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991.
如何有效地去除污染区农作物体内持久性有机污染物(POPs),以规避作物POPs污染风险、保障农产品安全,实现污染土壤的资源化利用备受国际关注。植物体内含有内生细菌(EB),其数量和种类丰富,且能重新定殖在目标植物组织的间隙或细胞内部,促进植物生长、提高植物抗逆性,甚至减少植物体内POPs含量。能否利用植物-功能EB的微生态系统,从污染区植物体内获得具有POPs降解特性的功能EB,并将其重新定殖在目标农作物中,进而降低作物体内POPs含量、规避作物污染风险?本文综述了农业生态系统中作物体内POPs的污染现状及植物对POPs的吸收调控作用,系统地评估了具有POPs降解特性的功能EB在目标植物体内的定殖、传导及效能,阐释了功能EB调控植物体内POPs污染的作用机理,展望了功能EB定殖在作物体内调控其POPs污染的优点及仍需解决的技术问题,旨在为利用植物-功能EB的微生态系统规避作物体内POPs污染风险、保障农产品安全和人群健康提供新思路和途径。  相似文献   
992.
多囊卵巢综合症(PCOS)是女性常见的生殖功能障碍性疾病,主要表现为卵巢的雄激素过多及排卵障碍,是引起女性不排卵性不孕的主要原因。PCOS患者主要表现为胰岛素抵抗和高胰岛素血症,而PI3K/Akt信号通路是胰岛素发挥生理作用的主要信号通路,并且Akt的下游底物GSK-3是糖原合成的主要调控因子,本文就PI3K/Akt与胰岛素抵抗的关系做一综述,并对PCOS的治疗进行了展望。  相似文献   
993.
994.
To understand the mechanisms involved in biological control of Dutch elm disease byPseudomonas, data were needed on the distribution of the introduced bacteria within elm and on the development of the bacterial population over a period of time.As traditional biochemical identification techniques are not suitable for distinguishment between individualPseudomonas isolates, three alternative approaches were compared.
1)  Chemotaxonomy, using lipopolysaccharide pattern, cell envelope protein pattern or DNA restriction fragment pattern. These techniques were reliable, but tedious.
2)  Labeling bacteria with a transposon (Tn903) or a plasmid construct (pMON5003) with a metabolic marker (Lac ZY, coding for -galactosidase and lactose permease) allowed for a reliable identification of reisolates. However, populations of transposon-labeled bacteria in elms declined much faster than populations of the unlabeled wild type. The plasmid carrying the metabolic marker disappeared from the bacterial populations over time. Apparently both the transposon and the plasmid were a disadvantage to the bacteria compared with the wild type parent strains.
3)  Immunoagglutination of representative reisolates with an antiserum against theP. fluorescens isolate in use proved to be specific and fast. For routine purposes the immunoagglutination test therefore was the best method of the various ones employed.
  相似文献   
995.
本文论述喂饲草、秸秆或其他残渣时黄牛、水牛的奶肉役力成绩。作者认为,本生产方式是发展广西养牛的一条有效途径,并介绍了适合广西环境条件栽培的国外优良牧草,如象草、银合欢等的特性及饲草价值。  相似文献   
996.
Sugarcane yellow leaf virus (ScYLV) is distributed worldwide and has been shown to be the cause of the disease sugarcane yellow leaf syndrome (YLS). This study was an investigation of the transmission and spread of ScYLV in Hawaii. Several aphids are known to transmit the virus, but investigation of infestation and transmission efficiency showed Melanaphis sacchari to be the only vector important for field spread of the disease. The initial multiplication of ScYLV in a virus-free plant occurred exclusively in very young sink tissues. When a single leaf was inoculated on a plant, that leaf and all older leaves remained virus-free, based on tissue-blot immunoassay, whereas meristems and all subsequently formed new leaves became infected. Therefore, only after those leaves which had already developed before inoculation had been shed, did the complete plant contain ScYLV. Spread of the viral infection to neighbouring plants in the plantation fields via aphids was relatively slow and in the range of a few metres per year. No indication of long-distance transfer could be seen. This indicates that it may be possible to produce and use virus-free seed cane for planting of high-yielding but YLS-susceptible cultivars.  相似文献   
997.
    
