苣荬菜染色体特征研究

苣荬菜(Sonchus arvensis L.)属菊科苣荬菜属,是一种野生药食两用植物.采用低温处理法处理苣荬菜种子根尖,对苣荬菜染色体特征进行定量分析.结果表明:苣荬菜的体细胞染色体数目为18条,并得出其核型公式为K(2n)=2x=10m+ 8sm,核型分类属于2A型.研究结果将为苣荬菜的细胞遗传学研究提供基础依据.     

Characterization and classification of phytoplasmas from wild and cultivated plants by RFLP and sequence analysis of ribosomal DNA

Restriction fragment length polymorphism and sequence analysis of PCR-amplified ribosomal DNA were used to identify and classify phytoplasmas associated with diseases of various wild and cultivated plants. The diseases examined were either not known before or the presumable causal agents were not yet identified and characterized or were only known from other geographic areas. New diseases examined were those causing virescence and phyllody of Bunias orientalis and Cardaria draba. Both were associated with strains of the aster yellows phytoplasma. The same type of aster yellows phytoplasma was also found to be associated with yellows and phyllody diseases of Portulaca oleracea, Stellaria media, Daucus carota ssp. sativus, and Cyclamen persicum. In German and French DNA samples from diseased Trifolium repens, the clover phyllody phytoplasma was identified, which could clearly be distinguished from other phytoplasmas of the aster yellows group. Strains of the stolbur phytoplasma were detected in big bud-affected tomatoes and almost exclusively in Convolvulus arvensis. In Cirsium arvense and Picris echioides two distinct phytoplasmas were identified which showed relationship to the sugarcane white leaf phytoplasma group but may represent a new group or subgroup. In Conyza (syn.: Erigeron) canadensis a phytoplasma of the X-disease group was detected. A strain from Gossypium hirsutum showed the same restriction profiles as the faba bean phyllody phytoplasma.     

Factors in the suppression of weeds by squash interplanted in corn

In order to examine the weed-suppressive properties of squash interplanted in corn, we compared different planting densities and weeding regimens, measuring the weed biomass, light interception by crop canopy, and yield. The corn was machine-planted at a normal monocrop density, while the squash was hand-planted at or above a normal monocrop density in an irrigated and otherwise mechanized system. A simulated intercrop consisting of corn with an artificial squash canopy was included. Interplanted squash reduced the biomass of the total weeds and of the dominant weeds, Amaranthus retroflexus and Convolvulus arvensis . The corn yield was not necessarily reduced in the intercrop, except at a higher squash density or under limited moisture. Shading by the squash was the major mechanism of weed suppression, but the analysis suggested that other factors, such as allelopathy, might contribute. Additional hand weeding had no effect on the corn yield.     

Variation Between Barley Cultivars in Early Response to Weed Competition

An experiment was conducted with three spring barley ( Hordeum vulgare ssp. vulgare L.) cultivars of contrasting competitiveness to identify variations in their early competitive response to weed competition and to study canopy architecture and growth development at the early stages of cultivar growth. The aim was also to investigate whether differences in shoot morphology, growth development and competitive response could explain the differences in competitive ability against weeds. The barley cultivars were grown outdoors in boxes either in monoculture or in a mixture with white mustard ( Sinapis alba L.) as a model weed. The experiment was run until the barley cultivars were in the stem elongation stage. The varieties with strong to medium competitive ability against weeds shortened the time of emergence in the presence of S. alba in contrast to the least competitive cultivar. The results also indicated that spring barley cultivars with strong competitive ability against weeds have an early stem extension as a response to weed competition and a low competitive response. Morphological traits, namely large length of the two first internodes, long main shoot in the tillering stage and a small leaf angle, may be important traits in competition for light.     

Successful backcrosses of somatic hybrids between Sinapis alba and Brassica oleracea with the Brassica oleracea parent

Somatic hybrids between Sinapis alba (2n= 24) and Brassica oleracea (2n= 18) have been backcrossed with the B. oleracea parent. Whereas backcrosses with the diploid B. oleracea parent were unsuccessful, 344 BC₁ seeds could be obtained from inter-valence crosses with tetraploid B. oleracea (2n= 4x= 36). The investigated 96 BC₁ plants segregated for morphological traits and for fertility. They were backcrossed with diploid B. oleracea or self-pollinated, depending on their male fertility. The BC₁F₂ and BC₂ progenies segregated well for the morphological traits. Disturbances were observed especially in the generative phase (flower development and pollen fertility). Both male fertile and male sterile BC₁F₂ and BC₂ plants were obtained and backcrossed or self-pollinated with the B. oleracea parent. The presence of either one of the parental or the cybrid organelle genomes was detected. In the progenies, a stable maternal inheritance of the organelle genome patterns was observed. Isozyme analyses revealed polymorphism for the leucine aminopeptidase (LAP) which was used for the identification of S. alba genes in the progenies. Cytological investigations showed a clear differentiation between the BC₁F₂ and BC₂ plants. Whereas the BC₁F₂ plants possess large numbers of chromosomes ranging from 34 to 40, the BC₂ material was strongly reduced to chromosome numbers ranging from 20 to 22. Preliminary investigation of the meiosis suggests the possibility of introgressions of S. alba-DNA into the B. oleracea genome.     

