玫瑰黄链霉菌Men myco 93 63抗南方根结线虫相关酶活性及其防效

根据雌虫会阴花纹特征将采集地河北省定州市西南合村黄瓜棚的根结线虫鉴定为南方根结线虫,并研究对多种植物病原菌具有较强抑制作用的玫瑰黄链霉菌Men myco 93 63对南方根结线虫的防效及其产生的与防治线虫相关的酶。结果表明,Men myco 93 63发酵液对根结线虫二龄幼虫的校正致死率达到100%,粗酶液对线虫的校正致死率达到94.87%。Men myco 93 63具有较强的胶原蛋白酶产生能力,也可产生丝氨酸蛋白酶和蛋白酶,发酵液中蛋白酶的活力达到473.363 7 U/mL。推测胶原蛋白酶、蛋白酶、丝氨酸蛋白酶等酶类在该生防菌对南方根结线虫的防治中起重要作用。温室盆栽试验结果表明,玫瑰黄链霉菌Men myco 93 63发酵液及其固体发酵物对根结线虫均有防效;其中,发酵液5倍稀释液对根结线虫的防效最好,达到39.99%。     

花生新品种汕油188和汕油199品比试验

为筛选适宜博罗县推广种植的优良花生新品种,替代逐年退化的当家品种,2011年用从汕头市农业科学研究所引进汕油188、汕油199两个花生新品种与博罗县花生当家品种粤油7号进行品种比较试验,结果表明:汕油188、汕油199产量均比粤油7号高,且农艺性状和经济性状、抗病性较强,可进一步示范种植.     

斜纹夜蛾核型多角体病毒Ⅱ型分离株ORF63基因的序列特征及表达与启动子活性分析

斜纹夜蛾核型多角体病毒Ⅱ型(SpltMNPVⅡ)分离株是繁殖率和毒力极强的新型病毒株,ORF63是该病毒分离株的一个功能未知基因。从SpltMNPVⅡ分离株基因组中克隆了ORF63。序列分析表明,该基因读码框为1 071 bp,编码356个氨基酸,蛋白质分子质量为41.3 kD;起始密码子ATG上游存在一个早期和晚期启动子基序,编码蛋白序列含有CNX、MutH等多种结构域。启动子活性和转录时相分析表明ORF63是一个早、晚期表达的基因,在病毒感染4 h和18 h时有2个转录峰,24 h以后转录水平略有下降,但总体趋于稳定。构建该基因的原核表达载体pET-28a-ORF63,并转化大肠杆菌BL21(DE3),表达并纯化融合蛋白后制备多克隆抗体,Western blot检测制备的多克隆抗体特异性较好,效价可达1∶3 200以上。由以上结果推测,SpltMNPVⅡ分离株的ORF63基因是一个早期和晚期均有表达的病毒基因,可能与SpltMNPVⅡ感染宿主细胞后自身DNA的复制有关,参与早期芽生病毒(BV)的发生和晚期包涵体衍生病毒(ODV)的成熟2个过程。     

Adenocarcinoma of the parotid salivary gland in a cow

A 6‐year‐old Girolando dairy cow was presented for evaluation of a large subcutaneous facial mass. Fine‐needle aspirates of the mass contained many neoplastic cells with high nuclear:cytoplasmic ratios arranged in sheets and loosely cohesive clusters with streaming erythrocytes and neutrophils in the background. Neoplastic cells were 13–25 μm in diameter and were round to cuboidal with variably distinct borders. Based on the signalment, anatomic location, and cytologic findings, differential diagnoses included salivary adenocarcinoma, squamous cell carcinoma, and mucoepidermoid carcinoma. The cow was euthanized and a necropsy was performed. The primary neoplasm arose from the left parotid salivary gland and meastatic tumor was found in the regional lymph nodes and lung. Histologically, the tumor was composed of anastomosing and irregular solid islets surrounded by scant stroma. Cells were negative for periodic acid‐Schiff (PAS), PAS‐diastase, and Alcian blue pH 2.5 stains, used to detect mucin. On immunohistochemical analysis, neoplastic luminal salivary gland cells expressed cytokeratin, but not S100, α‐smooth muscle actin, or vimentin. Peripheral cells of neoplastic islets were immunoreactive for p63. The final diagnosis was nonsecretory adenocarcinoma of the parotid salivary gland.     

