改革开放以来甘肃省产业结构效益分析

根据1978～2008年有关甘肃省三次产业的时间序列数据,在产业结构效益分析方法综述的基础上,应用比较劳动生产率、产业结构偏离度和偏离-份额分析模型,从不同角度对改革开放以来甘肃产业结构效益进行了研究。结果表明,甘肃第一、三产业比较劳动生产率较小,而第二产业较大;产业结构偏差系数较大,产业结构与就业结构极不对称;朝阳产业比重较大,对经济增长的贡献较大,但三次产业竞争力都相对较弱,有待进一步提高。     

1951—2009年乌鲁木齐市气候变化特征分析

利用乌鲁木齐市1951—2009年的地面观测资料,分析了近20年和近10年以来气候变化,并提出了建议,以供参考。     

In vivo and in vitro protein digestibility in juvenile bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840) fed soybean meal‐based diets

In vivo and in vitro protein digestibility of 0% to 60% soybean meal protein (SBM) substitution from fishmeal protein was conducted for bagrid catfish Mystus nemurus juveniles. Seven experimental diets containing 35% protein and 15% lipid were fed to bagrid catfish to determine the in vivo protein digestibility. In vitro methods were determined using pH stat, pH shift, spectrophotometric assay and sodium dodecyle sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) with different enzyme mixtures (crude intestinal extract of bagrid catfish, Lazo 1‐enzyme, Hsu 3‐enzyme, Saterlee 4‐enzyme). In vivo and in vitro protein digestibility decreased with increased SBM protein substitution with highest tolerance level of 10% (in vivo). pH stat and pH drop methods showed the highest degree of hydrolysis (DH) and relative protein digestibility (RPD) using Saterlee 4‐enzyme system. However, pH stat method showed highest correlations (r² = 0.9263) with the in vivo results using crude intestinal enzyme extract compared to other enzyme systems. The highest correlation of the in vitro methods using crude intestinal enzyme extracts of bagrid catfish was determined using spectrophotometric assay (r² = 0.9284) followed by pH stat (r² = 0.9263), SDS‐PAGE (r² = 0.8348) and pH drop (r² = 0.6777). All the in vitro methods tested are suitable to rapidly determine protein digestibility for bagrid catfish except for pH drop.     

北京山区刺槐林净初级生产力对气候变化的响应

利用BIOME—BGC模型估算北京山区刺槐林的净初级生产力(NPP),并分析了NPP对不同未来气候情景的响应。结果表明:模型模拟出的NPP总体上低于样地实测值,年际变动率为11.2%,并表现出波浪形年际变化规律;降水量与NPP呈现极显著正相关关系,是NPP年际变化的主要气候因子;CO2的浓度加倍、降水增加对NPP积累起到促进作用,温度增加不利于NPP的积累;降水增加对NPP积累的正向效应不敌温度增加对NPP积累的负向作用,CO2浓度加倍、降水增加对NPP积累的协同促进作用大于温度增加对NPP积累的抑制作用。     

厢式沉沙池进口优化试验及流场三维数值模拟

通过对沉沙池原设计方案模型试验发现,原设计方案沉沙池存在着三池厢流量分配不均,进口流速及水面波动较大等缺陷.通过模型试验发现,在整流前池中设置整流横梁等措施后,沉沙池中水流流态得到了较大改善,三池厢出口流量分配及流速分布趋于均匀.在此基础上,采用数学模型对沉沙池中流态进行了三维数值模拟,得到了沉沙池中完整的流场信息,结果表明,计算结果与试验观测资料吻合良好,弥补了试验观测资料不足的缺陷.     

小麦苗期叶片中性蛋白水解酶酶谱的动态变化

本试验采用复性电泳技术对小麦菌期蛋白水解酶种类及活性进行了动态研究。结果表明：（1）小麦从出苗第3d生长到第87d，153，72和68kD三条蛋白水解酶带均存在；（2）72和68kD两条蛋白水解酶带在各个时期均表现出很强的活性；（3）小麦的生理代谢影响着蛋白水解酶谱变化；（4）所检到的蛋白水解酶分子量均大于68kD(含68kD).     

New disease symptoms observed on field-grown potato plants with potato spindle tuber viroid and potato virus Y infections

Summary Potato spindle tuber viroid (PSTV) and potato virus Y (PVY) were isolated from plants of cultivar Kennebec with severe necrotic symptoms in the field. In the greenhouse, severe necrotic symptoms were reproduced only when potato plants were infected either simultaneously with PSTV+PVY, or with PSTV prior to PVY infection. Thirteen additional potato cultivars were tested in the greenhouse for this synergistic reaction and eight developed necrotic responses similar to cv. Kennebec. PVY concentration was significantly higher in doubly infected plants, compared with those infected with PVY alone.     

小麦高分子量谷蛋白亚基近等基因系的研究

以安农8455为母本,Pan555为父本组合杂交,并采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法(SDS PAGE)对安农8455/Pan555的620粒F2籽粒的高分子量麦谷蛋白亚基(HMW GS)进行了测定,结果表明:F2籽粒中共出现了15种带型组合,可稳定出多对近等基因系;Glu A1a与Glu A1c分离比为1∶1;Glu B1b与Glu B1c分离比为9∶7;Glu D1d与Glu D1i分离比为1∶1;Glu B1b与或Glu B1c的不完全表达率为4 0%。     

聚丙烯酰胺电泳条带中微量DNA片段的回收与再扩增方法的改进

【目的】研究聚丙烯酰胺凝胶电泳(PAGE)条带中微量DNA片段的回收和再扩增.【方法】比较了4种方法溶解聚丙烯酰胺凝胶的效果和回收微量核酸的效率.【结果和结论】Bioteke溶胶/结合液能获得极高的DNA片段回收率,可从仅含1.0~7.5 ng核酸的PAGE胶中有效回收到足量的片段;再扩增的效果与回收效率呈正相关;将液态混合液与杂质分离是成功进行再扩增的关键.本研究构建的一套快速、高效的PAGE多态性条带回收及再扩增的方法为分析生物遗传多样性成因提供了新途径.