试述结合场地特征的景观设计——以山西大同“桐城怡景”为例

场地特征是场地传达出来的信息,包括空间上的自然特征和时间上的人文特征。通过分析大同"桐城怡景"景观,探讨场地特征对于设计的重要意义,并为该场地找寻出合适的设计语言。     

农村产业结构协调发展的探索——山西省孝义市柱濮镇农村产业状况剖析

农业是属于社会效益高、比较经济效益低的特殊产业。在社会主义市场经济条件下，如何深化对农业基础地位的认识，按照经济发展的客观规律，科学地指导农业和农村经济持续、快速、健康地发展，尤其是在工矿业发达的地区，如何扭转工业发展、农业萎缩的局面，使工农业相互促进，协调发展，是农业经济工作者研究的一个重要课题。本文分析了孝义市柱濮镇农村产业现状及其农业发展滞后的原因，提出了农村产业协调发展的对策、建议     

高速公路沥青路面结构设计

沥青路面结构设计是保证高速公路沥青路面施工及使用质量的关键，本文介绍了临长高速公路沥青路面结构层厚度设计，为同类设计提供参考。     

犬冠状病毒的分离鉴定及其纤突蛋白基因的克隆与序列比较

以犬胎原代细胞，采用同步接种、带毒传代和添加新细胞的方法，从１例腹泻犬脾脏和１例临床健康犬肠内容物中分离出２株犬冠状病毒（ＣＣＶＹＳ１，ＣＣＶ ＣＩ１）。该２株病毒可使ＣＲＦＫ细胞出现典型的细胞融合病变，与从美国引进的ＣＣＶＮＬ－１８参考株形成的细胞病变相似；电镜负染和超薄切片观察，分离毒株细胞培养物中均含有典型的冠状病毒粒子，并形成胞浆内包涵体；经理化学、生物学鉴定，濉有ＣＣＶ的特性；间接免疫荧     

新型木材削片机的结构特点

从传统削片机存在的缺点和不足的角度入手，概述了当今国外削片机为提高削片质量、减少冲击和振动、提高削片效率所采取的措施和方法，并分析了新型削片机的结构特点。     

马铃薯A病毒(PVA)运动相关蛋白质的研究进展

综述了马铃薯A病毒(PVA)运动相关蛋白的结构、功能方面的研究进展.     

A contribution to the systematics of a piedmontese plum ecotype

Prunus domestica L., P. insititia L., P. salicina Lindl. and P. cerasifera Ehrh. are the most important species included in the plum group. Many ‘cultivars’ have an undefined origin and share very similar morpho‐phenological characteristics that make their identification difficult. This is the case of ‘Ramasin’, a group of Piedmontese genotypes whose fruits are small, ellipsoidal and very tasty. They are commonly considered as P. domestica varieties, even though on the basis of some morphological characteristics they are similar to other species such as P. insititia. In order to study the origin of this group, the DNA of 25 genotypes of ‘Ramasin’, and that of some ‘cultivars’ of P. domestica, P. insititia, P. cerasifera, P. salicina and two selections of P. spinosa L. was examined. The PCR‐RAPDs method (Random Amplified Polymorphic DNA amplified by Polymerase Chain Reaction) was used and all 69 primers that were tested generated more or less polymorphic bands. From the results of these analyses it can be supposed that the ‘Ramasin’ plums are a single genetically heterogeneous group. Moreover it can probably be assumed that ‘Ramasin’ arose from crosses between P. domestica and P. insititia.     

Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond,and its relationships with agronomic traits

Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient method for producing DNA fingerprints and molecular characterization. Our objectives were to: estimate genetic similarities (GS), marker indices, and polymorphic information contents (PICs) for AFLP markers in almond cultivars; assess the genetic diversity of almond cultivars and wild species, using GS estimated from AFLP fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression and cultivar improvement. The genetic diversity of 45 almond cultivars from Iran, Europe, and America, were studied assaying 19 primer combinations. In addition, several agronomic traits were evaluated, including flowering and maturity times, self-incompatibility, and kernel and fruit properties. Out of the 813 polymerase chain reaction fragments that were scored, 781 (96.23%) were polymorphic. GS ranged from 0.5 to 0.96, marker indices ranged from 51.37 to 78.79, and PICs ranged from 0.56 to 0.86. Results allowed the unique molecular identification of all assayed genotypes. However, the correlation between genetic similarity clustering as based on AFLP and clustering for agronomic traits was low. Cluster analysis based on AFLP data clearly differentiated the genotypes and wild species according to their origin and pedigree, whereas, cluster analysis based on agronomic data differentiated according the pomological characterization. Our results showed the great genetic diversity of the almond cultivars and their interest for almond breeding.     

水稻(Oryza sativa)新型液泡Na+/H+逆向转运蛋白基因的特征分析和表达

以拟南芥AtNHX1 cDNA 片段作为探针，筛查水稻盐胁迫植株叶片cDNA 文库，获得与AtNHX1同源的水稻新型液泡Na⁺/H⁺逆向转运蛋白基因（OsANT1）。序列分析表明，OsANT1 全长cDNA为2 178 bp，包括一个长度为1 608 bp的完整开放阅读框，编码535个氨基酸残基。在DNA水平上，OsANT1基因含有15个外显子和14个内含子，长度为4 835 bp。OsANT1含有12个跨膜域，系统进化树分析结果表明，与来自拟南芥、水稻、小麦、玉米、大麦、马蔺和芦苇等的Na⁺/H⁺逆向转运蛋白高度同源。盐胁迫条件下，OsANT1的表达具有盐分诱导特征，且随着胁迫的增大而增加。表明该基因可能在水稻抵御盐分胁迫的过程中具有一定作用。