长江源区土地沙漠化防治对策与措施

长江源区的土地沙漠化是最严重的生态环境问题。沙漠化土地主要分布于曲麻莱和治多两县境内的通天河阶地及楚玛尔河滩地 ,在玉树、称多县和塘古拉山乡也有分布。在青藏公路的五道粱至二道沟以东的河滩地区 ,有大片流动沙丘分布。在沱沱河口以东有连片的流动沙丘分布 ,其余地区为不连片的零星流动沙丘和沙漠化土地分布 ,长江源区共有沙漠化土地面积 1 9475 .32k 。土地沙漠化的迅速扩展 ,不仅影响本区生态环境 ,同时直接影响着整个流域的社会经济持续发展。根据本区高海拔寒冷的严酷自然条件 ,依科技为先导 ,建立科技示范工程 ,以保护生态环境为主 ,恢复植被与防治沙漠化土地相结合 ,即采取封育保护或建立生态保护区 ,在条件适宜的地区结合补播、治沙造林 ,运用生物和工程相结合的综合防治土地沙漠化的技术措施 ,建立新的生态系统 ,改善生态环境 ,恢复和重建长江源区的生态环境     

诊断桑树黄化型萎缩病的新方法

本文探讨利用瑞氏染色法和旦尼氏染色法诊断桑树黄化型萎缩病的可能性。试验结果表明,用此方法,在病株枝条或主根的横切面上,韧皮部的病原寄生部位被染成兰色或青色,呈阳性反应,健株组织切片的韧皮部则不着色,呈阴性反应。可作为桑黄化型萎缩病病株的实验诊断手段。     

Detection of genomic DNA of the crayfish plague fungus Aphanomyces astaci (Oomycete) in clinical samples by PCR

A diagnostic procedure, based on a polymerase chain reaction method (PCR) was developed to detect infection of crayfish with the Oomycete Aphanomyces astaci. A set of oligonucleotide primers was designed to specifically amplify A. astaci DNA in the ITS region surrounding the 5.8S rDNA gene. The PCR amplifies a 115 bp amplicon. The specificity of the primers was demonstrated by testing on 27 A. astaci strains and against 20 non-A. astaci Oomycetes and 5 fungal species. Most of the non-A. astaci Oomycete or fungal species included in the study are either known parasites of freshwater crayfish cuticle or can be found in their natural environment. Specificity was also tested against crayfish tissue and some known parasites and bacteria infecting crayfish.

A protocol for the extraction of A. astaci DNA from infected crayfish tissue was developed. The optimised method allows the detection of two genome equivalents of purified A. astaci genomic DNA.

The method was tested on noble crayfish (Astacus astacus), artificially infected with A. astaci. Detection of A. astaci was possible at the very first time of sampling, which was 2 days after the beginning of spore exposure.     

三合激素诱导黄牛发情效果

2003年～2005年应用三合激素对格尔木周边60头产后乏情黄牛进行诱导发情试验,配种60 d后经直肠检查结果表明,注射两次,发情及配种效果明显.     

黄河源区“黑土滩”退化草地CO2排放研究

采用静态箱-气相色谱法,对黄河源区“黑土滩”退化草地CO2释放特征的研究结果表明：“黑土滩”3种不同处理：“黑土滩”植被-土壤系统呼吸（BC）,土壤呼吸（BJ）和土壤微生物呼吸（BL）的CO2释放速率日变化具有相似性,均呈现出明显的单峰型特点,且白天大于夜间;CO2释放速率季节动态变化明显,且3者的变化趋势基本一致;“黑土滩”植被-土壤系统呼吸与地表温度及5cm地温呈极显著的正相关性（P〈0．01）;而土壤微生物呼吸与0～10cm土壤水分含量相关显著（P〈0．05）。     

绵羊胚胎移植技术在柴达木地区的应用

2006年在青海省海西州德令哈市洋嘉生态农业开发有限公司进行了绵羊胚胎移植试验,试验中同期发情和超数排卵处理青海高原毛肉兼用半细毛羊共86只,同期发情率达88.37%,其中将超排效果好的11只羊做为胚胎移植受体羊,同时处理供体羊特克赛尔羊7只。试验结果:11只受体羊平均超数排卵点2.8个/只,7只供体羊平均超数排卵11.9枚,取卵9.7枚,卵子受精率17.65%,移植12枚胚胎,其中1只受体羊移植双胚,最后观察到移植的11只受体羊中妊娠5只,产羔5只(移双胚的羊产1只羊羔),移植受胎率达45.45%,羔羊成活率达100%。     

青海省龙羊峡库区草地资源遥感调查研究

利用 1987年和 2 0 0 0年美国陆地卫星TM数据资料 ,对青海省龙羊峡库区 13年来草地的面积、分布、发展和演变情况进行了调查 ,发现龙羊峡库区天然草地面积 13年中减少了6 984 .0 9hm2 ,平均年递减速率为 0 .14 % ,草地盖度、高度、产量等方面均有不同程度的下降 ,草地盖度平均下降 5 %～ 10 % ,牧草高度下降 3～ 2 8cm ,牧草产量均呈现下降趋势     

贵州喀斯特地区牧草引种试验评价

对国内外引进的6个禾本科牧草品种,9个豆科牧草品种以及喀斯特地区的4个对照品种,在贵州独山地区进行引种试验。结果表明：各参试品种生长良好,能安全越冬、越夏,一年可刈割5～6次,鲜草产量达36558．2～66450．0kg／hm2,干草产量10771．0～19869．0kg／hm2。综合性状表现较好的依次为鸭茅Totter,Wana,Tuoa;多年生黑麦草Nui,Bronsys;白三叶Alice,C6151,Prop,Pitua,Sustain;百脉根Mareb,Lel。     

Freshwater snail distribution related to environmental factors in Banco National Park,an urban reserve in the Ivory Coast (West Africa)

Freshwater snails and environmental variables were studied at seven stations along the Banco River (Ivory Coast;West Africa) from March to October 2008. Two species (Physa marmorata (Physidae) and Bulinus forskalii (Planorbidae) were recorded only at one sampling site. This station which received domestic sewage from the neighbouring cities was characterized by the highest conductivity and pH and the lowest values of dissolved oxygen. Multiple stepwise regression analysis showed that snail densities were probably influenced by conductivity, dissolved oxygen, pH, and canopy cover. Also, snail dynamics showed a seasonal pattern, with peak population densities and recruitment of young generations during rainy periods. This study highlighted the impacts of sewage on snail diversity and distribution in the Banco River. Overall, this paper strengthens the viewpoint that the Banco River management should be implemented to lessen the impact of anthropogenic activities on the ecology of the Banco National Park.