玉米杂交种丹玉403号选育及栽培技术要点

丹玉403号是丹东农业科学院以自选系D95为母本,自选系DH34为父本组配成的玉米单交种,该杂交种抗病、抗倒、高产、稳产,适宜在辽宁、吉林中南部、华北、西北春玉米区种植。     

番茄茉莉酸缺失突变体灰霉菌侵染响应miRNA及其表达分析

为揭示miRNA对番茄灰霉菌胁迫的响应机制,以番茄茉莉酸(JA)缺失突变体def1、spr2及其野生型(CM)为试验材料,构建了3个材料接种灰霉菌前后(0 h、48 h)2个时期的miRNA文库,并采用Illumina平台测序,对测序数据进行生物信息分析,结合实时荧光定量检测目的 miRNA及其预测的靶基因表达情况。结果表明灰霉菌侵染番茄后,JA缺失突变体def1和spr2的病情指数显著高于CM,H₂O₂含量低于CM。高通量测序鉴定了130个已知miRNA和811个新miRNA。进一步筛选出8个保守miRNA(Sly-miR156e-3p、Sly-miR166c-5p、Sly-miR171f、Sly-miR172b、Sly-miR319a、Sly-miR390b-5p、Sly-miR399b、Sly-miR482d-5p),其在6个样本中表达模式各异。预测差异miRNA靶基因122个,结合qRT-PCR技术分析了8个miRNA和8个靶基因的表达情况,与高通量测序结果基本一致。推测Sly-miR156e-3p、Sly-miR390b-5p、Sl...     

Synthetic microRNA‐205 exhibited tumour suppression in spontaneous canine malignant melanoma by intratumoral injection

MicroRNAs (miRNA) are small, noncoding RNA molecules consisting of 18 to 25 nucleotides. Malignant melanomas (MMs) are one of the most common malignancies in both dogs and humans. We previously reported that chemically modified synthetic miRNA‐205 (miR‐205BP/S3) inhibits melanoma growth in vitro and in vivo. The present study aimed to evaluate the efficacy of intratumoral administration of synthetic miR‐205 for spontaneous CMMs and to evaluate its potential as systemic therapy. Ten dogs with various stages of MM were treated with miR‐205BP/S3 injected into tumours. Adverse effects (AEs) were assessed in accordance with the Veterinary Cooperative Oncology Group‐Common Terminology Criteria for Adverse Events (VCOG‐CTCAE) v1.1 guidelines. Five cases attained complete remission (CR), three attained stable disease (SD), and two cases displayed characteristics of progressive disease (PD). In all cases, no changes were observed in the blood parameters upon miRNA administration, and miR‐205BP/S3 administration did not yield any side effects. The present results suggest that intratumoral administration of miR‐205BP/S3 is a potentially applicable treatment for canine melanoma.     

哺乳动物毛囊发育及调控研究进展

毛囊具有高度自我更新能力,是哺乳动物特有的皮肤构造,且是唯一呈终生周期性生长的器官。毛囊的发生始于胚胎期,皮肤上皮层细胞和下胚层细胞间的一系列相互作用诱导形成毛囊,之后毛囊进入周期性循环,包括生长、退行和休止3个阶段。毛囊的发育过程中受到复杂的网络调控。近年来,关于哺乳动物毛囊发育及调控机制的研究取得了较大进展。已有研究表明,毛囊的发生及循环过程中受到多种因子的调控,不同信号通路及miRNA和lncRNA相关基因的参与,构成了一个庞大而又复杂的网络调控图谱,每种调控因子间的相互促进及制约为毛囊的发生及循环提供了必要的保障。文章简述了人、羊及小鼠等哺乳动物毛囊形态发生、周期性循环及相关调控因子的研究进展,为更加全面地了解哺乳动物毛囊发育过程及调控机制提供了参考,同时对人工控制毛绒的周期生长进而提高毛绒产量和质量提供了思路。     

A MicroRNA Catalog of Swine Umbilical Vein Endothelial Cells Identified by Deep Sequencing

