全文获取类型
收费全文 | 1418篇 |
免费 | 82篇 |
国内免费 | 127篇 |
专业分类
林业 | 67篇 |
农学 | 124篇 |
基础科学 | 47篇 |
114篇 | |
综合类 | 457篇 |
农作物 | 130篇 |
水产渔业 | 66篇 |
畜牧兽医 | 398篇 |
园艺 | 156篇 |
植物保护 | 68篇 |
出版年
2024年 | 15篇 |
2023年 | 41篇 |
2022年 | 51篇 |
2021年 | 59篇 |
2020年 | 41篇 |
2019年 | 62篇 |
2018年 | 38篇 |
2017年 | 68篇 |
2016年 | 60篇 |
2015年 | 80篇 |
2014年 | 79篇 |
2013年 | 85篇 |
2012年 | 123篇 |
2011年 | 138篇 |
2010年 | 101篇 |
2009年 | 103篇 |
2008年 | 83篇 |
2007年 | 69篇 |
2006年 | 56篇 |
2005年 | 41篇 |
2004年 | 37篇 |
2003年 | 39篇 |
2002年 | 24篇 |
2001年 | 14篇 |
2000年 | 18篇 |
1999年 | 23篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 17篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 2篇 |
1991年 | 10篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
1973年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有1627条查询结果,搜索用时 31 毫秒
111.
Immunohistochemical characterization of canine prostatic carcinoma and correlation with castration status and castration time 总被引:2,自引:0,他引:2
K. U. Sorenmo M. Goldschmidt F. Shofer C. Goldkamp J. Ferracone 《Veterinary and comparative oncology》2003,1(1):48-56
The purpose of this study was to characterize canine prostate cancer using immunohistochemical staining specific for acinar and urothelial/ductal tissue and correlate these results with the dogs' castration status/castration time. Seventy dogs with prostate cancer were included, 71% were castrated and 29% were intact. Compared with an age‐matched control population, castrated dogs were at increased risk of prostate cancer, odds ratio 3.9. Immunohistochemical staining was performed on 58 cases. Forty‐six of the 58 stained positive for cytokeratin 7 (CK 7) (ductal/urothelial origin) and one of the 58 stained positive for prostate‐specific antigen. Dogs with CK 7‐positive tumours were younger when castrated than dogs with CK 7‐negative tumours, 2 versus 7 years (P = 0.03); dogs castrated at ≤2 years of age were more likely to be CK 7‐positive (P = 0.009). These results show that most canine prostatic carcinomas are of ductal/urothelial, androgen‐independent origin. This is consistent with the epidemiological findings, showing increased risk in castrated dogs. Canine prostate cancer may, therefore, not be a realistic model for the human disease. 相似文献
112.
Seedling crops of gazania, geranium, marigold, mimulus, pansy, verbena and vinca were grown to 60% anthesis in three greenhouse temperature regimes: (a) 26 °C day/6 ± 3 °C night (LT), with a 16.5 °C mean daily temperature (MDT), (b) 26 °C day/17 ± 4 °C night, with a 21 °C MDT, and (c) variable day/night temperatures in response to heating and cooling by a phase change material energy storage module (PCM) with an 8 ± 2 °C night and a 16 °C MDT. Crops bloomed from 2 to 17 days earlier in the 26 °C/17 °C greenhouse. The morphological characteristics of all cultivars of gazania, mimulus, pansy and verbena, geranium and vinca were similar regardless of greenhouse temperature regime. Marigold diploid and triploid cultivars and mimulus were morphologically more variable than the other bedding plant species. Crops grown in the PCM and LT greenhouses had virtually identical morphological characteristics and bloomed in about the same number of days. Plant height was not affected by DIF environments in the greenhouses. The PCM and LT greenhouses had a similar MDT, but the distribution of numbers of hours of mean daily temperatures was different. 相似文献
113.
本文介绍了在现代教育思想和教育理论的指导下,结合教学实践,对中等职业学校技工学校机械类焊接专业的专业课<焊工工艺学)CA1课件的设计和制作进行了分析和研究,利用Macromedia公司的多媒体制作软件Authorware7,02设计和制作<焊工工艺学>多媒体教学课件的过程. 相似文献
114.
Savlík M Polácková L Szotáková B Lamka J Velík J Skálová L 《Research in veterinary science》2007,83(1):20-26
Basal activities of certain pheasant hepatic and intestinal biotransformation enzymes and modulation of their activities by anthelmintics flubendazole (FLBZ) and mebendazole (MBZ) were investigated in subcellular fractions that were prepared from liver and small intestine of control and FLBZ or MBZ treated birds. Several oxidation, reduction and conjugation enzyme activities were assessed. In the liver, treatment of pheasants by FLBZ or MBZ caused very slight or no changes in monooxygenase activities and conjugation enzymes. More significative changes were detected in small intestine. Metyrapone and daunorubicin reductase activities were increased by both substances in the liver. This is the first evidence that certain benzimidazoles modulate reductases of carbonyl group. With respect to the relatively slight extent of the changes caused by FLBZ or MBZ we can assume that repeated administration of therapeutic doses of both FLBZ and MBZ has probably no serious influence on pheasant biotransformation enzyme system. 相似文献
115.
