控制土壤含水量对蔬菜产量及露地菜田水分渗漏量的影响

【目的】通过揭示不同土壤含水量对蔬菜产量及蔬菜地土壤水分渗漏量的影响,为蔬菜生产中合理灌溉提供指导。【方法】通过3年田间试验,利用TDR连续监测土壤含水量,运用田间定位通量法计算土体水分渗漏量,分析不同水分处理下蔬菜产量和土体水分渗漏量之间的差异。【结果】传统水分处理下蔬菜地水分累积渗漏量为982mm,其中蔬菜生长期内累积渗漏量为748mm,占蔬菜生长期内供水量的36%;优化水分处理和充分水分处理下蔬菜地水分累积渗漏量230mm和468mm,其中蔬菜生长期内水分累积渗漏量分别144mm和293mm,占各处理下供水量的9%和17%。除2002年优化和充分水分处理下花椰菜产量高于传统水分处理下花椰菜产量并达到显著水平外,其余年份蔬菜产量并无显著差异。【结论】保持土壤含水量在蔬菜生长有效土壤含水量50%～80%的优化水分处理在蔬菜生产中有很大的推广价值。     

不同条件下提取牡丹花的成分及分析

采用有机溶剂提取法,石油醚作为溶剂,设置不同的提取温度、时间及料液比提取牡丹干花成分,以有效成分的百分含量为评价标准,通过正交试验发现在26℃、牡丹干花(g):石油醚(mL)=1:4、提取4h这样的条件组合下,提取效果最佳。以气相色谱质谱联用仪对提取成分进行分析,共检出78种化合物,其中烷烃类化合物含量最大(51.30%),其次是醇类化合物(7.14%),酯类化合物(2.34%),酮类化合物(0.14%),醛类化合物(0.13%),酚类化合物最少(0.04%)。对牡丹花成分的研究,可为牡丹花的综合开发提供依据。对这些有差异的提取条件进行研究,可有针对性的选择提取方法及条件,对于获得高品质的产品具有重要作用。     

六种试剂盒提取血液基因组DNA效果比较

为筛选一种高效、快捷的血液基因组DNA提取试剂盒,采集24份新鲜西藏绵羊血液样本,选用6种市售商业化试剂盒（LifeFeng柱式试剂盒DK601和非柱式试剂盒DK602,康为世纪非柱式试剂盒CW0544和柱式试剂盒CW0546,上海生工非柱式试剂盒SK8224和柱式试剂盒SK8254）,比较所提DNA的浓度、纯度、得率以及对嗜吞噬细胞无形体16S rRNA进行巢式PCR扩增,综合评价不同试剂盒DNA提取效果.结果表明,应用LifeFeng柱式试剂盒DK601提取的DNA浓度和纯度均最高,得率较高,分别达到75.63 ng/μL、1.894（OD260/OD280）、18.91 ng/μL;且嗜吞噬细胞无形体16S rRNA基因的PCR扩增条带清晰度最优.因此,LifeFeng DK601可作为提取绵羊血液基因组DNA及嗜吞噬细胞无形体（Anaplasma phagocytophilum）病原鉴定的首选试剂盒.     

Microemulsion-based palm kernel oil extraction using mixed surfactant solutions

This study introduces a novel technique using surfactant microemulsion-based oil seed extraction. To achieve this objective, microemulsion formation with palm kernel oil was studied first. Then, the selected microemulsion system was used for palm kernel extraction. The results showed that the mixed surfactant of 3 wt% Comperlan KD and either 0.1 wt% Alfoterra145-5PO or 145-8PO provided an ultralow interfacial tension with the palm kernel oil (0.0197 and 0.0359 mN/m, respectively). By using those two aqueous surfactant systems for palm kernel oil extraction, the extraction efficiency was 93.99 and 94.13% at the optimum crushed kernel size between 0.212 and 0.425 mm, using 1 g seed load to 10 ml of solution and 30 min of contact time. The extracted oil quality was evaluated for water content, fatty acids composition and surfactant partitioning into oil phase. The results showed that the quality of the oil obtained using the surfactant microemulsion-based technique is of similar or better quality than when extracted by hexane solvent.     

Responses of Lowland,Upland and Aerobic Rice Genotypes to Water Limitation During Different Phases

Rice yield reduction due to water limitation depends on its severity and duration and on the phenological stage of its occurrence. We exposed three contrasting rice genotypes, IR64, UPLRi7 and Apo (adapted to lowland, upland and aerobic conditions, respectively), to three water regimes (puddle, 100% and 60% field capacity) in pots during the vegetative (GSI), flowering (GSII) and grain filling (GSIII) stages. Stress at all the three stages significantly reduced yield especially in lowland genotype IR64. Effect of water limitation was more severe at GSII than at the other two stages. Stress at GSI stage reduced both source activity (leaf area and photosynthetic rate) and sink capacity (tiller number or panicle number per pot). When stress was imposed at GSII, spikelet fertility was most affected in all the three genotypes. In both GSII and GSIII, although leaf area was constant in all the three water regimes, estimated relative whole-plant photosynthesis was strongly associated with yield reduction. Reduced photosynthesis due to stress at any given stage was found to have direct impact on yield. Compared to the other genotypes, Apo had deeper roots and maintained a better water relation, thus, higher carbon gain and spikelet viability, and ultimately, higher biomass and productivity under water-limited conditions. Therefore, screening for these stage-dependent adaptive mechanisms is crucial in breeding for sustained rice production under water limitation.     

