青蒿B-BOX型锌指蛋白基因AaBBX22的克隆及表达分析

在外界环境刺激下BBOX型锌指蛋白基因往往可以诱导植物相关基因的应答反应抵抗逆境胁迫。为了研究青蒿中AaBBX22基因在逆境胁迫中的表达特点,我们从青蒿的cDNA文库中克隆得到了AaBBX22基因。该基因全长为1114bp,包含1个813bp的开放阅读框。生物信息学分析表明该基因具有2个典型的＆BOX型锌离子结合位点。进化树分析显示AaBBX22与葡萄和大豆的B-BOX型基N具有较近的亲缘关系。实时荧光定量PCR结果表明,在正常生长条件下,该基因在青蒿的各个组织部位中都有表达,其在根中的表达量最高,在花蕾中的表达量最低。盐和干旱处理都能显著诱导该基因的表达,脱落酸（ABA）、甲基茉莉酸（MJ）和低温处理显著抑制了该基N的表达,伤害处理在一定程度上抑制了该基因的表达。推测AaBBX22基因可能参与青蒿的耐盐和耐旱调控。     

呼伦贝尔草原辉河湿地表层土壤盐分分布特征

土壤盐分是影响湿地水生态质量的重要要素,其分布特征和迁移规律与湿地水分状况息息相关.为探究草原河流湿地不同淹水状态下表层土壤盐渍化特征,2020年7—8月在呼伦贝尔草原辉河湿地,沿辉河干流河道水平和垂直方向分别设置5个取样区和3种淹水状态,采集土壤样品并进行主成分分析.结果表明:辉河湿地盐渍化严重,62.5％的土壤处于...     

不同经营措施对尾巨桉人工林土壤呼吸的影响

以南宁七坡林场不同经营措施下的3年生尾巨桉林为研究对象,于2013年10月至2014年9月,采用Li 8100土壤碳通量系统测定土壤呼吸速率月变化,分析不同经营措施及土壤温度对土壤呼吸的影响。结果显示：尾巨桉林土壤呼吸速率具有明显的季节变化,峰值出现在2013年10月和2014年6月;不同经营措施间的土壤呼吸速率均存在显著差异 （P ＜ 005）,其大小排序为造林密度2m × 3m ＞ 4m × 3m,施肥量08kg/株 ＞ 06kg/株,除杂处理 ＞ 未除杂;2013年10月至2014年9月土壤呼吸与土壤温度存在极显著的指数相关关系 （P ＜ 001）,土壤温度可以解释土壤呼吸速率变化的848%,Q10值为1610。     

辣椒新品种苏椒22号的选育

苏椒22号是以自交系2009X142与2009X200配制而成的早中熟辣椒一代杂种,果实羊角形,平均单果质量28.2 g。果面光滑有光泽,青果浅绿色,成熟果红色。辣味中等,品质好。适宜适宜陕西、湖南、江西、四川、贵州、云南和内蒙古及同类型生态区域露地栽培。     

海南省自沙县示范推广甘蔗脱毒健康种苗新台糖22号探析

针对海南省白沙县甘蔗生产现状,介绍了甘蔗脱毒健康种苗新台糖22号在白沙县试种示范基本情况．分析了白沙县推广应用甘蔗脱毒健康种苗新台糖22号的意义,并从政府重视、加强培训及加大资金投入等方面提出了白沙县推广应用甘蔗脱毒健康种苗新台糖22号的对策     

Coxiella burnetii associated reproductive disorders in domestic animals-a critical review

The bacterium Coxiella burnetii has been detected in the fetal membranes, birth fluids and vaginal mucus, as well as in the milk and other excretions of several domestic mammals. The finding of C. burnetii in association with abortion, parturition and in the postpartum period has led to the hypothesis that C. burnetii causes a range of reproductive diseases. This review critically evaluates the scientific basis for this hypothesis in domestic mammals.The review demonstrates a solid evidence for the association between C. burnetii infection and sporadic cases of abortion, premature delivery, stillbirth and weak offspring in cattle, sheep and goats. C. burnetii induced in-herd epidemics of this complete expression of reproductive failure have been reported for sheep and goats, but not for cattle. The single entities occur only as part of the complex and not as single events such as generally increased stillbirth rate. Studies show that C. burnetii initially infects the placenta and that subsequent spread to the fetus may occur either haematogenous or by the amniotic-oral route. The consequences for the equine, porcine, canine and feline conceptus remains to the elucidated but that infection of the conceptus may occur is documented for most species. There is no solid evidence to support a hypothesis of C. burnetii causing disorders such as subfertility, endometritis/metritis, or retained fetal membranes in any kind of domestic animal species.There is a strong need to validate non-pathology based methods such as polymerase chain reaction for their use in diagnostic and research in relation to establishing C. burnetii as the cause of abortion and to adapt an appropriate study design and include adequate control animals when linking epidemiological findings to C. burnetii or when evaluating effects of vaccination in production herds.     

