Estimating effective soil depth at regional scales: Legacy maps versus environmental covariates

Soil depth reflects the quantity and ecosystem service functions of soil resources. However, there is no universal standard to measure soil depth at present, and digital soil mapping approaches for predicting soil depth at the regional scale remain immature. Using observation of soil profile morphology, we compared the soil depth nomenclatures from the World Reference Base for Soil Resources, Chinese Soil Taxonomy, and Soil Taxonomy. For this study, shallow soils were defined as those with an effective soil depth < 100 cm. Based on legacy data and field soil survey, the spatial distribution of shallow soils in Xinjiang, China, and the main controlling environmental factors were explored. Results showed that shallow soils in Xinjiang are mainly distributed in high altitude regions such as the Tian Mountains. At the regional scale, significant correlations were observed between soil depth and climate factors, as well as between soil depth and vegetation fractional coverage. Contrary to previous conclusions at small spatial scales, terrain attributes could not explain soil depth variation at the regional scale. This study addressed knowledge gaps on soil depth prediction at regional scales while elucidating climate‐vegetation‐soil coevolution.     

早熟陆地棉主要株型性状与产量的相关性

【目的】分析早熟陆地棉主要株型性状与产量的相关性,为新疆早熟陆地棉品种选育提供科学依据。【方法】采用SPSS 19.0软件,对早熟陆地棉品种的籽棉产量与株型性状进行相关性分析、通径分析和逐步回归分析。通过对产量与株型性状的逐步回归分析,得出产量与株型性状之间的最优回归模型。【结果】对早熟陆地棉籽棉产量有直接作用且有极显著影响的因子(P＜0.01),依次是:株高(X₁)、铃数(X₄)、始节位(X₂),对产量(Y)的直接相关系数分别为:0.322 1、0.298 1、-0.216 2;果枝数(X₃)对Y值直接作用较小,系数为0.082 04,通过其他因子对Y值的间接作用较大,简单相关系数为0.403,相关性极显著(P＜0.01)。早熟陆地棉株型性状(X_i)对产量(Y)的最优回归方程:Y=173.898+2.279 X₁-17.632 X₂+21.795 X₄。估测值与实测值之间的相关程度为0.527,决定系数R²为0.278。【结论】株高、铃数、果枝始节位对产量性状有直接作用,且作用显著;而果枝台数对产量有间接作用,且作用显著。     

山西普通菜豆初级核心种质农艺性状遗传多样性研究

本研究通过对152份山西省普通菜豆初级核心种质进行农艺性状鉴定及农艺性状多样性分析,旨在明确山西省普通菜豆种质资源的主要类型,各类型及性状的主要农艺性状特征及多样性特点。通过在田间进行农艺性状鉴定,同时利用表型数据,采用聚类分析、多样性分析和主成分分析法,确定了山西省普通菜豆种质资源的主要类型,各类型和各性状的农艺性状特征和多样性特点以及24个表型性状所属的主成分。结果表明山西普通菜豆初级核心种质可划分为5种类型。从各分组多样性来看,半蔓+蔓生-紫茎紫花型和蔓生-籽粒斑纹型多样性指数I最高,而直立型资源多样性指数I最低,从各性状多样性来看,质量性状多样性总体低于数量性状,但粒色则表现出明显丰富的多样性。通过主成分分析,将普通菜豆23个农艺性状归属为7个主成分,初步明确了各主成分代表的农艺性状;通过上述研究,明确了山西普通菜豆主要类型、多样性特征和各主成分代表的主要农艺性状,可为资源鉴定评价提供综合性状指标。     

QTL Mapping of Important Agronomic Traits on Chromosomes Showing Marker Segregation Distortion in Interspecific Backcross Cotton Populations

