Isolation and Identification of BPIV3 Genotype C

To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺ showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.     

氮磷钾配施对红花产量及其有效成分在器官中分配的影响

于2018—2019年在河南科技学院试验田,以百农红花1号为材料,设计氮、磷、钾三因素和无肥(N0、P0、K0)、低肥(N1、P1、K1)、中肥(N2、P2、K2)、高肥(N3、P3、K3)四水平试验,以T1(N0P0K0)为对照,T2 (N0P2K2)、T3(N1P2K2)、T4(N2P2K2)、T5(N3P2K2)、T6(N2P0K2)、T7(N2P1K2)、T8(N2P3K2)、T9(N2P2K0)、T10(N2P2K1)、T11(N2P2K3)为不同配施水平,共11个处理,研究氮、磷、钾肥用量及配比对红花产量的影响,探讨氮、磷、钾不同用量及配比下红花生育期各器官中有效成分分配规律。结果表明：红花花产量随氮肥用量的增加而增加,以T5最高,与T1相比增加59.44%,随磷肥和钾肥用量的增加呈先上升后下降趋势,均以T4最大,与T1相比增加42.87%;红花种子产量随氮、磷、钾肥用量的增加呈先上升后下降的趋势,均以T4最高,与T1相比增加116.52%。氮、磷、钾肥不同用量及配比对红花生育期各器官中黄酮含量影响有相同规律,从苗期到成熟期,叶和茎黄酮含量均表现为先下降后上升的趋势,根中黄...     

青海东部农区引进燕麦品种各器官C、N、P生态化学计量学特征变化研究

以4个引种燕麦(Avena sativa)为典型代表,对其不同生育时期根、茎、叶中的C、N、P含量及其化学计量学特征进行测定,探讨不同生育时期燕麦C、N、P元素含量及其化学计量比的变化规律,为燕麦饲草的科学收获提供理论依据。结果表明：燕麦全株C、N、P含量分别为322.30～333.97、17.42～75.62、2.74～5.42 mg·g^-1,燕麦根C、N、P含量分别为298.42～317.92、11.47～73.71、2.82～3.42 mg·g^-1,燕麦茎C、N、P含量分别为311.25～338.86、10.15～75.16、2.44～5.06 mg·g^-1,燕麦叶C、N、P含量分别为330.80～372.47、30.64～113.80、2.59～8.65 mg·g^-1;各器官间C、N、P含量表现为叶>茎>根。此外,燕麦各器官C、N、P含量的积累过程具有一定季节特征,C含量积累过程受生育时期影响较小,表现出较强的稳定性;而N和P含量的积累过程受生育时期影响较大,其在拔节期～抽穗期均...     

国内外致泻大肠埃希氏菌O157:H7的检测标准比较及建议

致泻大肠埃希氏菌O157:H7（STEC O157:H7）是大肠埃希氏菌中致病性最严重的一种食源性致病菌,主要存在于牛肉、牛奶、水果及其制品中,对身体健康造成很大危害,甚至引发死亡。食品中STEC O157:H7检测尤为重要。本文对国内外STEC O157:H7的检测标准进行比较,提出我国标准在样品前处理、快速筛选方法的应用等方面需要加强,以便为该菌快速准确检测提供帮助,实现与国际标准化体系建设接轨,满足实验室检测需要。     

基于全基因组填充重测序关联分析鉴别影响苏山猪初生体尺与乳头数性状的遗传位点

旨在鉴别影响苏山猪初生体尺和乳头数性状的遗传位点,开发可用于辅助育种的分子标记,为苏山猪生长和繁殖性能的持续选育提供理论基础。本试验对269头苏山猪初生仔猪(出生后24 h内)的体尺和乳头数表型进行测定,并采集耳组织样品。基于全基因组重测序及基因型填充策略,利用全基因组关联分析(genome-wide association study, GWAS)和群体分化指数(fixation index,Fst)的方法挖掘与目的性状强关联位点,并对位置功能候选基因开展GO和KEGG分析,确定最有可能的主效基因。本研究共鉴别到4个候选位点,分布在13、14和X染色体上,其中与初生体尺性状显著关联的位点有2个,与乳头数性状显著关联的位点有2个。本研究筛选出1个与体长性状相关的候选基因ACTA2;1个与胸围性状相关的候选基因COL4A6;3个与乳头数性状相关的候选基因ACTA2、CRTAP和SEPTIN6,为苏山猪初生体尺性状和乳头数性状的遗传改良提供了重要的分子标记。     

四十种瓜菜中超氧化物歧化酶含量测定

采用化学发光法测定了40种蔬菜、瓜、果中的超氧化物歧化酶(SOD)的含量,并在此基础上探讨了9种蔬菜中维生素E、C、蛋白对SOD的影响以及它们之间的关系.还观察了蔬菜中SOD的热稳定性.结果表明,不同蔬菜、瓜、果间SOD含量的差异很大,其范围在1.26～176.10×10~(-3)g/g蛋白之间;去除脂溶性维生素E时SOD有不同程度的下降;维生素C及蛋白与SOD无明显对应关系;加热处理后样品的SOD仍得到较好保存.     

Digital Beamforming for Unified Spread Spectrum TT&C System

In TT&C system,unified spread spectrum framework and adaptive antenna digital beamforming technologies are used to track, telemetry and command objects. Constant modulus array (CMA) and adaptive signal canceller estimate the direction of arrival (DOA) of every signal and use the optimum weighted vector to form the beams. The phase weighted vectors estimated from DOA improve the processing gain as well as being utilized in the transmitting of tele command signal to form the beam of specified object. Its processing performance is researched and simulated in this paper.     

Portable ECG Monitor Front- ended Design with C8051 F020

The electrocardiogram (ECG) signal is a recording of the electrical activity on the body surface generated by the heart frequency of which is weak and low. Many researchers devote themselves to get more perfect ECG in many ways. This article introduces the characterize of an ECG signal , then discusses the requirements for the sampling circuit of an ECG. Since there are some shortcoming in common design means, the authors give an effective circuit. The system is composed of a low-cost , low-power and high-performance MCU C8051F020 , amplifier AD620 , right-leg drive circuit ,communication circuit and power management circuit. Then software design is given which is used to sampling and filtering ECG signal. Levkov filter arithmetic and smooth filter arithmetics are used in this design. In the end , the authors advance a distributing and networking ECG monitor system.     

不同生态环境马铃薯维生素C含量分析

对马铃薯参试品种在西北地区不同生态条件下的维生素C含量变化进行方差分析和稳定性分析。结果表明：试点间、品种间以及试点和品种的互作间都存在着极显著差异，6个品种平均维生素C含量为15．04mg／100g，陇薯3号最高，为18．68mg／100g，90-2-10最低。为12．23mg／100g；7个地点平均维生素C含量为15．04mg／100g，青海西宁最高，为16．75mg／100g，青海海南最低，为12．93mg／100g。中薯36这个品种的回归系数〈1，是稳定性较好的品种。