS) for quantification of articaine, and its major metabolite articainic acid, in plasma of red deer (Cervus elaphus), and to investigate the pharmacokinetics of articaine hydrochloride and articainic acid in red deer following S/C administration of articaine hydrochloride as a complete ring block around the antler pedicle.METHODS: The LC-MS method was validated by determining linearity, sensitivity, recovery, carry-over and repeatability. Articaine hydrochloride (40?mg/mL) was administered S/C to six healthy male red deer, at a dose of 1?mL/cm of pedicle circumference, as a complete ring block around the base of each antler. Blood samples were collected at various times over the following 12 hours. Concentrations in plasma of articaine and articainic acid were quantified using the validated LC-MS method. Pharmacokinetic parameters of articaine and articainic acid were estimated using non-compartmental analysis.RESULTS: Calibration curves were linear for both articaine and articainic acid. The limits of quantifications for articaine and articainic acid were 5 and 10?ng/mL, respectively. Extraction recoveries were >72% for articaine and >68% for articainic acid. After S/C administration as a ring block around the base of each antler, mean maximum concentrations in plasma (Cmax) of articaine were 1,013.9 (SD 510.1) ng/mL, detected at 0.17 (SD 0.00) hours, and the Cmax for articainic acid was 762.6 (SD 95.4) ng/mL at 0.50 (SD 0.00) hours. The elimination half-lives of articaine hydrochloride and articainic acid were 1.12 (SD 0.17) and 0.90 (SD 0.07) hours, respectively.CONCLUSIONS AND CLINICAL RELEVANCE: The LC-MS method used for the quantification of articaine and its metabolite articainic acid in the plasma of red deer was simple, accurate and sensitive. Articaine hydrochloride was rapidly absorbed, hydrolysed to its inactive metabolite articainic acid, and eliminated following S/C administration as a ring block in red deer. These favourable pharmacokinetic properties suggest that articaine hydrochloride should be tested for efficacy as a local anaesthetic in red deer for removal of velvet antlers. Further studies to evaluate the safety and residues of articaine hydrochloride and articainic acid are required before articaine can be recommended for use as a local anaesthetic for this purpose. 相似文献
12.
Gas and short‐chain fatty acid production from feeds commonly fed to red deer (Cervus elaphus L.) and incubated with rumen inoculum from red deer and sheep
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Andrej Lavrenčič Darko Veternik 《Journal of animal physiology and animal nutrition》2018,102(5):1146-1153
Efficient red deer supplementary feeding depends on estimations of the nutritive value of offered feeds, frequently estimated with the use of equations derived from domestic ruminants. The aim of this study was to compare the 24‐hour in vitro true dry matter degradability (ivTD24), in vitro gas production (GP) kinetic parameters, GP in 24 hr of incubation (GAS24) and short‐chain fatty acid (SCFA) and microbial biomass (MBS) produced after 24‐hour incubation of feeds in inoculum prepared from sheep and red deer rumen fluid. Eleven feeds, frequently consumed by red deer in Slovenia, which occur either naturally (two fresh grasses, chestnut fruits and common and sessile oak acorns) or are fed as winter supplemental feeds (two grass hays, two grass silages, apple pomace, fresh sugar beetroot), were investigated. The in vitro GP kinetic parameters, GAS24 and ivTD24, did not differ between animal species. Amounts of SCFAs were greater (p < 0.05) when feeds were incubated in sheep inoculum, while molar proportions of acetic and propionic acids did not differ. Molar proportions of butyric acid produced during incubation of high fibre feeds did not differ between animal species, but were higher (p < 0.05) when feeds high in starch or sugar were incubated in red deer inoculum. Greater production of SCFA by sheep rumen microbes suggests better coverage of host animal with energy precursors, while greater production of MBS by red deer rumen microbes suggests better coverage of host animal with protein. Results also suggest that rumens of sheep and red deer are inhabited by different microbial communities, which did not affect the extent of in vitro GP and degradation of feeds used in the present experiment. However, the possibility exists that the divergent nutrient use could be a consequence of different priming by different feeds of the donor animal diets. 相似文献
13.