玫瑰黄链霉菌抗药性标记及其定殖和促生作用研究

选用腐霉利、多菌灵、甲基托布津、利福平、青霉素、链霉素、泰乐菌素、重铬酸钾等8种常用抗生素或杀菌剂,通过抗药性标记试验,建立了玫瑰黄链霉菌(Stremptomyces roseoflavus)Men-myco-93-63的抗药性标记,确定了重铬酸钾(0.06g/L)/青霉素(0.2g/L)为适合检测该菌株的标记药剂及浓度。通过获得的抗药性标记,结合菌落形态观察和16SrDNA序列分析,研究了Men-myco-93-63在番茄根际土壤中的定殖动态和对植株生长的影响。结果表明,该生防菌在番茄根际土壤中能够定殖,接菌15d后定殖量达到最高,为5.6×105 cfu/g,之后下降,到30d后检测不到Men-myco-93-63菌株的存在。当使用Men-myco-93-63的固体发酵物与土壤以体积比1∶400混合时,对番茄苗期生长有促进作用。     

Development of a New Excellent Cytoplasmic Type of CMS Line in Rice

A new excellent cytoplasmic type of CMS line Sl0-2A in rice with typical abortion type (TAT)from Minghui 63 was obtained by in vitro culture. It possesses high percentage of exerted stigma with strong vitality, big opening angle of spikelet, high flowering rate before noon and iow percentage of cleistogamous spikelet. The relationship between restoring and maintaining of S10-2A is completely different from that of WA type, HL type and BT type.     

汕优63生育中后期碳水化合物的转运及分布规律研究

用~(14)C示踪,探索中稻汕优63生育中后期碳水化合物的转运及分布规律,结果表明,不同生育期标记同化的~(14)C产物,各期测定均以穗部分配最多,茎内次之,鞘内再次,芽内最少。     

皖稻42号(中粳63)的选育及栽培技术

概述了皖稻４２号（中粳６３）的选育经过、特征特性、试种示范表现及其栽培技术。     

Relationship between physicochemical characteristics of Korean wheat flour and quality attributes of steamed bread

The purpose of this study is to identify major factors affecting the manufacture and quality of steamed bread, consumed in Southeast Asia including China, Japan, and Korea. Hence, flours of 11 Korean wheat cultivars were used to evaluate quality attributes of two different styles of steamed bread, Korean style steamed bread (KSSB) and northern-style Chinese steamed bread (NSCSB). KSSB prepared more ingredients and higher optimum water absorption of dough than NSCSB because Korean consumers prefer white and glossy surface and soft crumb. KSSB showed lower height, larger diameter and volume of steamed bread, higher stress relaxation, and softer texture of crumb than NSCSB. The correlation between flour characteristics and quality of steamed bread was different in KSSB and NSCSB. About 90% of variability in the height and volume of KSSB could be predicted from protein content, mixing tolerance of Mixograph, average particle size of flour, final viscosity and solvent retention capacity. Protein content and quality parameters also could explain the variation of steamed bread height in NSCSB. Korean wheat carrying Glu-A3c allele produced higher volume of steamed bread (704.7 mL) than Glu-A3d allele (645.8 mL) in KSSB, although there was no significant difference in volume of NSCSB by glutenin compositions. Glu-D1d and Glu-A3c alleles had softer texture of crumb than Glu-D1f and Glu-A3d alleles in KSSB, Glu-B3i allele also showed lower hardness of crumb than their counterpart allele in NSCSB. Hard wheat showed higher height and volume of steamed bread, and lower stress relaxation and hardness of crumb than soft wheat in KSSB.