马铃薯大西洋品种脱毒种薯生产栽培技术

本文主要从以下几点,即品种生物学特性;种薯选择;栽培技术;田间管理和适时收获系统地阐述了大西洋品种的栽培技术,并为大西洋品种大面积种薯生产提供参考。     

沙松造林技术的研究

对沙松造林技术的研究结果表明，最适宜沙松生长的条件是：造林密度为６６００株／ｈｍ＾２；山下部，阴坡半阴坡；整地方式为边整边造。     

照明效果及照度标准在设计中的应用

现代电气照明是一门技术与艺术相结合的学科。照明设计作为照明技术成果的体现,是完成各种照明设施的手段,它不仅涉及照明理论,光源研究、照明器研制、建筑设计及色彩调配、灯光分布等工程内容,更强调照明生理学及照明心理学。故在设计中应力求达到上述要求,并注意整体效果及艺术效果。     

银纹夜蛾人工饲养技术研究

本文在研究了各种环境因子对银纹夜蛾实验种群影响的基础上，进一步研究了食量，低温保种及饲养管理技术，设计了一种便于收集卵的新型产卵笼，并总结了人工饲养银纹夜蛾的技术要点。     

One-step RT-PCR for the Detection of Infectious Bursal Disease Virus in Clinical Samples

A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA.     

山丹马6-磷酸葡萄糖脱氢酶及葡萄糖磷酸异构酶的多态性研究

用淀粉凝胶电泳及琼脂覆盖技术对山丹马的血液红细胞6磷酸葡萄糖脱氢酶和葡萄糖磷酸异构酶的电泳变异进行了测定。在6磷酸葡萄糖脱氢酶座位发现了3种表现型,即FF,FD和FS,其频率分别为0.958,0.021和0.021。在葡萄糖磷酸异构酶座位发现了两种表现型,即II和FI,其频率分别为0.771和0.229。等位基因频率直接通过表型计算出:PGDF为0.979,PGDD为0.0104,PGDS为0.0104;GPIF为0.1146,GPII为0.8854。亲子关系排除概率在6PGD和GPI座位点分别为0.1009和0.0912。两座位的个体识别概率分别为0.081和0.353。     

Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation: Dynamics of inoculum

Using field plots where rhizomania had not previously been detected, different inoculum levels of beet necrotic yellow vein virus (BNYVV) were created by application of infested soil. A susceptible sugar beet cultivar (cv. Regina) was grown for two consecutive years (1988 and 1989), in the presence or absence of drip irrigation. In soil samples taken in spring 1989, the different initial inoculum levels of 1988 could be distinguished using a quantitative bioassay estimating most probable numbers (MPNs) of infective units per 100 g dry soil. The first sugar beet crop resulted in a tenthousandfold multiplication of inoculum of BNYVV (viruliferousPolymyxa betae). Mean MPNs of BNYVV ranged from 0.6 and 7 per 100 g soil for the lowest inoculum level to 630 and 1100 per 100 g for the highest level, in plots without and with irrigation, respectively. In spring 1990, MPNs had again increased. In both years, the initial inoculum level of 1988 had a significant linear effect on log-transformed MPNs of BNYVV determined. Log-transformed MPNs for 1990 and 1989 showed a positive linear correlation, despite a decreasing multiplication ratio at higher inoculum levels. Drip irrigation during one or two years enhanced the increase in MPN of BNYVV, which was reflected by the enhancement of multiplication ratios at all inoculum levels. The totalP. betae population was also higher after growing two irrigated crops than after growing two non-irrigated ones.     

鄂北地区栗瘿蜂综合防治技术研究

栗瘿蜂在鄂北地区一年发生一代，以初龄幼虫在被害芽内越冬。翌年4月上旬栗芽萌动时开始为害，使被害芽形成虫瘿抑制新梢生长，导致减产甚至绝收。当地该瘿蜂优势种天敌是中华长尾小蜂，益害比1∶5时能抑制栗瘿蜂的大发生。以冬季清园铲除杂草，剪除病虫枝等农业防治为基础，收集虫瘿于3月下旬挂于栗园保护天敌为主导，辅之以化学防治的综合防治措施能有效地控制栗瘿蜂的为害。     

不同工艺饲用基质对奶牛生产性能的影响

通过不同生物发酵工艺研究食用菌培养基质开发生态饲料的生产工艺。经对不同工艺食用菌培养基质营养成分和卫生安全主要指标检测及饲养试验筛选，以“活力菌＋糖＋米糠＋水”组合半固体糖化微生物发酵处理工艺效果较明显，发酵时间以7～15d为宜。粗蛋白和粗脂肪分别为14．8％和6．03％，比发酵前增加3．13％和5．12％。粗纤维26．57％，比发酵前降低5．5％。奶牛试验设4个工艺，20％饲用基质试验组与对照组，该工艺组奶料比最高，比对照组高0．56％。     

西瓜枯萎病菌遗传多样性的相关序列扩增多态性分析

对30个西瓜枯萎病菌Fusarium oxysporum f.sp.niveum菌株基因组DNA进行相关序列扩增多态性(SRAP)分子标记分析,以探究其遗传多样性与地理来源的关系。采用尖孢镰刀菌西瓜专化型Fusarium oxysporumf.sp.niveum0、1、2号生理小种的基因组DNA为模板,对225对SRAP引物进行筛选,筛选出20对多态性、重复性较好且条带清晰的引物,对30个菌株进行PCR扩增,共扩增出386条带,其中多态性条带有371条,多态性比率为96.11%,平均每对引物扩增出19.3个位点和18.55个多态性位点。UPGMA法聚类分析结果显示,供试菌株两两之间的遗传相似系数范围为0.69~0.90,平均为0.79,说明尖孢镰刀菌西瓜专化型的遗传多样性较为丰富。基于SRAP标记聚类分析表明,30个菌株在遗传相似系数为0.70处被划分为3个类群,I类群包含24个菌株,其中18个来自湖南省,Ⅱ类群只包含1个来自黑龙江省哈尔滨市的菌株,它和另一个来自黑龙江地区的菌株被划分到不同的类群,且遗传距离相对较远;Ⅲ类群包含了5个菌株,其中3个来自海南三亚,其余两个来自湖南省。根据菌株的分布情况来看,菌株的聚集与地理来源没有明显的相关性。