[Objective] The purpose of this study was to map QTLs for yield, maturity, and fiber quality traits of cotton interspecific hybrid populations and to mine favorable alleles from the cotton genome for QTL fine mapping, gene cloning, and molecular marker-assisted selection. [Method] Four BC₁F₁ populations, E(E3), E(3E), (E3)E, and (3E)E, were developed by crossing sea island cotton 3-79 ("3") and upland cotton 'Emian 22' ("E"), and with "Emian 22" as the recurrent parent. BC1F2 populations derived from BC1F1 populations were phenotyped to allow mapping of QTLs for yield-, maturity-, and fiber quality-related traits on chromosomes showing segregation distortion. [Result] A normal distribution test indicated that six fiber quality-related traits, except for the micronaire value in E (E3) and the short fiber ratio in all populations, followed a normal distribution pattern; in contrast, earliness-related traits were not normally distributed in all populations, which suggests that genotype-environment interactions have strong effects on maturity-related traits. Using composite interval mapping, 47 QTLs were detected on three chromosomes (2, 16, and 18): 15 in (E3)E, 13 in (3E)E, 12 in E(E3), and 7 in E(3E), with the phenotypic variation explained (PVE) ranging from 8.8%–30.9%. A total of 27 fiber-related QTLs were discovered, with the PVE ranging from 9.0%–30.9%. Five yield-related QTLs (PVE = 8.8%–17.4%) and 13 maturity-related QTLs (PVE = 9.4%–19.4%) were also identified. [Conclusion] Comparison analysis showed that most QTLs in our study were consistent with the MetaQTL, including QTLs for boll weight, fiber length, fiber length uniformity, lint percentage, and micronaire value on chromosome 2; QTLs for fiber length, fiber strength, and fiber length uniformity on chromosome 16; and QTLs for boll weight, date of boll opening, fiber length, fiber strength, fiber length uniformity, and micronaire value on chromosome 18. Several detected QTLs in our study were not found in the MetaQTL, such as QTLs for date of boll opening and lint percentage on chromosome 16 and a QTL for the date from seedling to flowering stages on chromosome 18. These newly identified QTLs may provide novel insights for cotton QTL analysis.     

长江中游地区水旱复种轮作模式资源利用率比较研究

为了实现农田资源高效利用,维护农业生态良性循环,优化长江中游地区传统种植模式,本研究于2013年10月—2015年11月,以冬闲连作为对照,分析比较了不同水旱复种轮作模式(冬闲-早稻-晚稻→冬闲-早稻-晚稻、马铃薯-玉米‖大豆-晚稻→蔬菜-花生‖玉米-晚稻、蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻、绿肥-早稻-晚稻→油菜-花生-晚稻、油菜-花生-晚稻→马铃薯-玉米‖大豆-晚稻)的光、温、水、土资源利用效率。结果表明:水旱复种轮作模式的冬季、晚季和周年的光能利用率均比冬闲连作处理高,周年光能利用率两年间分别高8.26%~82.50%和2.63%~121.42%,其中均以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式最高。水旱复种轮作模式的年有效积温利用率均高于冬闲连作模式,两年间分别高12.87%~21.26%和11.17%~25.88%,以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式最佳。两年间水旱复种轮作模式的冬季、晚季和周年的水分利用率均比冬闲连作处理高,其中周年水分利用率分别高45.36%~83.50%和40.00%~118.75%,以"马铃薯-玉米‖大豆-晚稻→蔬菜-花生‖玉米-晚稻"轮作模式的晚季和周年水分利用率最高,"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"模式的冬季水分利用率最高。两年间土地利用率均以"绿肥-早稻-晚稻"复种模式最高,平均利用率达96.11%,"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"轮作模式的土地利用率表现最好。因此,各水旱复种轮作模式的周年光能利用率、年有效积温利用率、水分利用率和土地利用率均高于冬闲对照,其中以"蔬菜-花生‖玉米-晚稻→绿肥-早稻-晚稻"和"绿肥-早稻-晚稻→油菜-花生-晚稻"轮作模式表现较好,适宜在长江中游地区推广应用。     

土施钾肥对骏枣叶片光合特性及果实品质的影响

以新疆阿拉尔垦区直播密植栽培模式下的4年生骏枣为试验材料,研究了密植枣园硫酸钾不同施用量和施肥时期对骏枣叶片光合特性及果实品质和产量的影响。结果表明,土施钾肥显著提高叶片叶绿素含量和净光合速率(Pn),以果实膨大期或坐果期结合膨大期土壤追施硫酸钾450 kg/hm~2处理的效果最为显著,而坐果期施钾肥显著降低骏枣叶片气孔导度(Gs)和蒸腾速率(Tr)。骏枣叶片水分利用效率(WUE)随着施钾量的增加而提高,且施肥时期越早效果越显著;蒸腾速率随施钾量的增加而降低,胞间二氧化碳(Ci)含量未受钾肥调控影响。增施钾肥显著提高了骏枣果实单果重、可溶性糖、淀粉及Vc含量,降低有机酸含量,从而提高糖酸比,并提高单果重和鲜果产量,以果实膨大期土施钾肥450 kg/hm~2效果最为显著,其次为果实硬核期施肥。     