MicroRNAs (miRNAs) are endogenous ∼22 nt RNAs that play important regulatory roles in targeting mRNAs for cleavage or translational repression. Despite the discovery of increasing numbers of human and mouse miRNAs, little is known about miRNAs from pig. In this study, we sought to extend the repertoire of porcine small regulatory RNAs using Solexa sequencing. We sequenced a library of small RNAs prepared from immortalized swine umbilical vein endothelial cells (SUVECs). We produced over 13.6 million short sequence reads, of which 8547658 perfectly mapped to the pig genome. A bioinformatics pipeline was used to identify authentic mature miRNA sequences. We identified 154 porcine miRNA genes, among which 146 were conserved across species, and 8 were pig-specific miRNA genes. The 146 miRNA genes encoded 116 conserved mature miRNAs and 66 miRNA*. The 8 pig-specific miRNA genes encoded 4 mature miRNAs. Four potential novel miRNAs were identified. The secondary structures of the 154 miRNA genes were predicted; 13 miRNAs have 2 structures, and miR-9 and miR-199 have 4 and 3 structures, respectively. 36 miRNAs were organized into 19 compact clusters. miR-206, miR-21 and miR-378 were the relatively highly expressed miRNAs. In conclusion, Solexa sequencing allowed the successful discovery of known and novel porcine miRNAs with high accuracy and efficiency. Furthermore, our results supply new data to the somewhat insufficient pig miRBase, and are useful for investigating features of the blood-brain barrier, vascular diseases and inflammation.     

RNA加尾和引物延伸法检测黑腹果蝇3种microRNA表达量

采用RNA加尾和引物延伸real time PCR法实时定量检测了黑腹果蝇Drosophila melanogaster的bantam、mir14、mir-2a共3种microRNA(miRNA)在各发育阶段组织中的相对表达情况,发现3种miRNA在不同发育时期的表达差异明显,其表达变化规律与文献报道基本一致,验证了该法的可靠性.这种"加尾和引物延伸"方法,仅需1~10 ng总RNA或等同物,检测范围可达7个数量级,为昆虫miRNA研究提供了可靠的定量检测方法.     

微小RNA在动物上的功能研究进展*

 微小RNA（miRNA）是一种长约22 nt的非编码RNA,通过与mRNA碱基互补配对来靶基因进行转录后调控。在众多多细胞生物中已经鉴别出数百种miRNAs,而且大多数在进化上高度保守。虽然绝大多数miRNAs的生物学功能还不清楚,但是预测结果显示miRNAs对人类30%的基因具有表达调控作用。随着研究的深入,不断知道miRNAs的功能及其作用机制。本文就microRNAs在动物上的作用机制及其功能的研究进展作一综述。     

德州驴与泌阳驴睾丸组织mRNA与microRNA表达鉴定与分析

试验旨在探究驴睾丸组织中mRNA和microRNA的表达模式，为中国地方驴的转录组研究提供遗传数据。利用RNA-Seq和生物信息学方法分析了中国地方驴睾丸组织表达基因和microRNA，并分析了泌阳驴和德州驴品种间差异表达基因及microRNA。结果表明，mRNA平均clean reads为57837506条，Small RNA平均clean reads为9952015条，分别占原始数据的96.34%和88.86%。在驴睾丸组织中共发现24751个表达基因及1074个表达microRNAs，其中，睾丸组织表达量较高的基因为LOC106836782、XLOC_066401、HSP90AA1、TNP1和COF2，表达量最高的microRNAs为eca-miR-143、eca-miR-21、eca-miR-99a、eca-miR-148a和eca-miR-26a。对泌阳驴与德州驴睾丸组织进行差异表达分析共发现102个差异表达基因及2个差异表达microRNAs，但未富集到功能条目，说明泌阳驴和德州驴品种间差异不明显。本研究提供了驴睾丸组织mRNA及microRNA表达模式和转录组学数据，并鉴定了泌阳驴与德州驴睾丸组织的差异表达mRNA和microRNA，对驴的遗传资源研究具有一定的科学意义。