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901.
McDonnell JJ Knowles KE deLahunta A Bell JS Lowrie CT Todhunter RJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(4):530-537
Five young Shiloh Shepherd Dogs (4 males and 1 female) related by a common sire were studied because of progressive pelvic limb weakness and incoordination. All dogs had a spastic paraparesis and pelvic limb ataxia consistent with an upper motor neuron and general proprioceptive lesion between spinal cord segments T3 and L3. Proliferative lesions involving one or more of the articular processes from the 11th thoracic vertebrae to the 2nd lumbar vertebra were observed on radiographs of the thoracolumbar vertebrae. Dorsal compression of the spinal cord was identified during imaging studies at these sites. Abnormalities of the synovial joints and bony proliferation of the involved articular processes were identified at postmortem examination in 2 dogs. The articular processes and associated vertebral arches protruded into the vertebral canal, indenting the dorsal surface of the spinalcord. Degenerative joint disease (DJD) was identified histologically. A compressive myelopathy was diagnosed in the spinal cord. These dogs were affected by a compressive myelopathy as a consequence of vertebral process DJD that likely has a geneticcomponent. The DJD could have been caused by a primary vertebral malformation or an injury to the processes at a young age causing malarticulation. 相似文献
902.
903.
AIM: To investigate the change of Cpn60 content, the alterations of pancreatic enzymes and lysosome, in order to better understand the mechanism of intrapancreatic enzyme activation in acute pancreatitis(AP). METHODS: The AP model was replicated by retrograde infusion of 4% sodium-deoxycholate in the choledocus of SD rats. The levels of amylase in plasma and TNF-α in pancreatic tissue were measured by biochemical technique at 5 h and 10 h after AP induction. The content of Cpn60 and pancreatic enzymes in different compartments of the acinar cells were tested by quantitative protein A-gold immunocytochemistry technique. The change of lysosome in the acinar cells was observed under the electronic microscope. RESULTS: After AP was induced, the levels of amylase in the plasma and TNF-α in the pancreatic tissue increased significantly. Lysosomes with different forms were found inside the acinar cells, and some of them located in the Golgi apparatus. Cpn60 content decreased, which was accompanied by an increase of lipase or chymotrypsinogen content in the pancreatic secretory pathway. CONCLUSION:In the pancreatic acinarcells of AP rats,Cpn60 content decreased,suggesting an insufficient chaperone capacity,and combining with the change of lysosome both in its amount and locat ion,which may take part in the intrapancreatic enzyme activation and the development of AP. 相似文献
904.
WU Xin-zhong HUANG Xian-zhang KE Pei- feng ZHENG Song-bo MA Yan ZHUANG Jun-hua LIN Lin ZOU Guo -lin 《园艺学报》2004,20(10):1916-1918
AIM: To observe free radicals (MDA, NO) and iNOS of patients with severe acute respiratory syndrome (SARS) and to explore its significance. METHODS: MDA, NO2-/NO3- and iNOS were determined in SARS patients during the early, recovery and follow-up stage, front doctors and nurses (contact group) and health people (health control). RESULTS: The level of MDA during first stage was higher than that of recovery stage and the MDA level of recovery stage was higher than that of follow-up stage, contact group, and health control group (P<0.01). The content of NO2-/NO3- during early stage was higher than that of other groups, and the NO2-/NO3- contents of recovery stage, follow-up stage were higher than that of contact group and health control group (P<0.01), respectively. The mean of iNOS during early stage was highest than that of other stages (P<0.01) and the mean of recovery stage was higher than that of contact group (P<0.05), there were no difference in iNOS activity among any other groups (P>0.05). CONCLUSION: The pathological injury in pathogenesis of SARS is related to free radicals. 相似文献
905.
906.
The case details and outcome after surgical decompression of 46 dogs with thoracolumbar intervertebral disc disease with loss of deep pain perception prior to surgery were reviewed. Nineteen dogs (41.3%) recovered with a median follow-up period of 12.5 months. Recovery was defined as an ambulatory paraparesis, or better, with urinary and fecal continence. There was a better outcome in dogs with loss of deep pain for less than 24 hours prior to surgery (19/41; 46.3% recovered) than in dogs without deep pain perception for more than 24 hours (0/5; 0% recovered). Dogs with deep pain perception present at two weeks postoperatively had significantly higher success rate (8/12; 66.7% recovered) than dogs without deep pain perception at this time period (1/10; 10.0% recovered). The return of deep pain perception by two weeks postoperatively can be a useful positive prognostic indicator. 相似文献
907.
引种扁桃抗寒力及冻害成因分析 总被引:1,自引:0,他引:1
通过对两个引种扁桃扶兹(Fritz)和那普瑞尔(Nonpareil)1年生枝条的半致死温度(LT50)拟合、越冬调查、休眠期的含水率及其解刨结构的分析,对扁桃的抗寒力进行了研究,结果表明:Fritz、Nonpareil及CK 1年生休眠枝的半致死温度(LT50)分别为-24.88℃、-20.56℃和-17.15℃;冻害指数分别为10%、45%和87%,抗冻害评级依次为高抗、中抗、易抽。因此,两供试品种都有较强的抗寒能力,而且均强于CK,Fritz的抗寒能力最强。造成1年生枝条冻害的主要原因是生长与休眠临界期枝条的大量失水;其形态解剖学原因是表皮薄,皮层薄壁细胞排列疏松和韧皮薄壁细胞不发达。 相似文献
908.
AIM: To study the effect of acute renal failure (ARF) on the differentiated frequency of Sca-1+ cells from murine fetal liver in irradiated mice. METHODS: The Sca-1+ cells from murine fetal liver were isolated with magnetic cell sorting (MACS) technique, the sex of which was identified by PCR. The 2×104 Sca-1+ cells were transplanted into a lethally irradiated ([60Co], 8 Gy) inbred female mouse. After 8 weeks, these recipient mice were divided to A, B, and C groups at random (A group: irradiated; B group: ARF; C group: ARF and Sca-1+). The mice in B and C groups were induced to ARF with 50% (V/V) glycerin (11.6 mL/kg). 72 hours later, the mice in C group were injected with the fresh prepared Sca-1+ cells again. 8 weeks later, mice were sacrificed, and their kidneys were taken out, fixed and slices were prepared. Fluorescence in situ hybridization (FISH) of renal slices was performed and the pictures of them were taken and analyzed. RESULTS: The cells containing Y chromosome were found in renal slices from the mice in A, B and C groups, which located in epithelial cells of renal tubules, interstitium, glomeruli, and glomerular margin and increased gradually. The double and encircle zone of Y chromosome cells were found in the slices from the mice in B and C groups separately, which was consist of new renal tubules. The differentiation frequency of Sca-1+ cells in kidney in A, B and C groups were (1.65±0.18)%, (8.58±1.34)% and (18.13±1.91)%, respectively, which showed significant difference between former group and later group (P<0.01). CONCLUSION: The differentiation of Sca-1+ cells from murine fetal liver into renal cells and tissue is promoted by physiological microenvironment of acute damage and regeneration. 相似文献
909.
910.