农抗120对水稻纹枯病菌抗生活性试验

采用平板培养法测定农抗120对水稻纹桔菌的抗生试验结果,表明农抗120对水稻纹枯病菌的抗生作用为抑制菌丝生长,使菌丝畸变。菌丝顶端产生不正常的分枝,分枝与主轴成直角,菌丝先端扭曲变形,不形成菌核。农抗120对纹枯菌的抑制生长最低浓度为12.5μg/ml,抑制纹枯菌核形成的最低浓度为100μg/ml,可推迟形成菌核,有效地控制水稻纹枯病流行和扩展。     

配方施用BR-120对棉花经济性状和抗病性的影响

用BR－120（油菜内酯素）或BR－120与尿素、多菌灵配合对棉花进行根外（叶面）喷施试验，结果大多数处理的皮棉产量高于ck（不施用任何肥料和药剂）特别是用BR－120与尿素配合施用时有明显的增产作用，其增产的主要原因是棉花的成铃率提高。施用BR－120等混配剂得当时对棉花的纤维品质也有一定的改良作用，如有的处理纤维增长，比强度提高，但对棉花枯黄萎病没有表明出防治效果。     

Effect of autophagy on L-type calcium channel current induced by gp120V3 in hippocampus neurons

AIM: To observe the influence of autophagy on L-type calcium channel current induced by gp120V3 loop in hippocampal neurons.METHODS: Hippocampal neurons were exteriorized from the newborn rats within 1 d and primarily cultured for 7 d. The neurons were divided into 2 parallel groups, namely control group, gp120V3 group, autophagy inhibitor 3-MA group, gp120V3 + 3-MA group; control group, gp120V3 group, autophagy activator rapamycin group, gp120V3 + rapamycin group. Whole-cell pathch-clamp was used to record L-type calcium channel current. RESULTS: Compared with control group, the density of L-type calcium channel current increased in gp120V3 group and 3-MA group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + 3-MA group(P<0.05). Compared with control group, the density of L-type calcium channel current decreased in rapamycin group(P<0.05). Compared with gp120V3 group, the density of L-type calcium channel current increased in gp120V3 + rapamycin group(P<0.05).CONCLUSION: Autophagy may be involved in the gp120V3 loop-mediated L-type calcium channels in hippocampus neurons.     

农抗120药泥封闭法防治苹果腐烂病试验

研究了农抗120药泥封闭法防治苹果树腐烂病的效果.结果表明:农抗120药泥封闭疗法防治效果优于一般黄泥封闭疗法和40%福美砷可湿性粉剂50倍液、4%农抗120水剂50倍液刮涂疗法.同时,农抗120药泥封闭疗法有促进伤口愈合组织形成的良好作用.     

恢复系N120系列组合高产制种技术

介绍了江西农业大学选育的强恢复系N120的生育期和开花习性及其系列组合秋季高产制种技术.     

鸡传染性支气管炎病毒H120株细胞适应毒的培育研究

以IBH120鸡胚毒适应成纤维细胞（CEF）单层,培养出IBH120细胞弱毒株。电镜观察,可见典型的冠状病毒粒子。IBH120细胞弱毒株经细菌、霉菌、支原体、鸡白血病病毒检测均为阴性,且具有IB病毒特异性。CEF单层接毒后48h左右病毒增殖达到高峰。随着细胞传代次数的增加,C4～C10代H120株细胞毒的毒价（TCID50）逐步增高而趋于稳定,C4～C120代细胞毒的TCID50稳定在107^7.35-8.0mL之间,而C5～C10代IBH120细胞毒的EID50呈下降趋势,变动范围在10^7.3～10^6.5mL。以10^3.5 TCID50／只和10^4.5 TCID50／只的剂量,C4,C8,C10代毒分别滴鼻接种3日龄敏感鸡,免疫鸡的攻毒保护率为80％～100％,病毒分离回收率分别为20％和0。     

农抗120防治小麦白粉病的应用技术研究

1989—1990年在河南省8个县市对农抗120防治小麦白粉病进行了田间试验。结果表明:在4月上中旬,小麦白粉病发生的病株率达15～20％,病叶率10％左右时,应用100ppm农抗120喷雾防治2次,防效达70～85％。防病效果与防治时间、喷药次数和发病程度关系密切。施药越晚,效果越差。农抗120的防治效果优于多菌灵等常用化学药剂,在发病初期喷药2次,防治效果与施用粉锈宁相近,可作为粉锈宁紧缺情况下的首选替补药物。但在病害大流行时施用,作用缓慢,效果较差。     

农抗120防治花生叶斑病药效试验

在山东省经多点试验,用200倍农抗120防治花生叶斑病,防效达69.6～78.1％,比500倍多菌灵防效57.4～66.5％,高11.6～12.2％。并对花生有刺激生长的作用,喷施农抗120的主茎高26.2厘米,侧枝长31.4厘米,单株果重18.8克,比对照主茎高1.3厘米,侧枝长2.3厘米,单株果重增加2.7克,增产11.6～18.5％。     

Artifactual changes in equine blood following storage,detected using the Advia 120 hematology analyzer

Background — Delayed analysis of blood samples may be caused by restricted access to laboratories. Artifactual changes may occur in the measured analytes as a consequence of delayed analysis and may complicate interpretation of the data.
Objective — The purpose of this study was to characterize artifactual changes in equine blood, due to storage, using the Advia 120 hematology analyzer.
Methods — Samples of blood from 5 horses were analyzed using the Advia 120 soon after collection and again after 24 and 48 hours of storage at either 4°C or ambient laboratory temperature (∼24°C).
Results — Delayed analysis of equine blood samples resulted in increased numbers of normocytic hypochromic RBCs, increased numbers of macrocytic hypochromic RBCs, misclassification of granulocytes as mononuclear cells using the basophil reagent method, and pseudothrombocytosis, due to misclassification of ghost RBCs as platelets. The latter artifact was corrected by an amended version of the software. Many of the artifactual changes were identified by morphology flags.
Conclusion — Characteristic changes in cytograms produced by the Advia 120 allowed recognition of artifactual changes in stored equine blood samples. These changes were less pronounced in samples stored at 24°C than at 4°C.