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71.
Tetsuro?Shiraishi Kohei?Ohta Akihiko?Yamaguchi Mari?Yoda Hisashi?Chuda Michiya?MatsuyamaEmail author 《Fisheries Science》2005,71(3):531-542
ABSTRACT: Final oocyte maturation and ovulation of captive chub mackerel Scomber japonicus with fully yolk-accumulated oocytes were induced by a single injection of human chorionic gonadotropin. Reproductive parameters, including spawning frequency and batch fecundity, which are required to estimate spawning biomass in pelagic fish by the daily egg production method, were analyzed. Germinal vesicle migration (GVM) occurred at 18–24 h post-injection, and the hydration and ovulation of oocytes were completed at 30 and 36 h post-injection, respectively. The results of the maturation process suggest that fish with GVM-stage ovaries captured in the daytime from the field are capable of spawning on the night following their capture. The oocytes used in the oocyte size-frequency distribution method for batch fecundity estimates should be at late GVM and more advanced stages. The results of sequential artificial insemination showed that the quality of ovulated eggs held in the ovarian lumen rapidly deteriorated as time progressed after ovulation. This indicates that the fertilization window for the ovulated eggs of chub mackerel lasts only a few hours, and spawning behavior should be performed within a few hours after ovulation in the wild population. 相似文献
72.
M. Matsuyama K. Ohta S. Morita M.M. Hoque H. Kagawa A. Kambegawa 《Fish physiology and biochemistry》1998,19(1):1-11
The female bambooleaf wrasse, Pseudolabrus japonicus, spawns daily during the spawning season, and exhibits a diurnal rhythm of ovarian development. In the present study, we have investigated: (1) circulating levels of 17a, 20-dihydroxy-4-pregnen- 17,20-P) and 17,20,21-trihydroxy-4-pregnen-3-one (20-S) in females sampled at different times of the day during spawning season in captivity, and (2) in vitro production of 17,20-P and 20-S by follicle-enclosed oocytes at seven different develo tal stages. In addition, we developed a microtiter plate enzyme-linked immunosorbent assay (ELISA) for 17,20-P. Serum levels of 17,20-P and 20-S showed similar diurnal changes; substantial increases in these levels occurred around the time of germinal vesicle breakdown (GVBD). In vitro experiments showed that massive production of 17,20-P and 20-S occurred in follicles collected just before or during GVBD. Further, acute decreases in 17,20-P and 20-S production were found in the ovarian follicles just prior to ovulation, suggesting inactivation of the maturation-inducing hormone (MIH). These results, taken together with our previous data on the occurrence of GVBD in vitro, suggest a role for both 17,20-P and 20b-S as MIHs in the bambooleaf wrasse. 相似文献
73.
74.
To determine how stock abundance fluctuations of walleye pollock Theragra chalcogramma are related to variations in reproductive characteristics, the length at 50% maturity of the fish sampled off the Pacific
coast of northern Japan from 1990 to 1999 was examined. In both sexes, the fish density increased, and the body length of
age-3-5 fish decreased, but the condition factor and the age at 50% maturity showed no clear trend during this period. Male
length at 50% maturity decreased, while value for females showed no clear trend. In both sexes, significant negative correlations
were found between density index (combined age-3-5) and each body length at age-3-5. Significant positive correlations were
seen between each body length at age-3-5 and length at 50% maturity in males. Positive correlation between body length at
age-5 and length at 50% maturity was significant in females from 1990 to 1999, except for the samples in 1998. We suggest
that the fish density might affect the length at maturity in males and possibly also in females. 相似文献
75.
76.
为了检验发情牛(Bos taurus)血清在猪卵母细胞成熟中的应用,实验分别在成熟培养液中加入不同的血清,观察卵母细胞的成熟效果,并通过孤雌激活胚胎和重构胚的体外发育能力来判断血清对卵母细胞质量的影响:研究还进行了用发情牛血清成熟的卵母细胞构建的核移植胚胎的体内发育试验.结果表明,发情牛血清,成年牛血清和胎牛血清在猪卵母细胞的成熟率方面没有显著差异(P>0.05);卵母细胞成熟时加入不同的血清,对卵母细胞体外发育能力的影响不显著(P>0.05);来自含发情牛血清成熟液培养的卵母细胞,与胎儿成纤维细胞构建的克隆胚胎能够完成全期发育. 相似文献
77.
78.
79.
Bielanski A. Simard C. Maxwell P. Nadin-Davis S. 《Veterinary research communications》2001,25(8):663-673
The association of bovine immunodeficiency virus (BIV) with embryos derived by in vitro fertilization from oocytes of experimentally infected heifers or oocytes/embryos exposed to the virus in vitro was investigated. Using a nested-PCR assay, proviral DNA of BIV was not detected in follicular fluid or in embryos derived from BIV-infected donors. In vitro exposure of oocytes to BIV during maturation or insemination with BIV-infected semen resulted in zona pellucida-intact embryos testing negative for BIV provirus. However, exposure of zona pellucida-free day-7 embryos to the virus resulted in a positive BIV assay for 28% of the batches of embryos, suggesting that the zona pellucida has a role in protecting against BIV infection. The presence of BIV in the IVF system had no apparent effect on the development of bovine embryos to the blastocyst stage. 相似文献
80.
将不同直径牛卵泡内卵母细胞体外成熟培养22h后,对其体外受精后的卵裂率,胚胎发育速度和胚胎染色体异常发生率进行了研究。结果表明,体外受精后48h,直径3-6mm卵泡内卵母细胞外受精后的卵裂率明显高于直径1-2mm和7-10mm卵泡内卵母细胞体外受精后的卵裂率(P<0.05)。体外受精后48h,直径3-6mm包泡内卵母细胞体外受精后的胚胎的发育速度明显快于直径1-2mm和7-10mm卵泡组胚胎(P<0.05)。通过对不同卵裂期胚胎的染色体标本分析表明,直径1-2mm卵泡内卵母细胞体外受精后的胚胎染色体异常发生率明显高于直径3-6mm卵泡组(P<0.05),但与直径7-10mm卵泡组之间无显著性差异。 相似文献