为研究红壤微生物丰度和群落组成对不同调酸剂的响应,分析影响碳/氮关键代谢过程微生物的变化,通过盆栽实验,设置不施肥(CK)、钙镁复合剂(L)、钙镁复合剂配施猪粪(ML)和钙镁复合剂配施秸秆(SL)4个处理,采用宏基因组测序技术,分析土壤细菌、真菌和古菌以及碳/氮代谢关键过程微生物。结果表明:L、ML和SL处理显著提高土壤pH值和交换性钙/镁,显著降低土壤交换性酸。调酸剂增加了细菌优势菌中的变形菌门相对丰度,降低了绿弯菌门和酸杆菌门相对丰度;降低了真菌优势菌中的毛霉菌门相对丰度;增加了古菌优势菌中的广古菌门和深古菌门的相对丰度,降低了奇古菌门的相对丰度。冗余分析结果显示,速效钾是影响土壤细菌和真菌群落结构的主要环境因子,土壤pH和有机碳是影响土壤真菌和古菌群落结构组成的关键因子。碳代谢过程的贡献度方面,变形菌门的贡献度在SL处理中最高,放线菌门和芽单胞菌门的贡献度在ML处理中最高。氮代谢过程中,各处理绿弯菌门对硝化作用的贡献率均超过80%。调酸降低了绿弯菌门和酸杆菌门在反硝化与硝酸盐异化还原过程中的贡献度,L与SL处理的变形菌门贡献度低于ML处理,而ML处理的放线菌门贡献度高于L与SL处... 相似文献
14.
陆地棉亚红株突变的质量遗传规律研究 总被引:10,自引:0,他引:10
分析了陆地棉亚红株突变体的淡红叶性状与当日红花性状的质量遗传规律,结果显示,淡红叶性状是受一对不完全显性基因控制的质量性状。连锁分析表明:淡红叶性状与当日红花性状完全连锁,是受同一对显性基因控制的质量性状;淡红叶与棕絮性状符合两对性状完全独立分离规律,不存在连锁遗传关系。等位性测定显示,淡红叶性状与经典红叶性状杂交后代,符合两对显性基因控制的独立分离理论比例,表明控制亚红株突变的基因与控制经典红叶的R1基因不在同一基因位点。根据以上试验结果,推定陆地棉亚红株突变是一个新的质量突变性状,暂将它的基因符号定为RS。 相似文献
15.
将红色文化和旅游相融合,形成红色旅游是新时代旅游业升级转型之路。红色旅游对政治、经济、社会和文化有着较高的价值和效益,但存在品牌效应弱、基础设施不完备、同质化现象严重、服务和管理水平规范化不足等问题。在分析红色旅游内在价值和发展现状及困境的基础上,提出构建新型“红色旅游+乡村振兴”发展模式、沉浸式旅游体验、挖掘红色精神时代价值和推进人才队伍建设等建议,以期为红色旅游的健康发展提供政策支持。 相似文献
16.
Eastern grey squirrels (Sciurus carolinensis) and North American red squirrels (Tamiasciurus hudsonicus) were studied among wooded patches within an agricultural mosaic. Fifteen sites south of Ottawa, Canada, with differing landscape and local features were censused using tracking boards placed in a woods or wooded fencerow. Regression analyses of landscape compositional and physiognomic variables within a 1-km radius isolated the best predictors of grey and red squirrel abundance and activity. Grey squirrels were found in both small woods and fencerows in farm landscapes but were not found in large woods. A polynomial regression of wooded patch size explained 79% of the variance in grey squirrel abundance. Grey squirrel activity was correlated with the percent cover of soybeans in the landscape. Red squirrels were found in fencerows, small and large woods; activity was correlated with the percent cover of both woods and corn crop in the surrounding landscape. These results indicate that distributions of both species are influenced by multiple landscape elements, but that grey squirrels may rely on fragmented agricultural landscapes whereas red squirrels make more use of both native woodland and altered landscapes. 相似文献
17.
