植物激素对丽格海棠组织培养快繁的影响

根据目前丽格海棠组织培养快繁现状,研究了以MS培养基为基础附加不同种类和配比植物激素对丽格海棠组织培养中不定芽再生、不定芽继代增殖和生根的影响。结果表明:丽格海棠不定芽再生的最佳植物激素配比为MS+1mg/L6-BA+0.1mg/LTDZ或MS+1mg/L6-BA+0.1mg/L2.4-D;不定芽继代增殖的最佳植物激素配比为MS+2mg/L6-BA+0~0.1mg/LNAA;组培苗生根的最佳植物激素配比为MS+0.1~0.3mg/LIBA。     

激素对黄花苜蓿扦插成活率及生长的影响

以现蕾期黄花苜蓿的新鲜茎节为扦插条,研究不同激素对黄花苜蓿扦插成活率及生长的影响。结果表明,3种激素处理后黄花苜蓿扦插成活率依次为吲丁萘乙酸>硝钠萘乙酸>萘乙酸,且浓度为1 200 mg/L的吲丁萘乙酸激素处理扦插成活率高达90.83%,且生根率为100%。     

红实美草莓试管苗瓶外生根的研究

以红实美草莓试管苗为材料,研究了试管苗大小、基质、环境条件对试管苗瓶外生根的影响,并与瓶内生根相比较。试验结果表明,在温度23~27℃、湿度85%、光照为半遮光条件下7d后,3.0~4.0cm大小的试管苗在草炭∶煤渣(1∶1)基质中瓶外生根效果最好,生根移栽成活率98.6%,平均生根数7.8条/株;与瓶内生根相比,不仅降低了生产成本,而且还能缩短快繁时间28d。     

不同继代培养次数对苹果八棱海棠离体培养和遗传转化的影响

以苹果八棱海棠离体新梢和幼叶为外植体,研究不同继代次数对其离体繁殖、再生和遗传转化的影响。结果表明,随着继代培养次数的增加,继代10次和48次的八棱海棠离体增殖倍数、生根能力、不定芽的再生频率、每外植体再生芽数和GUS基因瞬时表达率均显著高于继代5次的;继代10次的增殖倍数和GUS阳性率明显多于继代48次的,其它指标相互间差异不显著;说明继代10次的芽苗较适合用于八棱海棠离体培养的外殖体材料,其幼叶较适于遗传转化研究。     

Growth, survival, and root system morphology of deeply planted Corylus colurna 7 years after transplanting and the effects of root collar excavation

Urban trees are frequently planted with their root collars and structural roots buried well below soil grade, either because of planting practices, nursery production practices, or both. These deeply planted structural roots can impair tree establishment and are thought to reduce tree growth, lifespan, and stability, although research has provided few and contradictory results on these questions to date. This study examines container-grown (55 L) Turkish hazel trees (Corylus colurna L.), planted either at grade, 15 cm below grade, or 30 cm below grade into a well-drained silt loam soil, over nearly 8 years. Five years after planting, in 2004, remediation treatments (root collar excavations) were performed on two replicates of each below-ground treatment. Subsequently, all trees were subjected to flooding stress by being irrigated to soil saturation for approximately 6 weeks. In 2006, flooding stress was repeated. Trees root systems were partially excavated in 2007, and root architecture was characterized. Deep planting did not affect trunk diameter growth over 8 years. Survival was 100% for the first 5 years; however, one 30 cm below grade tree died after flooding in 2004 and another died after the 2006 flooding. Photosynthesis was monitored during the 2004 flooding and all trees experienced decline in photosynthetic rates. There was an apparent slight delay in the decline for trees with excavated root collars and those planted at grade. Girdling roots reduced trunk taper and occurred primarily on unremediated trees planted 30 cm below grade.

Selected individual roots were excavated and followed from the root ball and were observed to gradually rise to the upper soil regions. Analysis of roots emerging from excavation trench faces indicated that vertical root distribution at approximately 1.25 m from the tree trunks was the same regardless of planting depth. Longterm consequences of planting below grade are discussed.     

