香榧茎段离体培养再生植株的研究

对香榧茎段不定芽诱导研究的结果表明,不定芽诱导的最佳培养条件为:培养基为B5+KT0.1mg/L+IBA0.5mg/L+0.08%活性炭+2%葡萄糖,培养温度为20℃,光强不高于400lx,该条件下的不定芽诱导率可达55%。将不定芽培养在1/2B5+IBA0.1mg/L+0.08%活性炭+2%葡萄糖的生根培养基上,生根率最高达到40%。通过器官发生途径国内外首次建立了香榧离体再生体系和快繁体系,并获得了完整的再生植株,为今后开展香榧的遗传转化和品种改良奠定了基础。     

竹果氨基酸种类与含量的分析

本文对所收集的5簇11属15种竹果的氨基酸含量进行了分析测定,其结果为: Ⅰ.从测定的15个种的竹果来看,均含有18种蛋白质水解的氨基酸,其中均以谷氨酸的含量为最高,其余各种氨基酸的含量比例各不相同。Ⅱ.氨基酸总量各种之间差别很大,含量最高的是冷箭竹为18.4448g/100g,最低的是巴山木竹6.33g/100g。Ⅲ.不同类型竹果的氨基酸总含量几乎没有什么太大的差别,而同类型中不同种之间的差别较大。Ⅳ.各种氨基酸在不同的属之间所含的量和比例不同,但在同一属里,含量和比例具有比较明显一致的曲线峰。Ⅴ.各属种之间,各种氨基酸占总氨基酸的含量比例相当接近。Ⅵ.竹果与笋体所含氨基酸在种类上大体相同,而在含量上无论是各分量或总量,竹果都大大超过了笋体。氨基酸含量的多少,对于竹类种子的萌发、生长、抗性等都具有一定的影响。这一内容的研究,为进一步探讨竹类各属种之间的生物学特性提供了一定的科学依据。     

抗生素对火炬松的器官发生植株再生的影响(英文)

本文研究了氨苄青霉素、羧苄青霉素和头孢霉素等3种抗生素对火炬松愈伤组织的生长和分化及不定芽生根的影响。结果表明,头孢霉素最有利于愈伤组织的诱导和生长,羧苄青霉素最有利于芽的分化,氨苄青霉素降低了不定芽的生根频率。所有试验的3种抗生素提高愈伤组织的形成和芽再生,但降低了芽的生根频率。这些结果表明,选择合适的抗生素对优化火炬松遗传转化体系有重要作用。图3表4参25。     

优良笋用竹种引种试验总结报告

1987年冬和1988年冬二次从浙江引种六个笋用竹种1.56万株,成活率达90％以上,发笋率达80％以上,新竹的秆基粗度已达到母竹的粗度,引种是成功的。本文分析了各引种环节的技术效果。     

Variation in Shoot Anatomy and Rooting Behaviour of Stem Cuttings in Relation to Age of Donor Plants in Teak (Tectona grandis Linn. f.)

Shoot anatomical features viz., number of vessel elements, width and length of vessel and fibre elements were studied in relation to juvenility/maturity of teak (Tectona grandis Linn. f.). The findings show significant differences in these features, which indicates that variation in shoot anatomy can be used as a reliable indicator of juvenility/maturity in teak. The effect of age of donor plants and application of auxins on adventitious root formation in single noded leafy stem cuttings of teak was also investigated. Aging of donor trees suppressed rooting and sprouting of cuttings, but increased callus formation at the base of cuttings. Per cent rooting and per cent sprouting in cuttings decreased as age of donor plants increased from 2-months to 15-years and up to 30-years. IBA treatment promoted per cent rooting as well as per cent sprouting, NAA promoted only rooting and it suppressed sprouting. The effect of auxin treatment on rooting and sprouting of teak stem cuttings varied with the age of donor plants. Auxin and its concentration, which suppressed rooting and sprouting in cuttings of younger donor plants, actually promoted rooting when cuttings were taken from more mature donor plants. It appears that the auxin requirement for causing and promoting rooting in teak cuttings increased with increasing age of donor plants. The higher auxin requirement for causing and promoting rooting in cuttings of older trees may be due to a decrease in the content on endogenous auxins or decreased sensitivity of aging tissues to rooting promoters and /or accumulation of inhibitory substances which inhibit rooting.     

云南东南部天然沙罗竹林分结构规律的研究

沙罗竹为大中型丛生竹类,生长较好的天然竹林主要集中分布在云南河口——屏边大围山海拔500 m地区的沟谷地带,面积约5 000 hm2,具有很高的开发利用价值。该文对其林分的密度、直径、生物量等因子变化规律及其相互关系首次进行了系统研究。     

Foliar and shoot allometry of pollarded black locust, Robinia pseudoacacia L.

Browse of multipurpose tree species such as black locust could be used to broaden grazing options, but the temporal distribution of foliage has not been adequately studied. Our objective was to determine effects of harvest date, P fertilization (0 and 600 kg ha⁻¹ yr⁻¹), and pollard height (shoots clipped at 5-, 50-, and 100-cm above ground) on foliar and shoot allometry of black locust. The experiment was conducted on a naturally regenerated 2-yr-old black locust stand (15,000 trees ha⁻¹). Basal shoot diameter and foliar mass were measured monthly in June to October 2002 and 2003. Foliar and shoot dry mass (Y) was estimated from basal shoot diameter (D) by the function Y = aD ^b , with regression explaining ≥95% of variance. Allometry of foliar mass was affected by harvest date, increasing at a greater rate with D in September than in June or July, but not by P fertilization or pollard height. Foliar mass was predicted best by month-specific equations for the June to October growth interval. Allometry of shoot dry mass was unaffected by harvest date, P fertilization, or pollard height. These equations could be used as a first approximation of foliar and shoot mass for pollarded black locust.     

枣树绿枝扦插试验研究

本试验选用当年生半木质化枣头发育枝作扦插材料，用不同激素、不同浓度进行处理，以河沙做扦插基质；用塑料小拱棚和人工洒水调控温湿度；用竹帘搭棚遮荫简单行的方法，使枣树绿枝扦插当年成活率达８５％以上。冬季采用覆麦草越冬。     

福建酸竹鲜笋采后防腐保鲜剂研制

本次实验结果显示福建酸竹笋发霉的致病菌为青霉、曲霉和枝孢霉。通过抑菌实验可得出最佳的复合防腐配方为B13B233%食盐。在确定保鲜配方的生笋实验中,得出福建酸竹生笋保鲜液的最佳配方为复配防腐液(A 0.08? 0.05? 3%食盐县pH=4.5) 0.03? 0.03?,该配方经省检疫局检测LD50>20m l.kg-1(比重1.043),几乎无毒,属于对人体安全保鲜液。用此复配防腐液处理福建酸竹笋在贮藏27天时还能保持可食性,保鲜效果明显,具有很大的推广前景。