车前草穗枯病菌分子鉴定

采用真核生物通用引物ITS1/ITS4,对来自江西省吉安县2个车前草穗枯病菌菌株进行rDNA-ITS区段的PCR扩增,对扩增产物进行测序,结果获得2个长度均为579 nt且序列完全一致的rDNA-ITS序列,该序列与GenBank中当归间座壳菌(Diaporthe angelicae)及其无性态拟茎点霉菌(Phomopsis subordinaria)对应序列的同源性高达99.0%,根据以rDNA-ITS序列同源性大小划分种类的标准,将车前草穗枯病菌鉴定为当归间座壳菌。     

Efficacy of different temperate pasture species to reduce nitrogen leaching from cattle urine applied in different seasons: A soil lysimeter study

A soil lysimeter field study assessed the efficacy of different pasture species to reduce nitrogen (N) leaching loss from cow urine deposited in different seasons. A single application of cow urine (¹⁵N‐labelled; equivalent to 622 kg N ha^?1) was applied in three different seasons (summer, autumn or winter) to three pasture species monocultures (perennial ryegrass, plantain or lucerne) on a free‐draining volcanic soil and monitored over 362 days. Leachate analyses revealed consistently large leaching losses of inorganic‐N from lucerne (>200 kg N ha^?1) across different urine application times due to the relatively low plant growth rates during winter (<15 kg DM ha^?1 day^?1) that led to low total recovery of urine‐N by lucerne plants (<20% of the applied urine‐¹⁵N). Conversely, plant uptake of the urine‐N was higher by plantain (ranging from 30% to 45% of that applied) driven by moderately higher winter plant growth rates (30 to 60 kg DM ha^?1 day^?1). Plantain exhibited large seasonal variation in its efficacy to reduce urine‐N leaching relative to ryegrass (ranging from 15% to 50% reduction for summer or winter urine applications, respectively) with an overall reduction of 39% in the total amount of inorganic‐N leached across the three seasons (53 vs. 87 kg N ha^?1 leached relative to ryegrass). This study has demonstrated the potential benefit of using plantain to reduce N leaching losses from urine deposited in the summer to winter grazing period. However, further research is required to quantify the effects of plantain on annual N leaching losses from grazed pastoral systems.     

Cytoplasmic genetic diversity in bananas and plantains

Summary Concerns over yield declines in bananas and plantains due to the spread of Black Sigatoka disease in Musa have drawn attention to the collection of Musa germplasm and its use in conventional and biotechnological improvement programs. This report demonstrates the use of chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) for differentiating cytoplasms of various Musa clones. DNA was extracted from lyophilized leaf blade tissue and digested with either Eco R1, Hind III, Bam H1 or Pst I. Southern blots onto nylon membranes were probed with radioactively labeled heterologous orchid and lettuce cpDNA fragments. Among the 14 Musa clones examined, a single balbisiana and four acuminata-type cytoplasms were differentiated. The ability to distinguish between cytoplasms and to place plants within a cytoplasmic grouping demonstrates the usefulness of RFLP technology in evaluating diversity and determining the ancestry of Musa clones.     

Molecular breeding in the genus Musa: a strong case for STMS marker technology

Musa species are among the tallest monocotyledons and include major food-producing species. The principal cultivars, derived from two major species Musa acuminata (‘A’ genome) and Musa balbisiana (‘B’ genome), are polyploid hybrids (mainly AAA, AAB and ABB triploids), medium to highly sterile, parthenocarpic and clonally propagated. Bananas and plantains are crops to which molecular breeding is expected to have a positive impact. In order to better understand banana genetics, more knowledge has to be accumulated about the complex genome structure of hybrids and cultivars. Therefore, the aim of our work is to develop molecular markers that are codominant, reliable, universal, highly polymorphic and that are applicable to collaborative Musa germplasm genotyping and mapping. Two size-selected genomic libraries have been screened for the presence of simple sequence repeats (SSR). Our data demonstrate that SSR are readily applicable to the study of Musa genetics. Our comprehensive analyses of a significant number of banana sequence tagged microsatellite sites (STMS) will add to our knowledge on the structure and phylogeny of genomes of the Musa species, and suggest that microsatellites be used as anchor markers for a banana genetic core map. Additional markers, such as e.g. CAPS have also been tested in order to increase the detection of polymorphisms exceeding that revealed by STMS technology. The utility of PCR-derived markers for collaborative genetic analyses of the banana genome, and the transferability of 'streamlined’ laboratory techniques and data analysis to Developing Countries are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.     