紫云英施用量对土壤活性有机碳和碳转化酶活性的影响

利用紫云英还田的定位试验研究不同紫云英施用量下土壤活性有机碳含量和碳转化酶活性的变化。试验包括对照、施用化肥和4个紫云英施用量(30、60、90和120 t·hm~(-2))处理。结果表明:与对照相比,施用化肥和紫云英均能够显著增加土壤活性有机碳(可溶性有机碳、热水提取态有机碳、微生物生物量碳和颗粒有机碳)含量,并且随着紫云英施用量的增加土壤活性有机碳含量显著增加。土壤碳转化酶活性的变化规律与土壤活性有机碳相同,其中施用化肥和紫云英较对照处理分别提高14%和24%~55%的纤维素酶活性、27%和45%~187%的蔗糖酶活性、42%和51%~165%的β-葡萄糖苷酶活性、11%和11%~24%的酚氧化酶活性以及16%和16%~27%的过氧化物酶活性。与对照相比,施用化肥对相对酶活性(酶活性与微生物生物量碳的比值)无显著影响;增加紫云英施用量能够显著增加相对蔗糖酶和相对β-葡萄糖苷酶活性,降低相对酚氧化酶活性和相对过氧化物酶活性。逐步回归分析表明土壤活性有机碳组分含量主要受纤维素酶、β-葡萄糖苷酶和酚氧化酶活性的影响。     

超低温对降香种子遗传变异和生理特性的影响

采用单因素随机区组试验方案探究降香种子玻璃化超低温保存最适条件,结合显微切片观察和生理生化特性检测对冷冻后细胞形态和生理生化指标变化进行分析,同时运用形态观察法和相关序列扩增多态性（SRAP）分子标记法对超低温保存后的降香植株遗传稳定性进行检测。结果表明：降香种子玻璃化超低温保存最佳条件为：室温（25 ℃）装载 25 min,0 ℃ 玻璃化脱水 30 min,40 ℃水浴解冻 5 min。对比检测不同冷冻时间的降香种子超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、α-淀粉酶、脱氢酶活性、丙二醛（MDA）含量以及电导率等生理生化指标,无明显差异。显微观察超低温保存前后的降香种胚内部结构,未见明显变化。超低温保存后的降香种子发芽时,出现短暂的生长停滞期,恢复培养后,其表观形态指标与对照植株无明显差异。SRAP技术分析了超低温冷冻前后的降香再生植株,其 SRAP 带型基本一致,未见明显差异带型。综上表明,超低温保存降香种子是长期稳定、安全可靠的。     

新麦草种子产量与繁殖性状的关联性分析

为了解新麦草的种子产量及繁殖性状间的关系,对27份新麦草种质资源的单株种子产量与7个产量性状的关联性进行了分析。结果表明,新麦草的单株生殖枝数、穗轴节数、单穗小花数和千粒重与单株种子产量关联最密切,其相关性表现为单株生殖枝数＞千粒重＞穗轴节数＞单穗小花数。经通径分析,单株生殖枝数（X₁）对单株种子产量（Y）直接效应最大,其余依次是单穗小花数（X₄）、单穗小穗数（X₃）、千粒重（X₇）、穗轴节数（X₂）和单穗结实种子数（X₅）,其中单穗结实种子数和结实率对种子产量均表现为负效应,且未达到显著水平。经逐步回归分析得到回归方程Y= -9.880 2+0.27X₁+0.012X₄+2.011 1X₇（R=0.805 8,P<0.001）,说明提高单株生殖枝数、千粒重、单穗小花数对新麦草增产的贡献最大,增加结实率也可间接提高新麦草的单株种子产量。     

Relationships among the chalkiness,kernel size and endosperm cell morphology of rice kernels at different spikelet positions within a panicle

The occurrence of chalky kernels in rice is causally related to kernel size and endosperm morphology. This study aimed to investigate the occurrence of chalky kernels by analyzing kernel size, and the number and area of endosperm cells. Spikelets were sampled from upper and middle primary branches and lower secondary branches in a panicle, and divided into four categories: upper, middle, and lower perfect (PF) kernels and lower milky-white (MW) kernels. On the lower secondary branches, there was a higher percentage of chalky kernels, with smaller kernel lengths, widths and thicknesses, than the kernels on the upper and middle primary branches. MW kernels were smaller in size than PF kernels even on the same lower secondary branches. Regardless of grain appearance quality traits, the total areas of endosperm cross sections in lower kernels were significantly smaller than in upper kernels owing to the decreased cell area, and there was a significant negative correlation between the number of cells and average cell area. When the numbers and the areas of cells were analyzed using angular 30° intervals from the line connecting the center point and the dorsal vascular bundle, the MW kernels had significantly less cells than PF kernels near the ventral side at 120–180°. Thus, the decrease in the number of cells near the ventral side was a main causal factor in the decrease in MW kernel widths compared with PF kernel widths, and this suggested that cell division in MW kernels was inhibited at the early grain-filling stage.