A 32-year-old woman attempted suicide by ingesting Gloriosa bulbs and died approximately 2 days later. Toxicological examination revealed a potentially fatal blood concentration of colchicine (0.096 mg/L). In addition to the increased mitotic figures in the gastrointestinal mucosa, a unique finding for acute colchicine intoxication, pathological examination showed microvesicular lipid droplets in the liver, kidney, heart, and conduction system. Furthermore, central chromatolysis of neurons was observed in the pontine nucleus, medial accessory olivary nucleus, nucleus of the solitary tract, and nucleus ambiguus. Grumose degeneration of the cerebellar dentate nucleus was also evident. These pathological findings may help identify colchicine intoxication, even in the absence of evidence suggesting ingestion during autopsy. Moreover, pathological changes in the heart and central nervous system may be associated with the development of serious complications of acute colchicine intoxication.  相似文献   
998.
    
Soil microbial metabolism plays an important role in supporting soil services and functionalities but is usually restricted by the availability of soil nutrients. Although soil microbial metabolism limitation (SMML) has been widely addressed with regard to various driving factors, its response to erosion has not been examined but is urgently needed given the important influence of erosion on global environments. To understand how erosion affects SMML and whether erosion's effect varies with tillage practices and soil types, we conducted a simulated erosion experiment to compare eroded and noneroded plots with contrasting soil types (Mollisol vs. loess soils) and managements (conventional tillage versus no-tillage in plots with crops versus without crops). We measured soil extracellular enzymatic activities and quantified SMML with extracellular enzymatic stoichiometry models. In both soils, the microbes were limited by carbon (C) and phosphorus (P), but not by nitrogen, and microbial C and P limitations were greater in Mollisol than loess soils. For the Mollisol, erosion significantly increased the relative C limitation, whereas the presence of crops eliminated this increase. For the loess soils, erosion enhanced the relative C limitation regardless of crop presence and tillage management. Erosion consistently increased microbial P limitation in plots with crops, with greater effects under no-tillage (+2.9%) compared to conventional tillage (+0.7%) conditions regardless of soil type (p < 0.05). In plots without crops, microbial P limitation was increased by erosion under no-tillage (+2.1%) but was decreased under conventional tillage (−5.6%) (p < 0.05). Additionally, conventional tillage exacerbated microbial C limitation but alleviated microbial P limitation. These results indicated that erosion shifted microbial C and P limitations depending on soil type, tillage practice and the presence of plants, highlighting the importance of considering all these factors when assessing soil microbial metabolic limitation at various spatial scales.  相似文献   
999.
    
Stertor, a clinical sign associated with obstructive airway syndrome, is often observed in non-brachycephalic dogs. This prospective, case-control study aimed to compare soft palate dimensions, nasopharyngeal cross-sectional area (CSA), and nasopharyngeal collapsibility at various locations in non-brachycephalic dogs with and without stertor. A total of 50 dogs were recruited and stratified into control (n = 34) and stertor (n = 13) groups. Static and dynamic computed tomography was conducted without tracheal intubation, and the following variables were calculated: normalized soft palate length and thickness, normalized maximum and minimum nasopharyngeal CSAs (rCSAmax and rCSAmin), and nasopharyngeal collapsibility at the level of the cranial end of the soft palate, pterygoid hamulus, foramen lacerum, bony labyrinth, and caudal end of the soft palate. The stertor group demonstrated significantly lower rCSAmax and rCSAmin, as well as higher nasopharyngeal collapsibility compared with the control group, while no significant differences were noted in the soft palate dimension. Evaluating nasopharyngeal collapse at the foramen lacerum level was recommended due to the clear presence of identifiable bony landmarks and lower overlap in the nasopharyngeal collapsibility between dogs with and without stertor. Physical dimensions of the soft palate may not be the primary contributing factor to nasopharyngeal collapse and clinical signs in non-brachycephalic dogs.  相似文献   
1000.
    
Wheat grain containers or silos can be perfect habitats for insects, which generate large economic losses to grain production. Natural alternatives to synthetic insecticides have grown in popularity because of health, economic and ecological issues. Diatomaceous earth is a natural compound that has an insecticide effect by enhancing an insect's dehydration with no toxicity on mammals including humans. The aim of this study is to confirm the effect of diatomaceous earth as an insecticide for the wheat grain pest, the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) and demonstrate its underlying mechanisms as an insecticide by open-flow respirometry and scanning electron microscopy.  相似文献   
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