hrpZ_(Psta)在转基因大豆中定量表达与疫霉根腐病和灰斑病抗性相关研究

以T5转hrpZPsta基因大豆JN29-705-15和JL30-187为材料,利用实时荧光定量PCR技术(Quantitative Real Time PCR,qRT-PCR)检测了目标基因在转基因大豆不同组织中的表达量,分别利用下胚轴侵染法和叶面喷施法鉴定疫霉根腐病抗性和灰斑病抗性,并分析目的基因表达量与疫霉根腐病和灰斑病抗性的相关性。结果表明:hrpZPsta基因在大豆的叶、茎、根、籽粒中均有表达,二个株系平均相对表达量分别为8.2/6.1、0.9/0.7、6.5/4.6和0.8/0.7;T5转hrpZPsta基因大豆抗疫霉根腐病和灰斑病能力与野生型相比均有所提高,JN29-705-15对疫霉根腐病抗性从感病提高到中抗,而JL30-187从中抗提高到抗病;hrpZPsta基因在叶中的表达量也与抗灰斑病能力呈正相关,与病情级别呈极显著负相关。试验结果初步证明了外源基因hrp ZPsta在大豆植株中的表达量与受体植株对疫霉根腐病和灰斑病抗性存在一定的相关性。     

防洪堤施工中筑堤粗粒土控制干密度的合理性分析

文章对相对密度控制问题进行分析,并提出几点建议。     

深两优5814作烟后稻旱育抛秧高产栽培技术

深两优5814系国家杂交水稻工程技术研究中心深圳清华龙岗研究所用"Y58S"与自选"恢复系丙4114"组配选育的优质、高产、抗病的两系超级杂交水稻新品种,属弱感光型。2009年宁化县引进种植,根据其特征特性并结合宁化县的气候条件,总结深两优5814作烟后稻旱育抛秧高产栽培技术。     

Associations between postpartum diseases and milk yield and changes in body condition between drying off and parturition of dairy cows in Argentina

ABSTRACT

Aims: To assess the change in body condition score (BCS) during the early and late dry periods and its association with postpartum diseases and milk yield in grazing dairy cows from central Argentina.

Methods: BCS assessments during the dry period, and cow health and milk production records up to 90 days in milk (DIM), were collated for cows from 28 farms at monthly visits between 2007 and 2008. Cows were categorised into four groups; those in Group 1 (n=7,067) maintained or gained BCS during the early and late dry periods; Group 2 (n=2,615) maintained or gained BCS during the early dry period and lost BCS during the late dry period; Group 3 (n=1,989) lost BCS during the early dry period and maintained or gained BCS during the late dry period; and Group 4 (n=5,144) lost BCS during the early and late dry periods.

Results: Cows in Group 1 had reduced odds of having retained fetal membranes (RFM), metritis, and clinical mastitis up to 90 DIM than cows in Group 2 (p<0.001), but the odds of disease were similar to cows in Group 3. The odds of having RFM or clinical mastitis tended to be lower in cows in Group 1 than cows in Group 4 (p=0.08). The odds of cows being culled or dying during the first 90 DIM were lower for cows in Group 1 than for those in Groups 2, 3, and 4 (p≤0.05). Mean accumulated milk yield up to 90 DIM was higher in cows in Group 1 than Group 2 and Group 4 (p<0.001), but was similar to that of cows in Group 3 (p=0.28).

Conclusions and clinical relevance: Cows that lost BCS during the late dry period had increased odds of being diagnosed with several postpartum diseases and had decreased milk yield compared to cows that maintained or gained BCS during the entire dry period. Loss of BCS during any stage of the dry period was also associated with increased incidence of culling or death during the first 90 DIM. These results should raise awareness among dairy cattle producers of the importance of properly managing cow body condition during the dry period, especially during the late dry period.     

核桃黑斑病综合防治技术

通过对核桃黑斑病发病侵染规律及原因分析,采取加强水肥土管理,合理修剪,减少密蔽,改善通风透光条件;谨防蛀果害虫;越冬管理;药剂防治的综合措施防治,可有效控制核桃黑斑病的发生危害。     

不同沟垄覆盖下土壤水热效应对旱作马铃薯生长及产量的影响

针对宁南山区春季降雨少、低温不利于马铃薯出苗,而作物生育中后期高温胁迫限制马铃薯块茎形成导致减产等问题,于2016年设置垄覆地膜沟内覆盖塑料地膜(DM)、玉米秸秆(JG)、生物降解膜(SM)、麻纤维地膜(MM)、液态地膜(YM)及沟不覆盖(BM),以传统平作为对照(CK),研究沟垄覆盖模式对土壤温度、土壤水分、旱作马铃薯生长及块茎产量的影响.结果表明:处理DM,SM,MM和YM马铃薯生育期耕层土壤温度表现出增温效应,而处理JG和BM表现为降温效应,其中处理DM增温效果和处理JG降温效果最佳.处理YM,JG对马铃薯生长前期保水效果较好,分别较CK显著提高13.3%,27.0%,而处理JG,DM对生育中后期的保墒效果较佳,分别较CK显著提高22.4%,13.2%.处理JG可显著促进马铃薯生长,其增产效果最为显著,DM次之,分别较CK增产47.8%,44.8%,其他处理较CK差异不具有统计学意义.可见,在宁南山区实施垄覆地膜沟覆秸秆模式可调控土壤水热环境,对旱作马铃薯生长及增产效果最佳.     