Seroprevalence of Coxiella burnetii in Australian dogs

The role of dogs in the transmission of Coxiella burnetii to humans is uncertain, and extensive seroprevalence studies of dogs have not been previously conducted in Australia. This study determined C. burnetii exposure in four diverse canine subpopulations by adapting, verifying and comparing an indirect immunofluoresence assay (IFA) and an enzyme‐linked immunosorbent assay (ELISA) used to detect anti‐C. burnetii antibodies in humans. Canine serum samples (n = 1223) were tested with IFA from four subpopulations [breeding establishments; household pets; free‐roaming dogs in Aboriginal communities; shelter dogs]. The proportions of seropositive dogs were as follows: breeding (7/309, 2.3%), household pets (10/328, 3%), Aboriginal communities (21/321, 6.5%) and shelters (5/265, 1.9%). Dogs from Aboriginal communities were 2.8 times (CI 1.5–5.1; P < 0.001) more likely to be seropositive than dogs from other populations. The ELISA was used on 86 of 1223 sera tested with IFA, and a Cohen's Kappa coefficient of 0.60 (CI 0.43–0.78) indicated good agreement between the two assays. This study has established that Australian dogs within all four subpopulations have been exposed to C. burnetii and that a higher seroprevalence was observed amongst free‐roaming dogs associated with Aboriginal communities. As C. burnetii recrudesces during pregnancy and birth products contain the highest concentration of organism, individuals assisting at the time of parturition, those handling pups shortly after birth as well as those residing in the vicinity of whelping dogs are potentially at risk of developing Q fever. However, the identification of active antigen shed in excreta from seropositive dogs is required in order to accurately define and quantify the public health risk.     

Seroprevalence and Factors Associated with Coxiella burnetii Infection in Small Ruminants in Baringo County,Kenya

To improve estimates of C. burnetii epidemiology in Kenya, a survey was undertaken in small ruminants in Baringo County, where acute cases of Q fever in humans had been reported in 2014. From 140 household herds selected, 508 (60.5%) goats and 332 (39.5%) sheep were included and an indirect ELISA assay for C. burnetii IgG antibodies performed. In addition, epidemiological information at both herd and animal level was collected. Generalized mixed‐effects multivariable logistic model using herd as the random effect was used to determine variables correlated to the outcome. Overall seroprevalence was 20.5% (95% CI: 17.8%, 23.3%). Goats had 26.0% (95% CI: 22.2%, 30.0%) compared to sheep 12.2% (95% CI: 8.7%, 16.0%). Nomadic pastoralism, goats and older animals (>1 year) were associated with greater risk of C. burnetii seropositivity (P = ≤0.05). Heterogeneity in C. burnetii seropositivity was observed across the sublocations (P = 0.028). Evidence of C. burnetii exposure in small ruminants revealed poses a potential risk of exposure to the people living in close proximity to the animals. We recommended integrated animal–human surveillance and socio‐economic studies for C. burnetii, to aid our understanding of the risk of transmission between the animals and humans, and in the design of prevention and control strategies for the disease in the region.     

中国四川芭蕉属(芭蕉科)一新变种——雷波野芭蕉

描述了芭蕉属(芭蕉科)一新变种——雷波野芭蕉(Musa luteola J.L.Liu var.leiboensis J.L.Liu et Q.Luo),并绘制了形态图。新变种以其叶基部渐狭至楔形,叶柄和叶鞘两侧具纸质或干膜质的翅,花序下垂,椭圆形或卵状椭圆形,先端圆,苞片外面淡黄色或绿黄色,先端圆或钝圆,每苞片内有花二列,果序球状、近球状或椭圆状球形,果实密集与黄色野芭蕉(Musa luteola J.L.Liu)相近似,但主要又以子房具5～9钝状棱角,浆果较大,长(6.7～)8.5～11.9 cm,宽5～7.2 cm,具5～9钝状棱角,果梗较长1～1.5 cm而有显著区别。