全文获取类型
收费全文 | 16242篇 |
免费 | 1265篇 |
国内免费 | 1196篇 |
专业分类
林业 | 2880篇 |
农学 | 1263篇 |
基础科学 | 53篇 |
1388篇 | |
综合类 | 5562篇 |
农作物 | 992篇 |
水产渔业 | 2242篇 |
畜牧兽医 | 2166篇 |
园艺 | 578篇 |
植物保护 | 1579篇 |
出版年
2024年 | 80篇 |
2023年 | 304篇 |
2022年 | 493篇 |
2021年 | 624篇 |
2020年 | 616篇 |
2019年 | 726篇 |
2018年 | 496篇 |
2017年 | 725篇 |
2016年 | 778篇 |
2015年 | 685篇 |
2014年 | 811篇 |
2013年 | 1001篇 |
2012年 | 1090篇 |
2011年 | 1176篇 |
2010年 | 980篇 |
2009年 | 954篇 |
2008年 | 935篇 |
2007年 | 975篇 |
2006年 | 798篇 |
2005年 | 695篇 |
2004年 | 546篇 |
2003年 | 475篇 |
2002年 | 355篇 |
2001年 | 363篇 |
2000年 | 319篇 |
1999年 | 263篇 |
1998年 | 222篇 |
1997年 | 162篇 |
1996年 | 165篇 |
1995年 | 166篇 |
1994年 | 125篇 |
1993年 | 115篇 |
1992年 | 126篇 |
1991年 | 99篇 |
1990年 | 81篇 |
1989年 | 53篇 |
1988年 | 42篇 |
1987年 | 22篇 |
1986年 | 10篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 6篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1962年 | 3篇 |
1956年 | 3篇 |
1955年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
51.
Effects of area and isolation of woodland patches on herbaceous plant species richness across Great Britain 总被引:5,自引:2,他引:5
Petit Sandrine Griffiths Leila Smart Simon S. Smith Geoff M. Stuart Rick C. Wright Simon M. 《Landscape Ecology》2004,19(5):463-471
Richness of Ancient Woodland Indicator plant species was analysed in 308 woodland patches that were surveyed during the Countryside Survey of Great Britain carried out in 1998. The Countryside Survey recorded vegetation plots and landscape structure in 569 stratified 1 km sample squares and developed a remotely-sensed land cover map of the UK. Using these datasets, we tested the hypothesis that Ancient Woodland Indicator species richness in woodland fragments was limited by patch area, shape and spatial isolation and that woodland patches located in the lowland region of Great Britain would respond differently than those in the upland region. The variation in Ancient Woodland Indicator species richness in the British lowlands (n = 218) was mainly explained by patch area and two measures of connectivity, the length of hedgerows and lines of trees in the 1 km square and the area of woodland within 500 m of the vegetation plot. By contrast, variation in Ancient Woodland Indicator species richness in the British uplands (n = 90) was related to Ellenberg scores of the vegetation communities sampled – a surrogate for habitat quality – and no significant effect of spatial structure was detected. It therefore appears that the degree of fragmentation of woodland in the British lowlands limits the distribution of Ancient Woodland Indicator species, while in the uplands, failed colonisation is a matter of habitat quality rather than a result of landscape structure.This revised version was published online in May 2005 with corrections to the Cover Date. 相似文献
52.
NAD(P)H oxidase was initially found in phagocytes and it participates in the generation of reactive oxygen species(ROS). Recent researches have showed that NAD(P)H oxidase also expresses in other tissues including blood vessels and it plays a critical role in vascular remodeling through ROS which are important signaling molecules in vascular cells.This article reviews the biochemical characterization, activation paradigms, structure, and function of this enzyme. 相似文献
53.
酵母铬与L-肉碱对蛋鸡生产性能及脂质代谢的影响 总被引:6,自引:0,他引:6
选用21周龄海兰褐壳蛋鸡288只,随机分为9组,每组32只,采用3×3(铬×L-肉碱)2因子3水平有重复交叉试验设计,预试2周后,在玉米-豆粕型日粮的基础上添加不同水平的酵母铬(以铬计:0、400、600μg/kg)与L-肉碱(0、50、100mg/kg),以饲喂基础日粮组为对照组,用以研究二者对蛋鸡生产性能及脂质代谢的影响,并初步探讨二者的互作效应,试验期7周。结果表明:600μg/kg铬或100mg/kgL-肉碱对脂质代谢多数指标效果显著(P<0.05或0.01);二者同时添加对蛋黄胆固醇、肝脏甘油三酯及腹脂率等均产生显著互作效应(P=0.0003~0.0500),且400μg/kg铬和100mg/kgL-肉碱混合添加对降低蛋黄胆固醇比其它各组均表现出优势,400μg/kg铬和50mg/kgL-肉碱混合添加对降低腹脂率比其它各组均表现出优势。铬或(与)L-肉碱在降低蛋鸡肝脂、腹脂及蛋黄胆固醇的同时并未影响蛋鸡的生产性能。 相似文献
54.
55.
