联合收割机的二次切割分向输送部件研究

概述了二次切割分向输送部件的国内外研究动态，提出了该作业方式的设计依据，介绍了立式和卧式二次切割分向输送部件的研究实例，理论分析和试验表明，采用此类部件是提高联合收割机生产率和性能的一个重要方法。     

沼肥对水稻增产作用的研究

本文根据本所4年沼肥肥效定位试验结果,证实沼气发酵剩余物是一种缓速兼性的优质有机肥料。不仅沼液、浮渣、沉渣单独施用对水稻有较为显著的增产作用,而且沼液、浮渣、沉渣合并施用对水稻的增产作用更为显著,因此,必须纠正某些地方“弃渣用液”或“弃液用渣”的片面施用沼肥的现象,大力提倡全沼肥的合理施用。     

基于计算机视觉的大米外观品质检测

开发了一套基于计算机视觉技术的稻谷品质检测系统，采用灰度变换、自动阈值分割、区域标记等方法从采集的稻米群体图像中提取单体米粒图像，对单体米粒的裂纹、垩白特征进行了统计和检测方法研究。提取了米粒的面积、周长等10个特征参数作为整精米检测特征，并进行了主成分分析，确定了判别整精米的优化阈值。检测试验结果表明：裂纹米粒识别的准确率为96．41％；垩白米粒识别的准确率为94．79％；整精米识别的准确率为96.20%。     

超级稻联合收获机具的探讨

每公顷产量10500kg超级杂交稻已经问世．超级杂交稻与现有水稻的特性差别很大，现有推广使用的联合收割机都不适应超级稻的收获，且穗头上方有大量直立叶片．半喂人机型根本无法适应，因此有必要尽早研究开发新型的联合收割机．以适应即将大面积推广的超级稻的收获。     

温度对不同育成地水稻品种的生长及其物质生产的影响

以原产温带的日本晴三粳稻品种和原产热带的IR8两籼稻品种为材料,观察了16—30℃恒温对其生长和物质生产的影响。结果:在试验温度范围内,各个品种的植株均随温度升高而增高;但两类品种的株高感温指数有差异,IR8两品种的较低,日本晴三品种的较高。IR8两品种在30℃下分蘖数最大,日本晴三品种则是在25℃下,较有利于它们的分蘖。IR8两品种的CGR和NAR,以30℃下最大,日本晴三品种则以25℃下的最大,干物质生产量亦呈此趋势。所有供试品种都是在25℃下出叶最少,出叶间隔天数则以在20℃下为最快。     

辽宁省2005年审定水稻品种米质情况分析及改良对策

以2005年辽宁省审定的32个水稻品种为材料,对辽宁省水稻品种品质近况进行了分析。在所有的材料中,6项指标全部达到国标一级标准的有3个,达标率为9.38%;达标率最高的是糙米率,其次是胶稠度、整精米率,最低的是直链淀粉含量。同时对辽宁省水稻品质改良中需要着重解决的几个问题进行了探讨。     

Improvement of Appearance Traits of elite Rice Zhenshan 97B by Molecular Introgression of Targeted about 800 kb Interval from Indica Rice Jiafuzhan Genome

