不同沟垄覆盖下土壤水热效应对旱作马铃薯生长及产量的影响

针对宁南山区春季降雨少、低温不利于马铃薯出苗,而作物生育中后期高温胁迫限制马铃薯块茎形成导致减产等问题,于2016年设置垄覆地膜沟内覆盖塑料地膜(DM)、玉米秸秆(JG)、生物降解膜(SM)、麻纤维地膜(MM)、液态地膜(YM)及沟不覆盖(BM),以传统平作为对照(CK),研究沟垄覆盖模式对土壤温度、土壤水分、旱作马铃薯生长及块茎产量的影响.结果表明:处理DM,SM,MM和YM马铃薯生育期耕层土壤温度表现出增温效应,而处理JG和BM表现为降温效应,其中处理DM增温效果和处理JG降温效果最佳.处理YM,JG对马铃薯生长前期保水效果较好,分别较CK显著提高13.3%,27.0%,而处理JG,DM对生育中后期的保墒效果较佳,分别较CK显著提高22.4%,13.2%.处理JG可显著促进马铃薯生长,其增产效果最为显著,DM次之,分别较CK增产47.8%,44.8%,其他处理较CK差异不具有统计学意义.可见,在宁南山区实施垄覆地膜沟覆秸秆模式可调控土壤水热环境,对旱作马铃薯生长及增产效果最佳.     

Greater humification of belowground than aboveground biomass carbon into particulate soil organic matter in no-till corn and soybean crops

Quantifying the amount of carbon (C) incorporated from decomposing residues into soil organic carbon (C_S) requires knowing the rate of C stabilization (humification rate) into different soil organic matter pools. However, the differential humification rates of C derived from belowground and aboveground biomass into C_S pools has been poorly quantified. We estimated the contribution of aboveground and belowground biomass to the formation of C_S in four agricultural treatments by measuring changes in δ¹³C natural abundance in particulate organic matter (C_POM) associated with manipulations of C₃ and C₄ biomass. The treatments were (1) continuous corn cropping (C₄ plant), (2) continuous soybean cropping (C₃), and two stubble exchange treatments (3 and 4) where the aboveground biomass left after the grain harvest was exchanged between corn and soybean plots, allowing the separation of aboveground and belowground C inputs to C_S based on the different δ¹³C signatures. After two growing seasons, C_POM was primarily derived from belowground C inputs, even though they represented only ∼10% of the total plant C inputs as residues. Belowground biomass contributed from 60% to almost 80% of the total new C present in the C_POM in the top 10 cm of soil. The humification rate of belowground C inputs into C_POM was 24% and 10%, while that of aboveground C inputs was only 0.5% and 1.0% for soybean and corn, respectively. Our results indicate that roots can play a disproportionately important role in the C_POM budget in soils. Keywords Particulate organic matter; root carbon inputs; carbon isotopes; humification rate; corn; soybean.     

Herbicide options for effective weed management in dry direct-seeded rice under scented rice-wheat rotation of western Indo-Gangetic Plains

Farmers' participatory field trials were conducted at Madhuban, and Taraori, the two participatory experimental sites/locations of the Cereal Systems Initiative for South Asia (CSISA), a collaborative project of IRRI and CIMMYT in Karnal district of Haryana, India, during Kharif (wet season) 2010 and 2011. This research aimed to evaluate preemergence (PRE) and postemergence (POST) herbicides for providing feasible and economically viable weed management options to farmers for predominant scented rice varieties. Treatments with pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST had lower weed biomass at 45 days after sowing (DAS). At Madhuban, highest grain yield of scented basmati rice (3.43 t ha⁻¹) was recorded with the sequential application of pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST. However, at Taraori, yields were similar with pendimethalin or oxadiargyl PRE fb bispyribac-sodium and/or azimsulfuron POST. Applying oxadiargyl by mixing with sand onto flooded field was less effective than spray applications in non-flooded field. The benefit-cost ratio of rice crop was higher with herbicide treatments at both sites as compared with the non-treated weed-free check except single PRE and POST applications and sequential application of oxadiargyl PRE fb oxadiargyl PRE. In a separate experiment conducted at Nagla and Taraori sites, scented rice cultivars' ('CSR 30′ and 'Pusa 1121′) tolerance to three rates of azimsulfuron (15, 25, and 35 g ai ha⁻¹) was evaluated over two years (2010 and 2011). CSR 30 (superfine, scented) was more sensitive to higher rates (35 g ai ha⁻¹) of azimsulfuron as compared to Pusa 1121 (fine, scented). Crop injuries were 8 and 28% in case of CSR 30; 5 and 15% in Pusa 1121 when applied with azimsulfuron 25 and 35 g ai ha⁻¹, respectively. Azimsulfuron applied at 35 g ai ha⁻¹ reduced yield in both cultivars but in CSR 30 yield reduction was twofold (11.5%) as that of Pusa 1121 (5.2%).     

