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111.
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中国栽培平菇的酯酶同工酶分析 总被引:14,自引:0,他引:14
采用电泳技术对我国大规模栽培的64个平菇商业菌株进行酯酶同工酶分析。结果表明,我国栽培平菇菌种多样性丰富,64个菌株有44个酶谱类型,不同菌株有各自特有的酯酶同工酶酶谱。聚类分析表明,供试菌株在60%相似水平上分为6大类:第一类包括以白黄侧耳为代表的21个菌株;第二类包括以德国引进菌株为代表的6个未定种名菌株;第三类包括7个白黄侧耳与佛罗里达侧耳杂交的杂交种及4个未定种名菌株;第四类包括以佛罗里达侧耳为代表的5个菌株;第五类包括以糙皮侧耳为代表的14个菌株;第六类包括以肺形侧耳为代表的7个菌株。 相似文献
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B.C. Lee Y.K. He K. Murao M. Isogai G. Dahal I. Uyeda 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(6):493-499
Rice dwarf virus isolates were collected from several locations in Japan, the Philippines, China, Nepal and Korea. Genomic dsRNA segment profiles in polyacrylamide gel electrophoresis differed among the isolates. There were less differences in the profiles between isolates from Japan and Korea than in those between these two Countries and others. Nucleic acid hybridization was used to examine the extent of genomic variation. Full-length cDNAs to all genomic segments encoding non-structural proteins (S4, S6, S9, S10, S11 and S12) were synthesized from two Japanese isolates, and were used for dot-blot hybridization. Hybridizations using probes generated from the full-length cDNA clones failed to differentiate isolates from different geographical areas. However, cDNA probes covering a variable region of S12 were able to distinguish Japanese and Korean isolates from those of other countries. Phylogenetic tree analysis based on the amino acid sequence of P12 encoded by S12 grouped Japanese and Korean isolates together. The Chinese isolates from two different locations (Yunnan and Fujian) were closely related to each other, and were the most distantly related to Japanese and Korean isolates. 相似文献
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引进丹麦先进仪器FAT—O—MEAT’ER(肉脂仪)对中国鲁梅克斯食品总公司名山县屠宰场中5821头猪进行了现场测定,测量性状为最后一根肋骨处离背中线4~5cm处的膘厚(P2)和倒数第三、四根肋骨之间,离背中线4—5cm处的膘厚(RF)以及眼肌厚度(RM),并估测出瘦肉率。对所测胴体各性状的均数和相关性分析,表明本地商品肉猪胴体质量低于国外水平。 相似文献
117.
猪大肠杆菌水肿毒素SLT-IIeA基因的克隆和序列分析 总被引:2,自引:0,他引:2
研究以本地猪水肿病大肠杆分离物ED1株为材料 ,利用 PCR克隆了含猪水肿病大肠杆菌 (VTEC) slt-IIe A基因 987bp的片段 ,并测定了含该克隆片段的 Bam HI、Hind 酶切片段的核苷酸序列。结果表明 ,slt-IIe A基因的编码区全长 960 bp,编码 3 1 9个氨基酸的蛋白质 ,序列与国外报导的 S1 1 79株进行比较发现其核苷酸同源性为 98.9%。经推导的氨基酸序列的同源性为 99.7%。这为进一步研究 slt-IIe A的生物学特性、水肿病的分子诊断及其防制打下基础 相似文献
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Maurice Keith R. Welch Joan M. Brown Christopher P. Latham Roger Earl 《Landscape Ecology》2004,19(6):603-620
The Pocono mesic till barrens (PMTB) are a unique assemblage of fire-maintained shrub communities that support numerous rare species. Historically these barrens covered a large area in the vicinity of Long Pond, Pennsylvania, USA. However, due largely to regional fire suppression instituted in the early 1960s, over 70% of the area covered by barrens succeeded to fire-intolerant forest that does not support the rare species. We investigated the influence of forest proximity on barrens succession across three geomorphic types during periods of high fire frequency and fire suppression, testing the hypothesis that forest processes such as seed rain, shading, and detrital enrichment of soils enhances barrens succession through a contagion effect. Evidence of a forest contagion effect should be shown by increased rates of barrens succession with increasing proximity to the nearest forest edge. In order to detect a forest contagion effect, barrens persistence and barrens succession were modeled in proximity zones of 0-50 m, 50-100 m, 100-200 m, and greater than 200 m from the nearest forest edge. We used existing GIS data layers for fire, geomorphology, and vegetation distribution in 1938, 1963, and 1992. The layers were modified and overlain using ArcView software to determine persistence and succession rates for each unique combination of layers in each proximity zone from 1938 to 1963 (pre-fire suppression) and 1963 to 1992 (post-fire suppression). ANCOVA results indicate that proximity to the nearest forest edge significantly affected barrens persistence rates in both time periods, but succession rates were significantly affected in 1938 to 1963 only. Twenty-eight percent of the 1938 barrens succeeded to forest by 1963; 56% of the 1963 barrens became forest by 1992. Results support previous findings that barrens persistence is enhanced by increased fire frequency, and that barrens persist longer where they overlie flat glacial till than on other geomorphology types. 相似文献