水分亏缺对小麦抽穗期穗轴维管束系统的影响

利用盆栽试验研究了水分亏缺对抽穗期小麦穗轴维管束系统的影响。结果表明:穗轴大、小维管束数目、横截面积、导管直径和韧皮部面积沿穗轴由下到上依次减小。但下降幅度因维管束种类、穗轴部位而异。同正常供水处理相比，中度水分亏缺下穗轴大、小维管束数目、面积、导管直径和韧皮部面积有所减小。重度水分亏缺则使穗轴大、小维管束数目、面积、导管直径和韧皮部面积明显减小。表明水分亏缺显著改变了小麦穗轴维管束系统的显微解剖结构。     

谷子Ch型显性雄性核不育花药发育的细胞形态学研究

对谷子Ch型显性雄性核不育花药发育的细胞形态学观察发现,其杂合株花粉发育正常,但开花时花药不开裂;纯合株自造孢细胞期到花粉积累淀粉时均有败育发生,但主要在小孢子期;杂合株的药室壁细胞药隔发育正常;纯合株的药室壁细胞药隔则发生各种异常,可以认为这是其雄性败育的原因.     

STAT3 mediates PDGF-BB-induced Pim-1 expression in rat vascular smooth muscle cells

AIM: To study the expression of Pim-1 in vascular smooth muscle cells (VSMCs) induced by platelet-derived growth factor BB (PDGF-BB). METHODS: VSMCs isolated from rats were treated with different concentrations of PDGF-BB for different time. The proliferation of VSMCs was detected by cell counting. The mRNA expression of Pim-1 was measured by real-time RT-PCR. The STAT3 activity was determined by Western blotting. Actinomycin D, AG490, and small interfering RNA (siRNA) for Pim-1 or STAT3 were used to investigate the underlying mechanisms. RESULTS: Pim-1 gene silencing attenuated the proliferation of VSMCs in response to PDGF-BB. The mRNA expression of Pim-1 was up-regulated by PDGF-BB at concentrations of 10 μg/L~50 μg/L for 1 h, and was maximally induced at the concentration of 20 μg/L. The time of Pim-1 mRNA expression maximally occurred 30 min after PDGF-BB exposure. Incubation of VSMCs with PDGF-BB resulted in a significant activation of STAT3. VSMCs pretreated with actinomycin D showed a significant decrease in the mRNA expression of Pim-1. Treatment with AG490 or knockdown of STAT3 in VSMCs resulted in inactivation of STAT3, and significantly suppressed the mRNA expression of Pim-1. CONCLUSION: PDGF-BB-induced VSMC proliferation is partly attributed to Pim-1. VSMCs strongly increase Pim-1 mRNA upon stimulation with PDGF-BB, and STAT3 signaling pathway appears to be efficient for regulation of Pim-1 expression. This process may play a critical role in development of vascular remodeling.     

犬停循环／再灌注心肌保护合剂研究

应用心肌保护合剂与Ｓｔ．Ｔｈｏｍａｓ停搏液对杂种犬进行了停循环／再灌心肌保护的经性研究，监测了心肌过氧化脂质含量、超氧化物歧哗酶活性，肌酸磷酸激性及心肌含水量等指标。结果表明：我们所筛选的心肌保护合剂可减少自由基的产生，增强清除自由基的能力，降低心肌损伤的程度和推迟     

Unilateral external jugular vein aneurysm in a dog

An 11-month-old Staffordshire Bull Terrier was referred with a two-month history of fluctuating unilateral jugular groove swelling, which appeared to enlarge after exercise. There was no history of trauma. Multimodal imaging findings (using transdermal and transesophageal ultrasound and dual phase computed tomography angiography) were consistent with large, saccular, left jugular vein aneurysm, running parallel to the left carotid artery. There did not appear to be any arteriovenous communication present. There were no cardiac abnormalities found on echocardiography. Following surgical excision, histopathological analysis supported the clinical suspicion of a congenital external jugular venous aneurysm.     

Pre-procedural femoral vessel ultrasound in dogs with patent ductus arteriosus: diameter,image quality and relationship with arterial catheterization

IntroductionObjectives: Patent ductus arteriosus (PDA) in dogs is often treated via minimally invasive transvascular occlusion using femoral artery access. This study compared ultrasound-derived diameter and image quality of the right femoral artery (RFA) and vein (RFV) in dogs with PDA using a linear ultrasound probe (L-P) and phased-array transthoracic echocardiography probe (TTE-P). The case outcome was assessed.Animals, materials & methodsForty-five client-owned dogs with PDA were prospectively enrolled. Ultrasound-measured RFA and RFV diameters were obtained on images acquired with both probes pre-operatively and compared using Bland–Altman plots. The image quality of RFA and RFV was scored on L-P and TTE-P images.ResultsComparison of RFA and RFV diameter from L-P versus TTE-P images revealed: [Mean difference (limits of agreement): RFA = 0.009 mm (−0.78–0.79 mm), RFV = 0.523 mm (−1.75–2.79 mm)]. Image quality scores were significantly higher for L-P than TTE-P (P < 0.0001). In six small dogs, measurable images were unattainable with TTE-P. Dogs of similar body weight had variable RFA diameters. Twenty-seven dogs had RFA catheterization. In 21/27 dogs, RFA diameter exceeded the external diameter of the introducer used for catheterization, and in 6/27, it was smaller.ConclusionsPre-procedural ultrasound of the RFA in dogs with PDA is useful given variable RFA diameter relative to body weight. Despite poorer image quality, RFA diameters from TTE-P images were very similar to L-P images on average, suggesting TTE-Ps are suitable for pre-procedural planning in most dogs. Vasospasm, hypotension or differences in the location of ultrasound measurement versus catheterization might produce variation in pre-procedural versus intraoperative RFA size.     

Outcome clinical audit: analyses of interventional closure of patent ductus arteriosus in dogs

ObjectivesThe objectives of this study were to determine whether conducting a clinical audit was achievable in a group of centres that perform interventional cardiac procedures and to report the success and complications rates in dogs diagnosed with patent ductus arteriosus.MethodsThis was a multicentre, European-wide, prospective study. Patient data were entered into a bespoke database prior to commencing interventional closure of patent ductus arteriosus in all animals undergoing this procedure during the study period. The database was designed to gather clinical audit information, after completion of the procedure, such as discharge outcome, complication rate, and medium-term outcome.ResultsA total of 339 cases were included from five participating centres. The process of performing clinical audit was achieved in all centres. Successful discharge outcome was 95.9% with a complication rate of 4.1%. The procedure-related mortality was 0.6%. 149 cases (43.9%) were either lost to follow-up or had not yet had a follow-up within the time period. Of the remaining 169 cases in which follow-up was available, 157 (92.9%) cases had a successful medium-term outcomeConclusionsThis study demonstrates that the process of performing a clinical audit is achievable in veterinary clinical interventions across different centres. These results provide a benchmark for future comparison in our ongoing clinical audit and validate the process of clinical audit for other centres performing cardiac interventions. The use of clinical audit should be considered in other aspects of veterinary medicine.