蓝刺头花絮水提物的降血糖作用研究

【目的】研究蓝刺头花絮水提物的降血糖作用。【方法】采用煎煮法制备蓝刺头花絮水提物。使用四氧嘧啶诱导糖尿病小鼠模型,并随机分为4组,每组10只,蓝刺头高、中、低剂量组小鼠每天分别灌胃1.00,0.50和0.25g/mL的蓝刺头花絮水提物,对照组灌等量蒸馏水,于试验0,7,14和21d测定糖尿病小鼠空腹血糖、体质量;21d测定小鼠口服糖耐量、肝糖原、血清胰岛素、胰高血糖素及肾上腺素的含量。【结果】蓝刺头花絮水提物能够显著降低糖尿病小鼠的空腹血糖,改善糖尿病小鼠的口服糖耐量,升高肝糖原及血清胰岛素水平,极显著降低血清肾上腺素含量,对体质量和血清胰高血糖素含量无显著影响。【结论】蓝刺头花絮水提物能够降低糖尿病小鼠的血糖水平,且呈剂量依赖关系。     

黑苦荞米黄酮提取工艺优化及其降血糖活性研究

为了获得合适的黑苦荞米黄酮提取工艺,该文采用单因素试验和响应面设计,选择乙醇体积分数、料液比、提取时间、提取温度4个因素,优化黑苦荞米黄酮提取工艺。试验结果表明,黑苦荞米黄酮的最佳提取条件是:乙醇体积分数54%,料液比1:24 g/m L,提取时间62 min,提取温度71℃。在此条件下,理论黄酮得率为2.21%,实际黄酮得率为2.20%,相对误差为0.45%。在此基础上进一步研究了黑苦荞米黄酮的α-淀粉酶抑制活性,结果表明7.5 mg/m L的黑苦荞米黄酮对α-淀粉酶活性的抑制率为54.05%,与二甲双胍(5 mg/m L)效果相当;此外,与空白对照相比,50μg/m L黑苦荞米黄酮能显著(P0.05)提高肝脏细胞Hep G2的葡萄糖消耗量(48.73%),并促进肝脏细胞糖原的合成。研究结果表明,黑苦荞米黄酮具有较好的辅助降血糖功效。     

9 O 小檗碱糖苷系列产物的合成及体内降糖活性初步研究

以常见单糖作为取代基,采用固-液相转移催化法合成了9-O-小檗碱糖苷系列产物,合成化合物分子结构分别通过紫外光谱和核磁共振分析等方法进行了确认.通过对清洁级SD大鼠尾静脉注射四氧嘧啶获得实验性高血糖大鼠模型,分组对大鼠灌胃给药9-O-小檗碱葡萄糖苷、小檗碱与二甲双胍阳性对照药15d,并检测期间空腹血糖值.结果显示9-O-小檗碱葡萄糖苷具有明显体内降血糖能力,给药15d后高血糖大鼠血糖下降超过56%,优于小檗碱.     

茶桑混合袋泡茶降血糖作用的实验研究

对茶桑混合袋泡茶的降血糖作用进行了研究。以低、中、高三个剂量(0.5、1、3g/kg·bw)的袋泡茶饲喂正常小鼠模型、高血糖小鼠模型14d后,给予葡萄糖2.5g/kg·bw灌胃,于0、0.5h和2.0h时相分别测定其空腹血糖(FBG)和耐糖量(GT)。结果表明,在由四氧嘧啶引起的高血糖模型动物糖耐量试验中,中、高剂量组显著降低了0.5h和2h时相血糖含量和0.5~0h时相血糖差值,且不影响正常对照组的血糖、糖耐量、糖耐量差值,表明茶桑混合袋泡茶具有降低血糖的作用。     

Antidiabetic Activity of Differently Regioselective Chitosan Sulfates in Alloxan-Induced Diabetic Rats