草石蚕扦插繁殖技术研究

[目的]为草石蚕的科学栽培提供依据。[方法]分别在5月20日和10月15日取草石蚕绿苗期的幼嫩枝条进行扦插育苗,观察幼苗生根和生长情况并计算成活率及繁殖系数,寻找其扦插繁殖的最佳环境条件。[结果]在春季,草石蚕的扦插苗在20d后即可生根,生根率达95%,生根后成活率为100%,而繁殖系数达到23.75。而秋季扦插苗的生根率只有10%左右,繁殖系数为2.5。春季扦插苗在整个生育过程中生长良好,能正常越冬,次年正常萌发。[结论]草石蚕的扦插繁殖以春季(5月20日左右)为宜。     

ABT生根粉提高报春花种子活力的研究

为 提高报 春花种子 活力和幼 苗的抗 逆性 , 以 Ａ Ｂ Ｔ 生根 粉 不同 剂型 、不 同浓 度浸 泡 处理报春花 种子, 进 行了播种 发芽试 验。结果 表明 , 经 Ａ Ｂ Ｔ 生 根粉 浸 泡处 理 后, 发 芽率 、发 芽速率、发 芽指数、 活力指数 等活力指 标均明 显提高, 以６ 号 Ａ Ｂ Ｔ 生根 粉２０ ｍｇ Ｌ 浸泡２４ｈ 的处理效果最 佳。电导 率值和干 鲜比低 于 Ｃ Ｋ, 与种子 活力存在 负相关 关系。     

美洲黑杨×青杨F_1无性系生根性状的遗传变异及C效应

通过水培生根试验,对１２个美洲黑杨×青杨Ｆ１无性系及两亲本的最早生根时间、主根总长、主根数、侧根数等生根特性进行了研究。所有生根性状均存在显著或极显著的无性系差异,无性系方差占总方差的１４％７０％～３９６６％,并具有较强的超亲优势;以无性系平均为基准的广义遗传力均较高,选择生根能力好的无性系是有效的。所有生根性状均存在极显著的Ｃ效应,Ｃ效应方差占总方差的１３７２％～２０８１％,在进行早期无性系选择时应引起重视。     

激素和基质对黄花远志扦插生根的影响试验

激素和扦插基质是影响扦插生根的重要因素.本试验研究不同激素和扦插基质对黄花远志扦插生根的影响.试验开始于2004年4月15日,7月26日出苗,并统计生根情况.结果表明插条的生根率与激素浓度有关,生根质量与激素的种类有关;插条在400mg·L-1吲哚丁酸十400mg·L-1萘乙酸、800mg/L吲哚丁酸、800mg·L-1萘乙酸溶液中浸泡10min,生根效果较好,生根率分别达到34.0%、29.3%、28.8%;蛭石+珍珠岩、泥炭土+河沙两种扦插基质相比,生根效果以泥炭土+河沙为佳.     

大果黑果枸杞组培快繁技术体系研究

[目的]该研究旨在建立大果黑果枸杞组培快繁技术体系。[方法]以大果黑果枸杞嫩茎作为外植体,以 MS为基本培养基,研究了不同因素对大果黑果枸杞组培苗初代、继代以及生根培养的影响。[结果]适合大果黑果枸杞初代培养的培养基配方为 MS+ZT 0.2 mg/L+IBA 0.01 mg/L,在此培养基上,腋芽的生长状况良好,萌芽率高达88.73%,且玻璃化的情况很少;同样, ZT对组培苗增殖培养的效果要优于6-BA,适合组培苗增殖培养的培养基配方为MS+ZT 0.15 mg/L+ IBA 0.01 mg/L,在此培养基上,组培苗的生长速度快,其增殖倍数可达5.83倍,组培苗基本没有玻璃化现象,且苗生长健壮;适合组培苗生根的培养基为 MS+IBA1.0 mg/L,生根率可达100%。[结论]适合大果黑果枸杞组培苗炼苗移栽的基质配比为腐殖土∶珍珠岩=1∶1,在此基质中培养的苗木均长势良好,成活率高达92.37%。[结论]该研究为大果黑果枸杞的规模化生产及推广提供理论依据。