AIM: To investigate the effects of gonadotropin-releasing hormone (GnRH) analogue on the growth of breast cancer cell lines MCF-7 and MDA-MB-231 in vitro and to explore the related mechanisms with PI3K/Akt or ERK/MAPK pathways. METHODS: The proliferation of human breast cancer cell lines MCF-7 and MDA-MB-231 treatment with triptorelin was detected by MTT assay and the distribution of the cell cycle was determined by flow cytometry. The phosphorylation of the ERK1/2 and Akt was evaluated by Western blotting. RESULTS: Triptorelin inhibited the proliferation of MCF-7 cells at concentration of 10-5 mol/L after treated for 192 h or at concentration of 10-4 mol/L after treated for 168 h and 192 h. Triptorelin inhibited the proliferation of MDA-MB-231 cells at concentration of 10-4 mol/L after treated for 192 h (P<0.05).Treatment with triptorelin for 192 h at concentration of 10-4 mol/L had no statistical significance effect on phosphorylation of ERK1/2 and Akt(P>0.05).CONCLUSION: Inhibitory effect of GnRH analogue triptorelin on human breast cancer cells is not just the connection with the down-regulation of pituitary hormone, but also a direct inhibitory effect. The role may not be involved in the activation of ERK/MAPK and PI3K/Akt signaling pathways. 相似文献
116.
117.
小麦7D染色体数量性状基因定位和效应估计的研究 总被引:10,自引:0,他引:10
综合应用方差分析法、区间定位法和联合定位法将控制抽穗期、有效穗数、小穗数、50粒重和单穗产量等5个性状的6个数量性状基因座(QTL)定位在小麦7D染色体上。其中控制粒重的QTL有2个。这些QTL集中分布在着丝粒附近和染色体长臂的末端两个区域。效应估计结果表明多数QTL的效应是微效的,控制同一个性状(粒重)的两个QTL的效应几乎相等。比较3种QTL定位法的分析结果,方差分析简单有效,且当染色体上只有 相似文献
118.
Z.Y. Xin Z.Y. Zhang X. Chen Z.S. Lin Y.Z. Ma H.J. Xu P.M. Banks P.J. Larkin 《Euphytica》2001,119(1-2):163-167
We developed some wheat-Th. intermedium translocation lines,Yw642, Yw443 and Yw243, etc., showing good BYDV resistance from L1by induced homoeologous pairing using CS ph mutant. Characterization ofthese wheat lines was carried out by GISH and RFLP analysis. The resultsof GISH showed that the lines, YWw42, Yw443 and Yw243, etc., arehomozygous wheat-Th. intermedium translocation lines, in which thechromosome segments of Th. intermedium were transferred to thedistal end of a pair of wheat chromosomes. RFLP analysis indicated that thetranslocation chromosome of the wheat lines is T7DS · 7DL-7XL. Thebreakpoint of the translocation is located on the distal end of 7DL, betweenXpsr965 and Xpsr680 about 90–99 cm from the centromere. The BYDVgene is located on the distal end of 7XL around Xpsr680, Xpsr687 andXwg380. The RFLP markers of psr680, psr687 and wg380 werecosegregated with the BYDV resistance respectively and could be used formolecular assisted selection (MAS) in wheat breeding program for BYDVresistance. 相似文献
119.
Molecular marker analysis can be an effective tool when searching for new fire blight resistance donors. It can speed up the breeding process as well, even though many of the available markers linked to fire blight resistance QTLs have not yet been tested by screening a large number of cultivars. The aim of this study was to search for alternate sources of the three major QTLs of fire blight resistance; FBF7, FB_MR5 and FB_E, as well as to test the efficiency of some markers linked to minor QTLs. Altogether, nine primer pairs were used on 77 genotypes including new Hungarian cultivars and old apple cultivars from the Carpathian basin. Several marker alleles of FB resistance QTLs have been detected in the screened genotypes, most importantly the alleles coupling with FB_MR5 in the old cultivars ‘Kéresi muskotály’, ‘Szabadkai szercsika’ and ‘Batul’. We propose these cultivars as the first available resistance donors of FB_MR5 instead of the crabapple Malus × robusta 5. The results also bring new information regarding the resistance alleles of new Hungarian cultivars and selections. 相似文献
120.
Ricardo Augusto Diniz Cabral Ferreira Magno Antonio Patto Ramalho Fernando Henrique Ribeiro Barrozo Toledo Angela de Fátima Barbosa Abreu 《Plant Breeding》2015,134(5):599-604
In the common bean crop in Brazil, the requirement of the value for cultivation and use trials is that these experiments must be conducted over two years in three locations per region. Information in regard to the necessary number of years to ensure precision in cultivar recommendation and the influence of evaluated years in the estimation of the GE interaction are still scarce. Using grain yield of five check varieties assessed over 11 years in three sowing seasons per year, the aims of this study are as follows: to measure the relative contribution of the GE interaction, evaluating the implication of the number of years in the estimates of the GE interaction, and infer how many years are needed to ensure precision in cultivar recommendation. For instances, analysis of variance was carried out involving all environments and also combinations of years. The results showed that the GE interaction was greater than all other cross‐effects involving lines. The use of at least two years allows good coincidence in cultivar recommendation compared to the whole period. Increasing the evaluation time is a good strategy, especially when it is difficult to grow three different sowing seasons. 相似文献