The present study investigated and compared the hypoglycemic activity of differently regioselective chitosan sulfates in alloxan-induced diabetic rats. Compared with the normal control rats, significantly higher blood glucose levels were observed in the alloxan-induced diabetic rats. The differently regioselective chitosan sulfates exhibited hypoglycemic activities at different doses and intervals, especially 3-O-sulfochitosan (3-S). The major results are as follows. First, 3,6-di-O-sulfochitosan and 3-O-sulfochitosan exhibited more significant hypoglycemic activities than 2-N-3, 6-di-O-sulfochitosan and 6-O-sulfochitosan. Moreover, 3-S-treated rats showed a more significant reduction of blood glucose levels than those treated by 3,6-di-O-sulfochitosan. These results indicated that –OSO₃⁻ at the C3-position of chitosan is a key active site. Second, 3-S significantly reduced the blood glucose levels and regulated the glucose tolerance effect in the experimental rats. Third, treatment with 3-S significantly increased the plasma insulin levels in the experimental diabetic rats. A noticeable hypoglycemic activity of 3-S in the alloxan-induced diabetic rats was shown. Clinical trials are required in the future to confirm the utility of 3-S.     

低剂量复合螺旋藻多糖降血糖作用研究

通过动物试验来观察低剂量复合螺旋藻多糖的降血糖功能。通过腹腔注射四氧嘧啶建立糖尿病小鼠模型,灌胃不同成分的低剂量复合螺旋藻多糖。30d后测量血糖值、肝脏SOD酶活性、免疫器官指数、负荷葡萄糖耐量试验以及肝糖原的含量。通过数据分析对结果进行判断。结果:复合组PSPGBE=21的降血糖作用优于其他组分。结论:低剂量复合螺旋藻多糖具有一定的降血糖功能。     

獐牙菜属药用植物化学成分研究

报道了从獐牙菜属植物中提取的具有降血糖活性成分的化合物 ,并用光谱方法对其结构进行了鉴定     

减压耦合超声法提取桑叶脱氧野尻霉素及降血糖活性研究

[目的]为高效制备桑叶中的一种多羟基生物碱—脱氧野尻霉素(Deoxynojirimycin,DNJ),以期为建立桑叶DNJ提取新技术及其营养价值的开发提供参照.[方法]以桑叶为原料,采用响应面优化法建立基于减压耦合超声法提取桑叶DNJ的工艺条件优化,并以其对α-葡萄糖苷酶与蔗糖酶的综合抑制率为指标,考察其与降血糖活性间...     

α-葡萄糖苷酶抑制剂的研究进展及食品源抑制剂的开发前景

随着世界上糖尿病患者的不断增加,除了传统的药物治疗以外,如今糖尿病及营养学专家更希望患者添加对辅助降血糖食品的应用,并建议用食疗的方法调节血糖水平,有效预防糖尿病的产生。在众多辅助降糖保健食品中,糖类物质、多酚类、黄酮类、磷脂酸类、生物碱等作为α-葡萄糖苷酶活性抑制剂,在降血糖方面起着极其重要的作用。     

Cortical laminar necrosis detected by diffusion-weighted imaging in a dog suspected of having hypoglycemic encephalopathy

We describe a 5-year-old castrated male dog suspected hypoglycemic encephalopathy that was evaluated by using diffusion-weighted imaging (DWI). The dog experienced hypoglycemia after prolonged generalized and continued partial seizures. In the acute phase, DWI showed hyperintensity in the left temporal lobe. After about a month, DWI maintained hyperintensity, and left middle cerebral artery dilation was noted on magnetic resonance angiography (MRA). In the chronic phase, the left temporal lobe lesion was replaced by cerebrospinal fluid. In humans, it was reported that cortical laminar necrosis (CLN) with hypoglycemic encephalopathy presents hyperintensity in the cerebral cortex on DWI and increased vascularity of the middle cerebral artery branches on MRA. In conclusion, DWI has detected CLN in a dog suspected hypoglycemic encephalopathy.