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21.
22.
Yoshiaki Fujita Keita Sunaga Su‐Hyeun Shim Wataru Yamada Takayuki Ohnishi Sang Woo Bang 《Plant Breeding》2018,137(2):162-170
In Brassica oleracea, production of F1 hybrid seeds mainly makes use of the improved Ogura cytoplasmic male sterile (CMS) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferred Diplotaxis erucoides cytoplasm to B. oleracea cultivars using an alloplasmic B. rapaCMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The new B. oleraceaCMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, the B. oleraceaCMS lines carrying D. erucoides cytoplasm had larger leaf areas and a normal plastochron. As a consequence, the B. oleraceaCMS lines carrying D. erucoides cytoplasm have the potential to be valuable alternatives for use in commercial B. oleracea hybrid seed production. 相似文献
23.
Parasitism by parasitoid wasps on the larvae of Epinotia granitalis (Butler), which is a wood-injuring pest in coniferous plantations, was observed for 6 years in two plantation forests of
Chamaecyparis obtusa (Sieb. et Zucc.) Endl. and Cryptomeria japonica (L.f.) D. Don that were adjacent to each other and consisted of trees of almost the same size. E. granitalis larvae were collected from early April to mid-May, then reared in glass bottles, and finally examined to determine whether
or not the larvae had been parasitized. Five species of parasitoids were recorded; two of them were classified in Ichneumonidae,
Campoplex spp. A and B, and others in Braconidae, Iconella repleta Papp, Bassus cingulipes Nees von Esenbeck, and Bracon spp. In all 6 years, parasitism was first observed around 20 April. Species composition of parasitoids differed greatly among
years and between forests. In 1 year in each forest the percentage parasitism increased significantly with the sampling date,
indicating that not all parasitoids attack the same developmental stage of E. granitalis. The mean percentage parasitism per year was significantly higher in the forest of C. obtusa [11.5(%) ± 5.7(SD)] than in the forest of C. japonica (3.0 ± 4.1). These results suggest that the efficiency of parasitoid adults in finding hosts differed between the two forests.
Received: September 20, 2001 / Accepted: August 5, 2002
Acknowledgments We are grateful to Dr. K. Maeto, Shikoku Research Center, Forestry and Forest Products Research Institute, and to Dr. K.
Konishi, National Agricultural Research Center for Hokkaido Region, for identifying parasitoids, and to Dr. T. Miyaura, Forest
Tree Breeding Center, and Dr. H. Kosaka, Forestry and Forest Products Research Institute, for their helpful advice throughout
the study.
Correspondence to:K. Kato 相似文献
24.
不同温度下两种管材应力应变特性的研究 总被引:1,自引:0,他引:1
通过对国产X60螺旋埋弧焊管(SSAW)和进口X52直缝电阻焊管(ERW)的温度拉伸试验,研究了两种钢管母材和焊缝的应力应变特性。试验结果表明,两种焊管的屈服强度和抗拉强度随温度的降低而提高,断后伸长率、最大均匀变形断面收缩率、断后断面收缩率以及屈强比随温度降低而降低;两种焊管母材应力应变曲线特征为连续屈服,而焊缝为非连续屈服,存在明显物理屈服现象;X60SSAW焊管母材与焊缝强度基本相当,X52 相似文献
25.