仿生法评定抗草甘膦玉米和转Bt基因玉米的酶水解物能值的研究

本试验旨在分析抗草甘膦玉米和转Bt基因玉米原料及饲粮与同源非转基因玉米原料及饲粮体外总能消化率以及酶水解物能值,为转基因玉米的营养实质等同性仿生评定方法的研究提供参考。试验采用单因素完全随机设计,使用单胃动物仿生消化系统模拟饲料原料和饲粮在鸡胃肠道的消化过程,分析同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应的3种玉米-豆粕饲粮在不同体外模拟消化阶段的干物质消化率、总能消化率和酶水解物能值的差异。结果表明:同源非转基因玉米、抗草甘膦玉米和转Bt基因玉米以及对应饲粮在常规概率成分含量上是相似的。抗草甘膦玉米及饲粮与同源非转基因玉米及饲粮相比,在干物质和能量胃消化率、全消化道消化率及酶水解物能值上均没有显著差异(P0.05)。转Bt基因玉米全消化道总能消化率低于同源非转基因玉米(P=0.03,变异系数=0.50%),对应玉米饲粮的酶水解物能值则高于同源非转基因玉米饲粮(P=0.02,变异系数=1.12%),但均处于仿生消化系统测试的误差范围内(变异系数≤1.64%)。由此可见,抗草甘膦玉米的酶水解物能值与同源对照玉米没有差异,而转Bt基因玉米存在统计学意义上的差异,但所有的测值均处于仿生消化系统的测试误差之内。仿生法发现的差异是否具有生物学意义有待体内试验验证。仿生法可为转基因饲料营养等同性研究提供一种新方法。     

油茶高接换冠枯桩现象调查与研究

为了探寻影响油茶高接换冠后枯桩的相关因素,为今后油茶高接换冠技术改良提供理论依据,调查研究了嫁接方法、接穗品种、砧木粗度、留截高度和嫁接方位等因素对油茶高接换冠后枯桩现象的影响。结果表明:5种因素对油茶枯桩程度影响极显著。其中,采用插皮腹接、砧木直径2~6 cm枯桩程度最低;采用‘华硕’和‘湘林XLJ14’其枯桩程度显著低于其它品种;留截高度越低枯桩程度越低;嫁接方位在外侧枯桩程度最低。偏相关分析结果表明,留截高度对油茶枯桩程度的影响最大;其次是嫁接方法、砧木直径和嫁接品种;而嫁接方位对油茶枯桩程度的影响最小。     

Vineyard soil bacterial diversity and composition revealed by 16S rRNA genes: Differentiation by geographic features

Here, we examine soil-borne microbial biogeography as a function of the features that define an American Viticultural Area (AVA), a geographically delimited American wine grape-growing region, defined for its distinguishing features of climate, geology, soils, physical features (topography and water), and elevation. In doing so, we lay a foundation upon which to link the terroir of wine back to the soil-borne microbial communities. The objective of this study is to elucidate the hierarchy of drivers of soil bacterial community structure in wine grape vineyards in Napa Valley, California. We measured differences in the soil bacterial and archaeal community composition and diversity by sequencing the fourth variable region of the small subunit ribosomal RNA gene (16S V4 rDNA). Soil bacterial communities were structured with respect to soil properties and AVA, demonstrating the complexity of soil microbial biogeography at the landscape scale and within the single land-use type. Location and edaphic variables that distinguish AVAs were the strongest explanatory factors for soil microbial community structure. Notably, the relationship with TC and TN of the <53 μm and 53–250 μm soil fractions offers support for the role of bacterial community structure rather than individual taxa on fine soil organic matter content. We reason that AVA, climate, and topography each affect soil microbial communities through their suite of impacts on soil properties. The identification of distinctive soil microbial communities associated with a given AVA lends support to the idea that soil microbial communities form a key in linking wine terroir back to the biotic components of the soil environment, suggesting that the relationship between soil microbial communities and wine terroir should be examined further.     

