红王子锦带的嫩枝扦插繁育研究

利用快速生根粉、吲哚乙酸、G G R 3种生根剂对红王子锦带插穗进行处理,结果表明,以G G R的100 m g/L溶液进行60 m in生根处理的红王子锦带生根率最高,达92%。嫩枝扦插的繁殖方式可节约成本,缩短生长时间,达到较高的经济效益。     

欧李嫩枝扦插多因素比较试验

对欧李嫩枝扦插关键因素的比较试验结果表明，欧李嫩枝扦播必须带叶；最佳繁育期在5月中旬；ABT1号生根粉浓度在50～100mg／L时，可提高生根率20％以上。     

观赏向日葵丛生芽增殖、生根及离体成花

本文以观赏向日葵品种‘富阳’为供试材料，通过MS基本培养基添加不同植物生长调节剂试验，初步建立了适宜观赏向日葵无菌苗增殖、生根和离体成花培养条件。结果表明，MS＋低浓度BA对无菌苗增殖效果较佳，丛生芽增殖最适培养基为MS＋0．05mg／LBA；无菌苗生根率受营养水平影响，最适生根培养基为1／2MS；在离体条件下，低浓度BA可延缓植株衰老促进开花，最适开花培养基为MS＋0．05mg／LBA。花芽分化受GA3和PP333所抑制。本研究可为观赏向日葵遗传转化体系建立及转基因植株性状鉴定提供基础。     

红肉苹果的组织培养快繁技术

[目的]建立红肉苹果的高效植株再生体系。【方法]以红肉苹果的茎尖和带腋芽的茎段为外植体，通过组织培养试验筛选出最佳的灭菌时间、基础培养基、增殖培养基和生根培养基。[结果]随着消毒时间的延长，外植体的污染率明显下降，但外植体受到的伤害显著增加。用0．1％HgCl2灭菌5rain的外植体生长正常，污染率仅为3．3％。在增殖培养基MS＋6一BA0．5mg／L＋NAA0．1mg／L、MS＋6．BA0．5mg／L＋NAA0．2mg／L和MS＋6一BA0．5mg／L＋NAA0．5mg／L中。每个外植体平均增殖不定芽4．1、6．8和3．3个。在生根培养基1／2MS＋0．1mg／LIBA＋0．1mg／LNAA、1／2MS＋0．2mg／LIBA＋0．2mg／LN从和1／2MS＋0．5mg／LIBA＋0．5mg／LNAA上幼苗的生根率分别为56．7％、96．7％和83．3％。[结论]该研究为红肉苹果的快速离体扩繁和工厂化育苗以及园林应用奠定了良好的基础。     

无核白鸡心葡萄的高空压条生根试验

为了给葡萄的高空压条繁殖提供参考,以3年生无核白鸡心葡萄苗为材料,研究不同浓度的生长调节剂萘乙酸(NAA)和不同包被材料(园土、基质)对压条生根的影响。结果表明:NAA对葡萄高空压条生根具有明显的促进作用,且以400 mg/L的生根效果最好,包被材料用基质最佳。     

满天星生根培养的研究

以满天星小花型品种‘仙女’作为试验材料 ,在MS培养基上加不同浓度的PP333和NAA组合 ,将不定芽平放或直立于培养基上进行培养 ,研究其生根情况。结果表明 :在所有NAA和PP333组合的培养基上 ,平放的不定芽生根速度、生根量和生根率均高于直立的不定芽 ;外源激素浓度以NAA 0 .0 1mg·L- 1 PP3330 .10mg·L- 1为最佳。     

Effect of auxin treatments,cuttings’ collection date and initial characteristics on Paeonia ‘Yang Fei Chu Yu’ cutting propagation

Paeonia ‘Yang Fei Chu Yu’ is one of the most popular and commercially valuable cultivars of herbaceous peony. The study was performed to explore propagation techniques by cuttings for the nursery industry. Results showed that the stem cuttings pretreated with 2000 mg L⁻¹ indole-3-butyric acid (IBA) in quick-dip method got the best rooting traits (rooting percentage is 86.7%, root number is 23.1 and root length per rooted cutting is 6.4 cm). Therefore pre-treatment with 2000 mg L⁻¹ IBA is recommended.     

‘Swingle’ citrumelo propagation by cuttings for citrus nursery tree production or inarching

‘Swingle’ citrumelo [Citrus paradisi MacFaden × Poncirus trifoliata (L.) Raf.] has been extensively used as a rootstock in several citrus growing regions of the World, including Southern Brazil where ‘Rangpur’ lime (Citrus limonia Osbeck) is still the predominant variety despite being affected by several important pathogens. In this case, ‘Swingle’ citrumelo is used to produce nursery trees to establish new orchards or to be inarched in adult and healthy groves in order to change the rootstock. We report herein a system to produce trees on ‘Swingle’ citrumelo more rapidly by budding onto non-rooted cuttings, as well as assessing potential to rapidly multiply ‘Swingle’ through rooting of non-budded cuttings. Therefore, two potential products are described: budded trees and rooted rootstock cuttings. ‘Valencia’ sweet orange [Citrus sinensis (L.) Osbeck] was budded at different heights on cuttings derived from eight-month old rootstocks. Grafted and additional non-budded cuttings were then treated with indole-3-butyric acid (500 mg L⁻¹) or left untreated before rooting. Three types of cuttings were evaluated: softwood, semi-hardwood and hardwood. The use of nursery trees derived from pre-budded hardwood cuttings of ‘Swingle’ citrumelo is an alternative grafting method on this cultivar. Softwood cuttings with one leaf pair were considered the most adequate material for rapid multiplication of ‘Swingle’ citrumelo by cutting. This could be particularly useful for inarching production or conventional budding after transplant of cutting-derived rootstocks.     

菊花‘绿鹦哥’的组织培养和快速繁殖

以菊花'绿鹦哥'茎段、叶盘为外植体,选用MS培养基为基本培养基,附加激素6-BA和NAA,研究不同激素浓度组合对外植体愈伤诱导及不定芽分化的影响.结果表明:菊花'绿鹦哥'茎段最适芽诱导分化培养基分别为MS+6-BA 2 mg/L+NAA 0.1 mg/L;小苗的最佳生根培养基为1/2 MS+NAA 0.1 mg/L,生根率可达100%.     

植物生长调节剂对月季扦插生根的影响

以“超级玫瑰”为材料,研究了不同浓度的NAA和IBA对扦插月季愈伤组织和根发生的影响。结果表明:以浓度为150mg/LIBA溶液处理的效果最好;NAA和IBA对月季愈伤组织和根发生都有显著性促进作用,在本试验条件下,两者的促进作用没有显著性差异。