Rice appearance trait is mainly mean grain length (GL), grain width (GW), ratio of length and width (L/W), chalkiness (CH) and transparence (TR). Appearance is the first impression, and rice appearance traits effect significantly the value of commodity. Hybrid rice is relatively low quality, especially on rice appearance quality,which restricts partially its extend and badly export of hybrid seed and rice in China.Shanyou 63, the most famous hybrid rice combination of China, could not be also sustainable application because of its poor qualities. In this studies, We attempt to improve appearance quality of the elite rice maintainer of Zhenshan 97B by using molecular approach of genomic interval targeted introgression. Three F2 populations were constructed to study the classical and molecular genetic of rice appearance traits, at the same time, molecular marker-asisted toimprove the main maintainer line, Zhenshan 97B were performed. There some following results:Three F2 populations were constructed based on crosses of Guangluai 4×Jiafuzhan (GJ popu.), Minghui 86×Jiafuzhan (MJ popu.) and Zhenshan 97B×Jiafuzhan (ZJ popu.). ZJ popu and GJ popu were produced to reconstruct the linkage maps related with rice appearance traits based on BSA, and detect QTL of target traits based on IM.In ZJ popu, 9 QTLs of target traits were detected. The interval, RM169～RM516 on Chromosome 5, was genetic locus of GW, L/W, CH and BW, and the explained variances were 10.9%, 14.9%, 12.0% and 14.2%, respectively.RM214 on chromosome 7, RM339 on chromosome 8 were genetic loci if CH and BW, and the explained variances were 9.4%, 10.0%, 11.0% and 12.1%. besides there is a genetic locus, RM347, of G/W on chromosome 3, and the explained varance was 10.3%. In GJ popu, 5 QTLs of rice appearance traits were detected. The interval, RM169-RM516 on Chromosome 5, were also the genetic loci of GW, CH and BW, and the explained variances were 9.0%, 12.5%, and 13.6%, respectively. RM264～RM80 on chromosome 8 was the genetic loci of GW amd L/W,and the explained variances were 14.7% and 13.4%. The genotypes of all QTLs, except RM214, from Jiafuzhan contributed to improving riee appearance traits.The research was to improve the rice appearance quality of Zhenshan 97B by introgressing QTLs of rice appearance traits from Jiafuzhan, a donor parent, by means of MAS in the process of recurrent backcrossing, 4 SSR markers were used to detect the target genotypes from donor parent, and 70 SSR primers to identify the genetic background of recurrent parent. Consequently, there were 19 target individuals in BC3, in which the mean value of GL, GW, L/W, CH and BW were 6.06±0.04mm, 2.50±0.02mm, 2.43±0.02, 29.5±2.7 and 29.4±2.7%, respectively,and the mean homologous degree (HD) to recurrent parent is up to 98.22%. Compared to Zhenshan 97B, GL, CH and BW decreased 0.17 mm, 34.9% and 34.5% respectively and however L/W increased 0.19. which accounted separately for 25.37%, 54.79%, 54.57% and 10.50% of thedifference of every trait between two parents. It is very luckly to find a individual, 13BC2-3BC3 line, whose GL, GW, G/W, CH and BW were 6.04 mm, 2.43 mm, 2.49,2.5% and 2.5% separately, There is very important signification for it to be used in hybrid rice breeding improving appearance traits. In the research, MAS efficiency of 3 loci (with 4 markers ) was 5.68% and coincident degree was 73.08%.     

Improving Drought Tolerance of Rice by Designed QTL Pyramiding

Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem, we developed a new strategy ＇designed QTL pyramiding＇ to more efficiently develop drought tolerant （DT） rice cultivars. In the first phase of this strategy, we developed large numbers of introgression lines （ILs） in elite backgrounds using backcross （BC） breeding, each of which carries multiple genomic segments for improved DT from a known donor. Then, we genotyped all ILs with SSR markers to track the genomewide pattern of introgression in the DT ILs of 16 BC populations derived from crosses between 2 recipients （IR64 and Teqing） and four different donors. X^2 tests and linkage disequilibrium analyses of introgression in the DT ILs revealed significant frequency shifts at many loci across the genome and non-random associations at the multilocus level as a result of selection for DT in the ILs, which led us to the discovery of putative genetic networks underlying DT in the ILs. The networks each containing all DT loci detected in ILs from a BC population showed some interesting features.     

OsI2基因的克隆及其植物表达载体的构建

在旱稻IRAT109干旱胁迫下cDNA芯片分析结果的基础上,通过RT-PCR,5'-RACE及3'-RACE方法,从IRAT109总RNA中扩增得到了干旱胁迫下芯片表达谱中上升表达强度第二的基因全长序列,命名为OsI2,全长有523 bp,并对基因序列结构进行了分析.该基因与全长cDNA文库中的CT836140.1有99%同源.OsI2基因编码产物对应1个包含57个氨基酸的开放阅读框(ORF),为一功能未知蛋白.其编码产物也可能对应两个中间相隔19 bp的较小ORFs(43个氨基酸和42个氨基酸),都是功能未知的蛋白.在pBI121载体的基础上,将OsI2基因与CaMV35S连接成功构建了pBl121-OsI2植物表达载体,为进一步研究其功能创造了条件.     

温度影响下的稻纵卷叶螟实验种群动态的系统动力学模拟

温度是影响稻纵卷叶螟（Cnaphalocrocis medinalis Guenee）发育、存活和繁殖的关键生态因子之一。研究温度与稻纵卷叶螟种群数量变动的关系,可为害虫种群的控制提供科学依据。本文根据稻纵卷叶螟在不同温度处理下（19.0℃,22.0℃,25.0℃,28.0℃,31.0℃）的实验种群生命表,利用系统动力学的相关理论和方法构建仿真模型,对稻纵卷叶螟实验种群的数量动态进行了模拟,在此基础上预测成虫的产卵量,并通过敏感性分析确定了成虫产卵的最适温度。结果显示,在初始卵量均为100标准粒的情况下,温度过高（〉25.0℃）不利于稻纵卷叶螟实验种群低龄幼虫的生存和发育,温度过低或过高均不利于实验种群后期的发育和繁殖;随着温度的上升,稻纵卷叶螟的存活率和生殖力迅速提高,温度为25.0℃时世代存活率、次代产卵量达到最高值,分别为19.67%和1488.4头;稻纵卷叶螟成虫的最高产卵量出现在24.9℃。研究表明了系统动力学模型在害虫种群发育动态及预测方面有着良好的应用潜力。