极香罗勒芳香油成分GC-MS分析

采用超临界CO2萃取技术,对极香罗勒芳香油进行了萃取,并利用气相色谱—质谱法(GC-MS)对芳香油成分进行了鉴定。结果表明:芳香油的主要成分为萜类物质共34种,占总芳香油的46.47%;脂肪酸类3种,占总芳香油的34.61%;芳香族醚类化合物有3种,总芳香油的7.84%;酯类有2种,占总芳香油的3.91%;烃类2种,占总芳香油的1.86%。芳香油中相对含量最高的是亚麻酸,占总芳香油的27.69%;其次是芳樟醇,占总芳香油的12.96%;其它含量较高的成分依次是(+)表-双环倍半水芹烯(7.53%)、m-丁香酚(5.88%)。     

夏季水分管理对梨橙果实发育和产量品质的影响

干旱缺水是导致果园低产、低效的主要原因之一。重庆三峡库区降水丰沛,但水资源在时空上分布很不均匀,许多坡地果园水源紧缺,季节性干旱十分突出。2002年7月至2006年1月,通过用叶片水分亏缺与果实膨大相关性的研究以及水分管理模式,对柑橘生长和发育的影响进行试验研究,总结出了一套适合坡地柑橘园的水分管理模式。     

水分胁迫对紫花苜蓿叶水势、蒸腾速率和气孔导度的影响

采用盆栽水分试验,研究了不同土壤水分条件下紫花苜蓿(Medicago sativa)叶水势、蒸腾速率和气孔导度的变化规律及相互关系,以期揭示其对土壤水分胁迫的气孔响应机制。结果表明:苜蓿叶水势、蒸腾速率(T_r)、气孔导度(G_s)均随水分胁迫加剧而降低,三者日变化均呈双峰曲线特征,日平均值表现为充分供水>轻度胁迫>中度胁迫>重度胁迫。最低叶水势随土壤水分降低而降低;在中度和重度胁迫下,叶水势和蒸腾速率日变化峰值出现的时间提前,当叶水势为-4.68 MPa或T_r为3.27 g·m^-2·h^-1时,气孔开始关闭;在充分供水和轻度胁迫下,T_r越高,叶水势越低,且G_s随着T_r增加而增加。叶水势的变化除受土壤水分影响外,还与叶片生长发育密切相关。     

Integrated management of sensitive catchment systems

Until recently, ‘land use’ was regarded as a single function: in rural areas of the UK this simply meant ‘farming’ or, in the uplands, ‘forestry’. However, there is now growing recognition of the multiple use of land, and farming or forestry must compete with other functions, in particular water supply. Links between hydrological pathways and stream water quality are described as a context for understanding the transport of pollutants to the river system. The concept of landscape sensitivity is then described and applied to the topics of soil erosion and nitrate leaching. Based on these analyses, guidelines for integrated management of sensitive catchment systems are proposed.     

解淀粉芽胞杆菌HAB-6抑菌活性成分的分析

为明确解淀粉芽胞杆菌Bacillus amyloliquefaciens HAB-6菌株的抑菌活性物质,采用平板对峙法检测其对17种植物病原真菌的拮抗能力,通过硫酸铵沉淀法、酸沉淀法、有机溶剂萃取法提取HAB-6菌株的活性物质,并检测其稳定性。结果表明:解淀粉芽胞杆菌HAB-6菌株对17种植物病原真菌均具有良好的抑菌活性,抑制率可达45.48%~72.20%;不同提取方法中仅正丁醇粗提物有抑菌活性,在5 mg/m L浓度下抑菌圈直径达21.49 mm;正丁醇粗提物经紫外线照射120 min后,抑菌活性为对照的78%,而经100℃高温处理后抑菌活性仍为对照的91%,表明HAB-6菌株的活性物质具有较好的热稳定性;但不耐强酸强碱,其适宜的p H为5.0~9.0。正丁醇粗提物可抑制芒果炭疽病菌孢子萌发和菌丝生长,能有效抑制芒果炭疽病菌对芒果果实的侵染。表明HAB-6菌株抑菌谱广,其正丁醇提取物具较强的抑菌活性和较好的稳定性,具有良好的开发应用前景。