Major polyphenols in aerial organs of greater plantain (Plantago major L.), and effects of drying temperature on polyphenol contents in the leaves

Plantago major L. (common plantain) has been used in folk medicine all over the world, mainly for the healing of wounds. This species already has a high market demand in Asia, and attempts to domesticate P. major as a medicinal crop in horticulture are emerging also in Europe. Both polysaccharides and polyphenols have been proposed to have bioactive effects. In this study, major phenols were analysed in leaves, flower stalks and seeds of P. major plants from five homogenous offspring families, as verified by DNA-marker analysis. Furthermore, leaves were harvested and dried at different temperatures. The contents of plantamajoside, verbascoside and a hitherto unidentified compound, P1, differed greatly between plant organs. Degradation of leaf polyphenols increased with an increase in drying temperature, and consequently, freeze-drying resulted in the highest content of phenols. Thus it is important to select plant material of a suitable organ or combination of organs, and to use the lowest possible drying temperature to obtain a high content of a specific phenolic compound in P. major.     

Hand pulling of Striga hermonthica in pearl millet∗

Abstract

Before 1960, Panama disease (fusarium wilt) was the most important disease in the banana export trades. Although the importance of Panama disease decreased after the Cavendish cultivars replaced Gros Michel, recent outbreaks have renewed interest in this problem. Race 4 affects cultivars of the Cavendish subgroup in the subtropics, as well as race 1 and race 2 suscepts. It has caused concern in the tropically based export trades which depend almost entirely on the Cavendish clones. Attention is also drawn to affected non‐exported bananas which are important cash crops or staple foods. Although they are not as well documented as those on the Cavendish subgroup, epidemics of the disease on non‐exported bananas are far more important.     

Influence of Potassium Fertilizer on Yield of Plantain Intercropped with Cassava on an Oxic Paleustalf in Southwestern Nigeria

Abstract

There is very little literature on the influence of plantain–cassava intercropped on this soil type. Therefore, an experiment was conducted in 1993 and 1995 cropping seasons on the influence of potassium (K) fertilizer on the yield of the two crops on a soil classified as Oxic paleustalf (or Ferric Luvisol) in the rain forest agroecological zone of Nigeria. Four rates of K fertilizer (120, 240, 360, and 480 kg K ha^?1) and control were evaluated on the yield and yield components of these two crops in sole and intercropping. The experimental design was a randomized complete block (RCBD) replicated four times. Results showed that the soil is acidic and the exchangeable cations contents were suboptimal when compared with the optimum required for a sustained cultivation of plantain. Furthermore, the plantain–cassava intercropped yields responded significantly (p<0.05) to fertilizer applied in terms of the yield and yield components of both crops in sole and intercropping. Highest bunch weight of plantain and tuber yield of cassava was recorded at 360 and 240 kg K ha^?1 (taking the mean of both years). Potassium fertilizer after this point resulted in lower yields of both crops when intercropped.     

Comparison of the bioactive components of the ecotypes and cultivars of plantain (Plantago lanceolata L.) herbs

The concentrations of the bioactive components, acteoside, aucubin and catalpol, and the production yield, crude protein (CP) and crude ash contents in 25 ecotypes (Et) of plantain (PL, Plantago lanceolata L.), found in northern Japan, and commercial cultivars, Grasslands Lancelot (GL) and Ceres Tonic (CT), were investigated. The quantitative determination of bioactive components was performed by high performance liquid chromatography. The acteoside content of some of the 25 Et was significantly higher (P < 0.05) than CT and numerically higher than GL and other Et. Most of the Et had numerically higher concentrations of catalpol and aucubin than the GL and CT. The CP and crude ash content of the Et were comparable with those of the GL and CT. The dry matter (DM) yields of some of the Et was numerically greater than that of the cultivars. The present data suggest that most of the Et are comparable to the cultivars in respect to the concentration of bioactive components and DM yield as well as CP and crude ash contents and could be used as pasture like GL and CT.     

“double bunch” in a plantain germplasm in Nigeria: a new Genotype

In the course of germplasm exploratory work, a strain of a False Horn plantain cultivar was found which produced two bunches at fruiting. Cropping of the suckers of this material through three cycles showed that all the plants studied, produced two bunches of fruits. The results suggest that this plantain tentatively designated as double bunch, is of genetic origin arising probably from a mutation of the existing plantain cultivar. The implications for the identification of genetic diversity as well as for the evaluation and conservation of genetic resources are discussed.     

大蕉果实呼吸和催熟实验的教学与实践

选择呼吸跃变型、酶褐变迟缓的大蕉果实作为原料,针对大蕉果实呼吸强度和人工催熟的特点,通过归纳和设计性的实验教学,使学生的综合性素质得到提高。