Yohei Koide Leodegario A. Ebron Hiroshi KatoHiroshi Tsunematsu Mary Jeanie Telebanco-YanoriaNobuya Kobayashi Masao YokooSachio Maruyama Tokio ImbeYoshimichi Fukuta 《Field Crops Research》2011,123(1):19-27
A set of near-isogenic lines for blast resistance genes was developed by using an Indica-type elite rice variety, IR49830-7-1-2-2, suitable for the rainfed lowland conditions in the tropics, as a genetic background. Initially, we revealed that IR49830-7-1-2-2 harbors five blast resistance genes - Pia, Pib, Pik-s, Pita, and Pi11(t) - by using a differential system involving 19 selected standard blast isolates from the Philippines. Based on this result, we developed nine near-isogenic lines (NILs) targeting eight resistance genes - Pik, Pi7(t), Pi3, Pi5, Pita-2, Piz-5, Pish, and Pi9 - by recurrent backcrossing. The introgression of each resistance gene in the NILs was confirmed by reaction patterns to the blast isolates, allelism tests, and DNA marker analysis. In addition, a genome-wide DNA marker survey revealed that most of the chromosome regions in each NIL were of the IR49830-7-1-2-2 type. The agricultural characteristics of most of the developed NILs were almost the same as those of IR49830-7-1-2-2. Moreover, with one exception, they showed submergence tolerance similar to IR49830-7-1-2-2. The developed NILs could be used as a multiline variety suitable for the rainfed lowland in the tropics. 相似文献
26.
27.
Toby A. Joyce Michael T. Abberton T.P.T. Michaelson-Yeates John W. Forster 《Euphytica》1999,107(3):159-165
The RAPD-PCR technique has been used to generate DNA amplification profiles from a set of advanced generation inbred lines of white clover ( Trifolium repens L.). The genetic distances between lines have been estimated and have been compared with the extent of positive heterosis for dry matter yield in hybrid crosses. The analysis clearly separated the four major groups (H, J, R and S), with little within group variation. Heterosis, previously shown to be positively correlated with the extent of phenotypic variation between parental lines, was greatest between lines from groups showing the least genetic distance. The lack of correlation between heterosis and genetic divergence is discussed in the context of results from other systems. Additionally, the data allow a comparison of the extent of phenotypic variation for a trait under selective pressure with the degree of variation at the DNA level detected with the RAPD technique. This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
28.
T‐C Cheng Y‐S Lai I‐Y Lin C‐P Wu S‐L Chang T‐I Chen M‐S Su 《Journal of fish diseases》2010,33(2):161-169
Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate‐buffered saline containing 0.25% trypsin at 25 °C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 °C in Leibovitz‐15 medium containing 10% foetal bovine serum. The cells have been sub‐cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequences of mitochondrial cytochrome oxidase subunit I genes (cox I) with the cox 1 sequence from cobia muscle tissue. The cell lines showed polyploidy. No mycoplasma contamination was detected. Susceptibility to grouper iridovirus was observed for the CB cell line but not the CF cell line. Both cell lines expressed green fluorescent protein after being transfected with green fluorescent reporter gene driven by the cytomegalovirus promoter. 相似文献
29.
为耐密植玉米新品种的选育提供参考,以玉米Lancaster群5个改良自交系为父本,Reid群和Dom群5个骨干自交系为母本,按NCⅡ设计组配25个玉米杂交组合,研究5.5万株/hm~2、7万株/hm~2和9万株/hm~2种植密度对各杂交组合及亲本单株产量杂种优势及配合力的影响。结果表明:在3种种植密度下,25个玉米杂交组合单株产量的杂种优势分别为137.12%、134.90%和127.86%,且杂种优势随种植密度的增加而降低;但PH4CV改良自交系父本所配杂交组合在高密度种植条件下的杂种优势较高。在5.5万株/hm~2种植密度下,一般配合力以自交系PH5AD最高,为7.02;在7万株/hm~2和9万株/hm~2种植密度下,以自交系PH4CV最高,分别为20.73和18.24。以PH5AD为父本所配杂交组合适宜稀植,以PH4CV为父本所配杂交组合在高密植条件下易获高产;以PH4CV为父本所配杂交组合较耐密植,以J1577为父本所配杂交组合宜稀植。 相似文献
30.
通过对我省湘南低山丘陵区红壤土、石灰土、钙质土立地条件下 ,批杷优良品种丰产栽培技术试验林、示范林的调研及相关资料的系统分析研究 ,提出了枇杷在我省湘南低山丘陵区红壤、石灰岩、钙质页岩地区配套的优质丰产、高效栽培技术体系 :因地整地、合理施肥、疏花疏果、果实套袋、抓好夏季修剪、防治病虫害等 相似文献