非亲和性稻瘟病菌对水稻的诱导抗性

为研究稻瘟病菌Magnaporthe oryzae不同菌株间的相互作用,选择与单抗性基因系水稻IRBL5-M （携带抗性基因Pi5）表现为亲和性的菌株HN52与非亲和性的菌株HN119为研究对象,将其单独或混合接种到单抗性基因系水稻IRBL5-M中,并通过荧光显微镜观察接种后水稻叶鞘的发病情况及病斑面积,测定接种后水稻内相关抗性基因OsWRKY45、OsNPR1、OsPR10、OsMAPK2的表达量以及活性氧的变化。结果显示,相较于单独接种亲和性菌株,混合接种后单抗性基因系水稻IRBL5-M病斑发病面积减少;混合接种中亲和性菌株HN52菌丝侵染能力降低,侵染菌丝细胞间扩展率显著降低73.13%;同时单抗性基因系水稻IRBL5-M中OsWRKY45、OsNPR1、OsPR10和OsMAPK2抗性基因表达量显著增加,水稻叶片中活性氧含量增加,表明在菌株混合侵染过程中,非亲和性菌株可通过激发水稻的抗性反应来降低亲和性菌株对水稻的侵染程度。     

Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen

The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.     

裸子植物体细胞胚胎发生和人工种子的研究

该文介绍了一种作为森林种子的大规模增值的有效方法－－裸子植物体细胞胚胎发生和人工种子的研制。其内容包括：（１）胚性愈伤组织的诱导与保存；（２）影响体胚发生的因素；（３）体胚的发育与成熟；（４）大规模培养体胚；（５）成熟体胚的干化、包理与人工种子生产；（６）体胚的萌发和转换。最后对人工种子的应用前景进行了讨论。     

Expert System for Artificial Intelligence Blast Furnace Smelting Process

In order to upgrade traditional industry and realize the automation of furnace condition judgement and standardization of operation, an expert system for various furnace conditions and breakdown judgement and operation guidance on blast furnace are developed based on the theory of artificial intelligence and knowledge engineering. The system is fulfilled through C++Builder and combined with the actual production picture. The system has efficient and convenient man-machine interface by using menu operation method. The testing and operation of actual production data in simulation system indicate that the system is exact and reliable and can provide both the warning of various furnace breakdown and corresponding operation guidance.     

An Expert System Approach to the Computer Aided Design of Intelligent Controllers

The idea for an expert system approach to the computer aided design of intelligent controllers (ESCAD1C) is discussed and the idea of two step modelling of an intelligent controller is presented on the basis of emulating human control behaviours to solve complex problems. The structure, functions and characteristics of ESCADTC system , and the design procedures of intelligent controllers are gived. The knowledge base and simulating functions for designing intelligent controllers are also described.     

人工合成甘蓝型油菜高频率再生研究初报

资源材料保存在作物遗传资源的研究与利用中起到非常重要的作用。该实验以不同来源的人工合成甘蓝型油菜KH106，KH109，KH111D为材料，以花序轴，子房，果柄为外植体研究其再生情况，结果表明，由芥蓝和小白菜人工合成的甘蓝型油菜KH106的再生频率极显著高于由羽衣甘蓝和白菜型油菜人工合成的甘蓝型油菜KH109和KH111；与花序轴和子房为外植体再生频率相比，以果柄为外植体平均再生频率最高，差异达到极显著水平；利用不同预处理培养基处理不同时间，结果显示不经过预处理直接接种到分化培养基上再生频率最高。     

The Method of Diagnosing Brain-dysfunction Based on Membership Function and BP Neural Network

Membership functions.formed with the characteristic values obtained by applying the continuous reaction time (CRT) theory from controls and heptic encephalopthy and proposedin this paper.whith these membership functions,the CRT data to be diagnosed are calculated in advance,and then discriminated by BP meural network to differentiate patients with or without braindysfunction.The troubles of low accuracy and efficiency,encountered in training BP network withfuzzy samples.scattered data and extended samples,are solved.     

三江平原井灌水稻区地下水人工补给方法初探

针对三江平原井灌区地下水位逐渐下降、急需恢复的现状,结合国内外地下水人工补给实践,对三江平原井灌区地下水人工补给方法进行了探讨,提出了今后地下水人工补给实践的初步设想,为该区域地下水资源的可持续利用提供了参考依据.     

新兴绿色技术——水生植物修复技术

水体富营养化治理已成为当今世界性难题,解决水体富营养化问题的关键是调控水生生态系统结构,使其恢复自然、健康和稳定的水生生态系统功能,提高水生生态系统的生物净化能力.水生植物是水生生态系统中最重要的初级生产者,在水生生态系统物质和能量循环中处于十分重要的地位.水生植物修复技术具有投资、维护和运行费用低,管理简便,处理效果好,可改善和恢复生态环境,回收资源和能源以及收获经济植物等优点,浮岛技术、人工湿地等技术也取得了显著的成就.植物修复技术作为一种绿色技术,将会有更加广阔的发展天地.