Effects of organic matter addition on phosphorus availability to flooded and nonflooded rice in a P‐deficient tropical soil: a greenhouse study

Addition of organic matter (OM) to flooded soils stimulates reductive dissolution of Fe(III) minerals, thereby mobilizing associated phosphate (P). Hence, OM management has the potential to overcome P deficiency. This study assessed if OM applications increases soil or mineral fertilizer P availability to rice under anaerobic (flooded) condition and if that effect is different relative to that in aerobic (nonflooded) soils. Rice was grown in P‐deficient soil treated with combinations of addition of mineral P (0, 26 mg P/kg), OM (0, ~9 g OM/kg as rice straw + cattle manure) and water treatments (flooded vs nonflooded) in a factorial pot experiment. The OM was either freshly added just before flooding or incubated moist in soil for 6 months prior to flooding; blanket N and K was added in all treatments. Fresh addition of OM promoted reductive dissolution of Fe(III) minerals in flooded soils, whereas no such effect was found when OM had been incubated for 6 months before flooding. Yield and shoot P uptake largely increased with mineral P addition in all soils, whereas OM addition increased yield and P uptake only in flooded soils following fresh OM addition. The combination of mineral P and OM gave the largest yield and P uptake. Addition of OM just prior to soil flooding increased P uptake but was insufficient to overcome P deficiency in the absence of mineral P. Larger applications of OM are unlikely to be more successful in flooded soils due to side effects, such as Fe toxicity.     

中国荷斯坦牛干奶期长度的群体特征及其与生产性能的关系

本研究旨在探究北京地区中国荷斯坦牛干奶期长度（DPL）的变化规律及其变异对奶牛各项生产性能的影响，包括产奶性能、繁殖性能、初乳品质和乳房健康。收集了北京地区34个牧场出生于2009—2018年136 231头中国荷斯坦牛的干奶日期、生产性能记录、繁殖性能记录等。通过整理试验牛群的干奶记录，揭示了北京地区中国荷斯坦牛1胎和2胎DPL的群体规律，利用固定模型分析了胎次、产犊季节、场-产犊年和初产月龄对DPL的影响；此外，利用固定模型分析了DPL的变异对日产奶量、乳脂率、乳蛋白率、首末次配种间隔（interval from first to last insemination，IFL）、产犊至首次配种间隔（interval from calving to first insemination，ICF）和初乳品质的影响，利用Logistic回归过程分析了DPL的变异对首次配种受胎率（conception rate of first insemination，CR）、首次配种56 d不返情率（56-days non-return rate of first insemination，NRR56）、难产率和乳房健康的影响。研究结果显示，北京地区中国荷斯坦牛DPL平均为58.81 d，胎次、产犊季节、场-产犊年和初产月龄均对DPL有显著影响（P<0.05）。DPL的变异对日产奶量、乳蛋白率、ICF、CR、NRR56、难产率和乳房健康有显著影响（P<0.05）。干奶期短的奶牛测定日平均乳蛋白率、CR和NRR56更高；DPL适中的奶牛测定日平均产奶量更高、乳房健康水平更好、ICF更短、难产率更低。本研究在国内利用大规模牛群数据揭示了中国荷斯坦牛DPL的群体规律，可为中国规模化奶牛场提升管理水平提供参考。     

不同方法制备的雷竹竹沥化学成分分析

分别采用烧制法、干馏法和渗漉法3种不同方法提取雷竹(Phyllostachys praecox)竹沥,运用气相色谱-质谱联用技术(GC-MS)分析所含化学成分的差异。结果表明,烧制法提取竹沥检出峰104个,可定性成分为20个,含量占总检出成分的62.89%;干馏法提取竹沥检出峰100个,可定性成分21个,含量占总检出成分的57.14%;渗漉法提取竹沥检出峰99个,可定性成分33个,含量占总检出成分的64.81%。雷竹竹沥所含的有机化合物含量与不同的提取方法有关,有机酸类化合物提取应采用烧制法;醛类化合物、醇类化合物提取宜采用渗漉法;酚类化合物提取采用干馏法最优。     

An Overview of Research on Microbial Herbicide

The development of microbial herbicide for weed control has been serious- ly regarded all over the world and has made rapid progress in recent years. This paper presents a comprehensive review on domestic and abroad research progress of microbial herbicide and mainly introduces the microbial herbicide products and mi- crobial sources with commercialization potential, and then proposes an outlook for their development tendency and application prospect.