Metabolic Profiling and In Vitro Assessment of the Biological Activities of the Ethyl Acetate Extract of Penicillium chrysogenum “Endozoic of Cliona sp. Marine Sponge” from the Red Sea (Egypt)

Marine sponge-derived endozoic fungi have been gaining increasing importance as promising sources of numerous and unique bioactive compounds. This study investigates the phytochemical profile and biological activities of the ethyl acetate extract of Penicillium chrysogenum derived from Cliona sp. sponge. Thirty-six compounds were tentatively identified from P. chrysogenum ethyl acetate extract along with the kojic acid (KA) isolation. The UPLC-ESI-MS/MS positive ionization mode was used to analyze and identify the extract constituents while 1D and 2D NMR spectroscopy were used for kojic acid (KA) structure confirmation. The antimicrobial, antioxidant, and cytotoxic activities were assessed in vitro. Both the extract and kojic acid showed potent antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with MIC 250 ± 0.82 µg/mL. Interestingly, the extract showed strong antifungal activity against Candida albicans and Cryptococcus neoformans with MIC 93.75 ± 0.55 and 19.53 ± 0.48 µg/mL, respectively. Furthermore, KA showed the same potency against Fusarium oxysporum and Cryptococcus neoformans with MIC 39.06 ± 0.85 and 39.06 ± 0.98 µg/mL, respectively. Ultimately, KA showed strong antioxidant activity with IC₅₀ 33.7 ± 0.8 µg/mL. Moreover, the extract and KA showed strong cytotoxic activity against colon carcinoma (with IC₅₀ 22.6 ± 0.8 and 23.4 ± 1.4 µg/mL, respectively) and human larynx carcinoma (with equal IC₅₀ 30.8 ± 1.3 and ± 2.1 µg/mL, respectively), respectively. The current study represents the first insights into the phytochemical profile and biological properties of P. chrysoenum ethyl acetate extract, which could be a promising source of valuable secondary metabolites with potent biological potentials.     

Approaches to Configuration Determinations of Flexible Marine Natural Products: Advances and Prospects

Flexible marine natural products (MNPs), such as eribulin and bryostatin, play an important role in the development of modern marine drugs. However, due to the multiple chiral centers and geometrical uncertainty of flexible systems, configuration determinations of flexible MNPs face great challenges, which, in turn, have led to obstacles in druggability research. To resolve this issue, the comprehensive use of multiple methods is necessary. Additionally, configuration assignment methods, such as X-ray single-crystal diffraction (crystalline derivatives, crystallization chaperones, and crystalline sponges), NMR-based methods (JBCA and Mosher’s method), circular dichroism-based methods (ECCD and ICD), quantum computational chemistry-based methods (NMR calculations, ECD calculations, and VCD calculations), and chemical transformation-based methods should be summarized. This paper reviews the basic principles, characteristics, and applicability of the methods mentioned above as well as application examples to broaden the research and applications of these methods and to provide a reference for the configuration determinations of flexible MNPs.     

发芽糙米的开发及其技术运用

近年,由于日本人饮食习惯的急速的西方化,过去较少见的癌症、心脏病、糖尿病等疾病日益激增。以附加功能性于大米为目的,2001年开发出了采用浸泡式工艺的发芽糙米,发芽糙米所含GABA对高血压及糖尿病等生活习惯病具有预防效果。通过将富含发芽糙米的功能性成分之一的GABA附加于免淘洗米,开发出功能性免淘洗米(免淘洗GABA米)。利用功能性液化液,制成具有降压等作用的免淘洗功能性包膜米。同时,利用发芽糙米等为功能性原料可开发一系列深加工产品。     

检验用砻谷机研制报告

在"十五"科技攻关计划项目<新型检验用砻谷机、碾米机及便携式米质判定仪开发>课题研究中,成功地解决了实验室检验用砻谷机在稻谷脱壳后谷糙分离效果和易损件的耐用性等关键性问题;设计出科学合理的糙米分离系统,筛选出弹性和硬度均适宜的新型耐磨材料作砻谷机的胶辊材料,从而大大提高了产品易损件的耐用性和稻谷的一次性脱壳率,降低了脱壳过程中产生的糙米破碎率.检验用砻谷机,具有小型轻便、结构简单、噪音小、操作方便、经久耐用等特点,适用于各级实验室的稻谷品质检验.     