Production of intergeneric hybrids between Raphanm and Moricandia

Intergeneric F₁ hybrids between Raphanus sativus (2n = 18, RR) and Moricandia arvensis (2n = 28, MaMa) have been produced through ovary culture followed by embryo culture, when M. arvensis was used as a pistillate parent. Six BC₁ plants were also obtained through ovary culture followed by embryo culture in the backcross of an amphidiploid F₁, hybrid with R. sativus cv. 'Pink ball'. Two BC₁ plants were ses-quidiploids (2n = 32, MaRR), and the other BC₁, plants were hyperploid with 2n = 55, having MaMaRRR genomes. BC₂, seeds were obtained by conventional pollination in the successive backcross of two sesquidiploid BC₁, plants with R. sativus cv. 'Pink ball'. Their seed set percentages were 12.7% and 17.0%, respectively. These novel hybrid plants and derived progenies may be valuable materials for the genetic investigation and breeding of Brassiceae , including R. sativus.     

An evaluation of the potential of intergeneric gene transfer between Brassica napus and Sinapis arvensis

The possibility of gene transfer between Brassica napus and Sinapis arvensis was evaluated. Six spring-type cultivars of B. napus and four strains of S. arvensis were reciprocally crossed through controlled crosses. No hybrid was yielded from any cross. However, one hybrid with 28 chromosomes was obtained from B. napus×S. arvensis through ovule culture. The hybrid plant was highly sterile and set no seed on open pollination. Two F₂ plants, with 35 and 36 chromosomes respectively, were obtained through self-pollination by hand. Backcross of B. napus produced 23 plants carrying some characteristics of S. arvensis, but backcross to S. arvensis failed to produce a plant. The chromosome counts of the BC₁F₁ plants indicated that gametes with more than nine chromosomes were favoured during the meiosis. The data demonstrated that gene transfer from S. arvensis to B. napus was very difficult under controlled cross and backcross, while to transfer genes from B. napus to S. arvensis would be extremely remote even under the most favorable conditions.     

Evaluation of alternate menthol mint (Mentha arvensis L.) based intensive cropping systems for Indo-Gangetic plains of north India

The objective of the study was to determine the profitability and employment-generation potential of different cropping systems involving menthol mint (Mentha arvensis L.) as a component of sequential/intercropping in comparison with the most common paddy–wheat–green gram cropping system. Field experiments were conducted at Lucknow, India (26° 5′ N, 80° 5′ E and 120 m above mean sea level) for three years from July 2004 to June 2007. Menthol mint yielded the maximum fresh shoot biomass and essential oil (21.0 t and 151 kg ha^?1, respectively) grown after sweet basil (Ocimum basillicum)–potato followed by paddy–potato–menthol mint (18.9 t and 136 kg ha^?1, respectively) and maize–mustard–menthol mint (17.7 t and 131 kg ha^?1, respectively). Net returns of all the menthol-mint-based cropping systems were 82.6–354% higher than traditional paddy–wheat–green gram cropping system. Maize–garlic–menthol mint + okra was found to be most profitable (77,200 Rs ha^?1) followed by pigeon pea + sweet basil–menthol mint + okra (76,120 Rs ha^?1). Employment-generation efficiency was much higher in cropping systems involving menthol mint and vegetable crops, the highest (2.21 man days ha^?1 day^?1) being in a maize–cauliflower–onion–menthol mint + okra cropping system.     

Changes of weed infestation under long-term effect of different soil pH levels and amount of phosphorus:potassium

ABSTRACT

The aim of the present study was to investigate changes of weed species density as a result of long-term (1976–2005) exposure to different soil pH levels, and P₂O₅:K₂O amount in soil created by initial and subsequent periodical liming, and fertilization. As a result of liming during the period 1976–2005, average soil pH levels at the start of the sixth crop rotation (2001–2005) ranged from 4.1 (unlimed) to 6.6. And in each pH plot were four sub-plots with a different amount of mobile phosphorus:potassium. In acid soil (pH 4.1), in spring oilseed rape and in spring barley crops, the dominant weed Spergula arvensis density decreased significantly at a pH of 5.1 or higher. With increasing amount of P₂O₅:K₂O in soil, the abundance of S. arvensis in spring rape and spring barley crops was reduced at all soil pH levels. At pH 5.1, Chenopodium album and Tripleurospermum perforatum were prominent. Elytrigia repens tended to decrease with increased alkalinity and nutrient amount in soil. With reduction of soil acidity from pH 4.1 to 6.6, the total weed infestation consistently declined in all crops. A low amount of P₂O₅:K₂O in soil caused the decline in the abundance of S. arvensis, E. repens and increase in C. album and T. perforatum.     

Production and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica oleracea

Somatic hybrids were produced between Moricandia arvensis (MaMa, 2n= 28) and Brassica oleracea (CC, 2n= 18) through cell fusion and then characterized by analysing their morphology, cytology, DNA constitution, leaf anatomy and seed fertility. Cell fusion was carried out between greenish protoplasts isolated from the mesophyll of M. arvensis and colourless ones from hypocotyls of B. oleracea. Three plants were generated from one shoot via cuttings and acclimatized in vivo. They closely resembled each other in morphology, exhibiting traits intermediate between the parental species. They were confirmed to be amphidiploids by mitotic and meiotic analyses, being 2n= 46 (MaMaCC), with pollen fertility of about 50%, which was enough to develop the subsequent progenies. Anatomical analysis of the for leaf tissue showed that the bundle sheath cells of the somatic hybrids contained some centripetally arranged organelles, like those of M. arvensis. The hybridity was also confirmed by random amplified polymorphic DNA analysis. Both chloroplast DNA and mitochondrial DNA of the somatic hybrids were estimated to be derived from M. arvensis. In leaf anatomy, the somatic hybrid showed the C₃‐C₄ intermediate trait as in M. arvensis. Many progenies resulted from backcrossing with parental species. The somatic hybrids are expected to be used as bridging plant material to introduce the C₃‐C₄ intermediate trait into Brassica crop species.