仿生法评定抗草甘膦玉米和转Bt基因玉米的酶水解物能值的研究

本试验旨在分析抗草甘膦玉米和转Bt基因玉米原料及饲粮与同源非转基因玉米原料及饲粮体外总能消化率以及酶水解物能值,为转基因玉米的营养实质等同性仿生评定方法的研究提供参考。试验采用单因素完全随机设计,使用单胃动物仿生消化系统模拟饲料原料和饲粮在鸡胃肠道的消化过程,分析同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应的3种玉米-豆粕饲粮在不同体外模拟消化阶段的干物质消化率、总能消化率和酶水解物能值的差异。结果表明:同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应饲粮在常规概率成分含量上是相似的。抗草甘膦玉米及饲粮与同源非转基因玉米及饲粮相比,在干物质和能量胃消化率、全消化道消化率及酶水解物能值上均没有显著差异(P0.05)。转Bt基因玉米全消化道总能消化率低于同源非转基因玉米(P=0.03,变异系数=0.50%),对应玉米饲粮的酶水解物能值则高于同源非转基因玉米饲粮(P=0.02,变异系数=1.12%),但均处于仿生消化系统测试的误差范围内(变异系数≤1.64%)。由此可见,抗草甘膦玉米的酶水解物能值与同源对照玉米没有差异,而转Bt基因玉米存在统计学意义上的差异,但所有的测值均处于仿生消化系统的测试误差之内。仿生法发现的差异是否具有生物学意义有待体内试验验证。仿生法可为转基因饲料营养等同性研究提供一种新方法。     

油茶高接换冠枯桩现象调查与研究

为了探寻影响油茶高接换冠后枯桩的相关因素,为今后油茶高接换冠技术改良提供理论依据,调查研究了嫁接方法、接穗品种、砧木粗度、留截高度和嫁接方位等因素对油茶高接换冠后枯桩现象的影响。结果表明:5种因素对油茶枯桩程度影响极显著。其中,采用插皮腹接、砧木直径2~6 cm枯桩程度最低;采用‘华硕’和‘湘林XLJ14’其枯桩程度显著低于其它品种;留截高度越低枯桩程度越低;嫁接方位在外侧枯桩程度最低。偏相关分析结果表明,留截高度对油茶枯桩程度的影响最大;其次是嫁接方法、砧木直径和嫁接品种;而嫁接方位对油茶枯桩程度的影响最小。     

Economic performance and comparative riskiness of different management practices for control of botrytis fruit rot in florida strawberry

Florida strawberry farmers usually rely on weekly calendar fungicide applications to prevent yield loss from Botrytis fruit rot (BFR). The Strawberry Advisory System (SAS), which helps optimize the timing of fungicide applications, is an additional tool in the farmers' arsenal of disease control options. This study uses data from two-year production trials in Florida combined with historical strawberry prices to measure the economic performance of the Calendar and SAS-based BFR management practices under output price risk and producers' risk preferences. We use Monte Carlo simulation to model the effect of the variability in weekly output prices on farm returns. Results reveal that the traditional calendar-based system is not the least risky BFR management option as growers expected to be. SAS-based options such as SAS-Switch and SAS-Luna would be preferred by both risk-neutral and risk-averse producers and are recommended under high disease pressure conditions.     

中国荷斯坦牛干奶期长度的群体特征及其与生产性能的关系

本研究旨在探究北京地区中国荷斯坦牛干奶期长度（DPL）的变化规律及其变异对奶牛各项生产性能的影响，包括产奶性能、繁殖性能、初乳品质和乳房健康。收集了北京地区34个牧场出生于2009—2018年136 231头中国荷斯坦牛的干奶日期、生产性能记录、繁殖性能记录等。通过整理试验牛群的干奶记录，揭示了北京地区中国荷斯坦牛1胎和2胎DPL的群体规律，利用固定模型分析了胎次、产犊季节、场-产犊年和初产月龄对DPL的影响；此外，利用固定模型分析了DPL的变异对日产奶量、乳脂率、乳蛋白率、首末次配种间隔（interval from first to last insemination，IFL）、产犊至首次配种间隔（interval from calving to first insemination，ICF）和初乳品质的影响，利用Logistic回归过程分析了DPL的变异对首次配种受胎率（conception rate of first insemination，CR）、首次配种56 d不返情率（56-days non-return rate of first insemination，NRR56）、难产率和乳房健康的影响。研究结果显示，北京地区中国荷斯坦牛DPL平均为58.81 d，胎次、产犊季节、场-产犊年和初产月龄均对DPL有显著影响（P<0.05）。DPL的变异对日产奶量、乳蛋白率、ICF、CR、NRR56、难产率和乳房健康有显著影响（P<0.05）。干奶期短的奶牛测定日平均乳蛋白率、CR和NRR56更高；DPL适中的奶牛测定日平均产奶量更高、乳房健康水平更好、ICF更短、难产率更低。本研究在国内利用大规模牛群数据揭示了中国荷斯坦牛DPL的群体规律，可为中国规模化奶牛场提升管理水平提供参考。