温盐环境突变对海蜇幼体生存的影响

为配合2005年辽东湾渔场海蜇人工增殖放流项目,笔者对人工放流过程中,海蜇幼体由于从育苗室人海而引起的温盐环境突变以及在长途运输过程中引起的死亡率进行实验研究,其死亡率直接影响到海蜇的回捕率,进而对辽东湾渔场海蜇的资源量造成影响。实验中发现,在超出海蜇生存最佳盐度范围（20～30）,海蜇在高温（30℃、35℃）条件下,较高盐度组（35、40）较较低盐度组（15、20）的海蜇幼体的生存状态好、成活率高。同时,在低温（10℃、15℃）条件下,较低盐度组（15、20）较较高盐度组（35、40）的海蜇幼体的生存状态好、成活率高。     

长期施肥棕壤有机硫矿化特征及其驱动力

[目的]探究长期不同施肥对土壤有机硫矿化量、动力学特征和酶活性的影响,揭示玉米–大豆轮作体系中棕壤有机硫矿化特征及其主要驱动因子.[方法]沈阳农业大学长期定位试验于1979年建立,为玉米–玉米–大豆(一年一熟)轮作模式.试验设置15个处理,本研究选取了其中7个处理,分别为:CK(不施肥)、N1(低量化学氮肥)、N2(高...     

Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada

Viral haemorrhagic septicaemia virus (VHSV) was isolated from mortalities occurring in populations of mummichog, Fundulus heteroclitus, stickleback, Gasterosteus aculeatus aculeatus, brown trout, Salmo trutta, and striped bass, Morone saxatilis, in New Brunswick and Nova Scotia, Canada. The isolated viral strains produced a cytopathic effect on the epithelioma papillosum cyprini cell line. Serum neutralization indicated the virus was VHSV and sequencing identified the rhabdovirus isolates as the North American strain of VHSV. Phylogenetic analysis indicated that the isolates are closely related and form a distinguishable subgroup of North American type VHSV. To our knowledge, this is the first report of VHSV in mummichog and striped bass.     

From the drawing board to the field: an example for establishing an MPA in Penghu,Taiwan

• 1. In order to promote the establishment of the first marine protected area (MPA) in Taiwan, the conservation values of 12 reef sites in the Penghu Islands were evaluated. The results together with a 6‐year surveillance programme revealed that the Chinwan Inner Bay (CIB) possesses the best coral community in the Penghu Islands with the highest coral cover, high species diversity and habitat types, as well as a high conservation value.
• 2. Utilization of biological resources and socio‐economic factors of CIB were investigated by intensive field surveys and a complete visit‐and‐poll survey respectively. Most of the fishing at CIB is for recreational purposes and is characterized by low investment, low harvest rates, high dependence on weather conditions, and self‐consumption.
• 3. The socio‐economic data showed that there was basically no direct conflict of interests with local communities at two neighbouring villages, and most local residents indicated that they would support the MPA proposal.
• 4. A blueprint for a CIB MPA based on the results of these biological and socio‐economic investigations is proposed. Environmental threats including anchor damage and the predation of Drupella snails need to be ameliorated and monitored through the implementation of appropriate management. The involvement of local communities is key to the success of this MPA and environmental education is recommended to promote public awareness.

Copyright © 2007 John Wiley & Sons, Ltd.     

Movements by wild brown trout in a boreal river: response to habitat and flow contrasts

Abstract  Radio transmitters were implanted in wild brown trout, Salmo trutta L., in the River Måna at low summer water flows ( n  = 18), higher flow in summer ( n  =   20), and variable, peaking flows in autumn ( n  =   20), and tracked two to four times day and night for 4–5 weeks. Individuals were caught and released in a 4-km uniformly channelised section, and in a 4-km natural diverse river section. Substantial individual variation in home range and total movement (924–85 818 m² and 295–7014 m) suggested flexibility to adapt to local environmental conditions. Fish were stationary most of the time (median movement 0 m), but some individuals undertook few and apparently sporadic longer movements, sometimes involving shifts in home range. No consistent diurnal pattern in movements was found. Trout in the uniform habitat section appeared to have larger home ranges and moved more than trout in the natural section. Differences were, however, not statistically significant in most comparisons, due to large individual variation. Similarly, larger home ranges and movements between trials related to higher flow were found, but differences were generally not significant. No consistent effects of sudden, extreme peaking flows on area use or movements by the brown trout were observed.     

几种海藻中溴过氧化物酶的筛选及酶学性质

溴过氧化物酶(BrPOD)是具有特殊功能的过氧化物酶,海藻是其主要来源。对几种中国海域的红藻如角叉菜(Chondrus ocellatus)、龙须菜(Gracilaria sjoestedtii)、珊瑚藻(Corallina officinalis)进行了溴过氧化物酶的藻种筛选,并对酶活较高的珊瑚藻进行溴过氧化物酶分离纯化及性质的研究。通过硫酸铵沉淀、DEAE-cellulose 52离子交换层析、Sephadex G-100凝胶层析等方法,从珊瑚藻中分离得到溴过氧化物酶。对该酶性质研究表明,该酶分子量较大,表观分子量为64 kD;溴化单氯甲酮的最适pH值为6.0;pH在5.0-9.0时酶活性稳定;在30-70℃温度范围内酶活性稳定;Ca2 、Co2 、Cu2 、Mn2 、NaF和EDTA等化合物使溴过氧化物酶活性下降,钒酸盐能提高酶活性。反应动力学实验表明,该酶对Br-、H2O2的Km分别为7.40 mmol/L和96.09μmol/L。