ABSTRACT1. The objectives of the current study were to investigate the mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken, and provide molecule base to preserve and explore the specific chicken strain.2. Based on sequencing and clustering, the complete mitochondrial DNA map and sequences of Lueyang black-bone chicken were revealed, and two phylogenetic trees of Lueyang black-bone chickens based on D-loop sequences and the mitochondrial genome were constructed.3. The results showed that the complete mitochondrial genome of Lueyang black-bone chickens is 16,784bp in size, consisting of 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region. The base composition of the complete mtDNA sequence is 30.28% for A, 23.78% for T, 32.42% for C, 13.52% for G. Additionally, 10 haplotypes of D-loop sequences in 32 Lueyang black-bone chickens were detected, which were distributed into 4 clades (A, B, C and E).4. It was concluded that genetic diversity is wide in Lueyang black-bone chickens, and this strain has multiple maternal origins from different regions in China and neighbouring regions. 相似文献
56.
Branched‐chain amino acid ratios in low‐protein diets regulate the free amino acid profile and the expression of hepatic fatty acid metabolism‐related genes in growing pigs 下载免费PDF全文
Y. H. Duan F. N. Li C. Y. Wen W. L. Wang Q. P. Guo Y. H. Li Y. L. Yin 《Journal of animal physiology and animal nutrition》2018,102(1):e43-e51
Liver metabolism is affected by nutrients. The aim of this study was to explore the effects of low‐protein diets (17% crude protein, CP) supplemented with branched‐chain amino acids (BCAAs), including leucine (Leu), isoleucine (Ile) and valine (Val), on hepatic amino acid profile and lipid metabolism in growing pigs. The ratio of Leu : Ile : Val in all groups was 1 : 0.51 : 0.63 (20% crude protein, CP), 1 : 1 : 1 (17% CP), 1 : 0.75 : 0.75 (17% CP), 1 : 0.51 : 0.63 (17% CP) and 1 : 0.25 : 0.25 (17% CP) respectively. Results revealed that compared to the positive control group (1 : 0.51 : 0.63, 20% CP), the low‐protein diets significantly augmented the concentrations of most essential amino acids and non‐essential amino acids (p < .05), with the greatest values observed in the 1 : 0.25 : 0.25 group. Moreover, relative to the control, the low‐protein diets with the Leu : Ile : Val ratio ranging from 1 : 0.75 : 0.75 to 1 : 0.25 : 0.25 markedly downregulated the mRNA abundance of acetyl‐CoA carboxylase (ACC), lipoprotein lipase (LPL) and fatty acid‐binding protein 4 (FABP‐4) (p < .05), and upregulated the mRNA expression of hormone‐sensitive lipase (HSL), peroxisome proliferator‐activated receptor‐g coactivator‐1α (PGC‐1α), uncoupling protein 3 (UCP3) and liver carnitine palmitoyltransferase 1 (L‐CPT‐1) (p < .05). Therefore, our data suggest that protein‐restricted diets supplemented with optimal BCAA ratio, that is, 1 : 0.75 : 0.75–1 : 0.25 : 0.25, induce a shift from fatty acid synthesis to fatty acid oxidation in the liver of growing pigs. These effects may be associated with increased mitochondrial biogenesis. 相似文献
57.
Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts 下载免费PDF全文
TC Marques EC da Silva Santos TO Diesel LO Leme CF Martins MAN Dode BG Alves FPH Costa EB de Oliveira ML Gambarini 《Reproduction in domestic animals》2018,53(1):226-236
Effects of adding different concentrations of melatonin (10?7, 10?9 and 10?11 M) to maturation (Experiment 1; Control, IVM + 10?7, IVM + 10?9, IVM + 10?11) and culture media (Experiment 2; Control, IVC + 10?7, IVC + 10?9, IVC + 10?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10?9, IVC + 10?9, IVM /IVC + 10?9). In Experiment 1, maturated oocytes from Control and IVM + 10?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10?7 (43.5%; 56.7%) and IVC + 10?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC + 10?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM + 10?9. Reactive oxygen species production was greater in the IVM /IVC + 10?9 treatment group. In conclusion, the 10?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality. 相似文献
58.
59.
Min Zhang Dan Zheng Zhimei Peng Yuting Zhu Rongrong Li Qiong Wu Yan Li Hongyi Li Weihua Xu Mao Zhang Qichun Hung Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131
In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers. 相似文献
60.
S.‐E. Jacobsen 《Journal of Agronomy and Crop Science》2011,197(5):390-399
In Bolivia, one of the world’s most important centres of plant domestication, there is growing awareness of the value of native Andean crops, both for domestic consumption and for market sale – notably the virtually boom‐like consumer demand for quinoa around the world. The southern altiplano of Bolivia, south of Oruro, relies almost purely on the production of quinoa and breeding of llamas, which have also been selected as the two commodities of priority to the government to increase the income of the country. Presently, however, quinoa is facing increasing problems in production, owing to its increasing export market and price. The flat areas around the salt desert of the southern altiplano, previously characterized by natural vegetation fed by the llamas, are being increasingly sown with quinoa, hence transformed into deserts, because intensive cultivation methods make the soil loose its fertility. Possible solutions to these problems will require extensive efforts in the south, in addition to various strategies, which also include other parts of the Bolivian altiplano and a strengthened focus on other Andean crops. 相似文献