赤潮研究中圆海链藻实时荧光定量PCR检测方法的建立 总被引:3,自引:0,他引:3
为建立快速、准确的鉴定和定量检测赤潮生物的方法,以圆海链藻为例,以其18S rDNA序列为寻找种特异性引物的靶区域,通过分析18S rDNA序列,设计出适合用于RFQ-PCR的引物与探针,并通过引物PCR验证确定其特异性,进而以圆海链藻荧光定量PCR的引物和探针(Primer Thalassiosira rotula和Taqman Thalassiosira rotula),建立了定量检测圆海链藻的实时荧光定量PCR检测方法(RFQ-PCR)。与传统的显微镜计数方法比较,两者所获结果无显著性差异,证明了本方法的可行性,从而为我国沿海水域赤潮问题的研究提供了良好的技术检测途径。 相似文献
18.
兴国县红壤颗粒分形及其与环境因子的关系 总被引:4,自引:2,他引:2
以土壤颗粒组成数据为基础,运用分形模型,分析了红壤丘陵山区林地土壤颗粒的分形维数。结果表明:84个耕层土壤颗粒的分形维数D为2.568~2.828,其中紫色土2.722,红壤2.700,棕红壤2.693,黄红壤2.670,黄壤2.713。D随土壤质地的变细而增大。从空间分布上看,研究区域的西部和东南部D值较大,而西南部和北部D值较小,D在2.7~2.8的面积最大,为1171km^2,占总面积的47.0%,D在2.8以上的面积最小,为48km。,占总面积的1.9%。土壤分维数和坡向、海拔之间呈显著正相关关系,而与坡度、平面曲率、剖面曲率之间无明显的相关关系。 相似文献
19.
红壤干旱过程中剖面水分特征与土层干旱指标 总被引:1,自引:0,他引:1
农田水分管理以及产量评估都需要对土壤作物受旱状况定量化和指标化。干旱强度和干旱程度结合才能完整地描述土壤作物干旱状况,土壤干旱强度I是土壤剖面失水速率的函数,干旱影响逐渐累积并增强就构成干旱程度D,据此提出了包含I和D二个指数的土层干旱指标表达模式。通过红壤小区种植玉米并在抽穗期开始设置连续干旱12~36 d等6个不同的处理,研究了红壤干旱过程中剖面水分特征和干旱指标。结果表明:供试红壤干旱过程中剖面40 cm以下含水率下降幅度很小,玉米主要利用了0~40 cm土层的水分,监测30~40 cm土层含水率的变化情况可以指示玉米受到干旱胁迫的程度。连续干旱25 d后40 cm以下土层含水率明显降低,玉米产量也显著下降,此时0~60 cm土层和30~40 cm土层的干旱程度D均为0.55,可用此指标作为灌溉的依据。 相似文献
20.
油菜红边特征及其叶面积指数的高光谱估算模型 总被引:22,自引:6,他引:16
在2003~2004年油菜生长季,选用6个油菜品种,设置3个氮素水平的小区试验。在不同发育期同步测定油菜冠层的光谱反射率及对应叶片的叶面积指数。利用油菜冠层的光谱反射率数据提取红边参数,分析其变化规律,油菜叶面积指数与红边参数的相关性,估算结果表明:油菜冠层红边一阶导数光谱具有“双峰” 现象,红边位置λred位于690~720 nm之间,在油菜生长旺盛期间出现“红边平台”,前期有“红移”,后期有“蓝移”现象;叶面积指数与冠层光谱红边参数λred、Dλred、Sred之间在开花前存在显著相关,但开花后相关性不显著;利用开花前的红边参数可以估算油菜的叶面积指数,开花后的红边参数不能用于估算油菜的叶面积指数;最后建立了不同时期和开花前油菜叶面积指数的估算模型。 相似文献