多效唑对柠檬营养生长和生殖生长的影响

[目的]研究多效唑（PP333）对柠檬营养生长和生殖生长的影响,为柠檬生产上控梢促花、提高产量提供参考依据.[方法]柠檬树喷施400、600、800、1000、1200 mg/kg多效唑后,定期统计新梢数量、平均单枝花蕾数量,测定各处理果实纵横径、物理性状、产量等.[结果]喷施不同浓度PP333对柠檬树新梢萌发均有抑制作用,与对照（CK）相比,抑梢效果显著;喷施PP333后植株翌年平均单枝花蕾数量、开花量、着果量均比CK有不同程度增加,并能显著延长柠檬开花时间.平均单枝花蕾数、开花量最多的为800 mg/kg处理,平均单枝最大花蕾量比CK增加53.34％,平均单枝开花量是CK的1.83倍.在生长缓慢期,800、600 mg/kg处理分别对促进柠檬春花果和秋花果果实纵径增加效果明显,而在整个生长发育过程中,PP333对柠檬果实横径和果实物理性状的影响较小;PP333能显著提高柠檬单株产量和公顷产量,其中以800 mg/kg处理产量提高最明显,单株产量和公顷产量分别比CK增加41.07％和41.63％.[结论]适宜浓度的PP333可抑制柠檬树营养生长,促进生殖生长,以800 mg/kg处理效果最佳.     

比久和多效唑混合物的高效液相色谱分析

在吸光度为210nm、流动相配比为甲醇：乙腈：水＝40：20：40的条件下，多效唑和比久均能得到很好的分离。比久的保留时间约为3．19min，多效唑的保留时间约为11．83min。加标回收率：比久为108．69％，多效唑为90．39％。     

Effect of dietary graded levels of cottonseed meal and gossypol on growth performance,body composition and health aspects of allogynogenetic silver crucian carp,Carassius auratus gibelio♀ × Cyprinus carpio♂

A 6‐month trial was conducted to evaluate the effects of dietary cottonseed meal (CSM) and free gossypol (FG) on allogynogenetic silver crucian carp, Carassius auratus gibelio♀ × Cyprinus carpio♂ with 4 replicates of each treatment. Isonitrogenous and isocaloric diets were formulated with the 0 g kg^?1 (control), 200 g kg^?1, 400 g kg^?1, and 560 g kg^?1 CSM. Diets with FG were made by supplementing batches of control diet with 214 mg kg^?1, 428 mg kg^?1, and 642 mg kg^?1. Weight gain, specific growth rate, and protein efficiency ratio increased significantly up to an inclusion level of CSM of 400 g kg^?1 in the diet, with a significant decrease in food conversion ratio. Further increase in CSM to 560 g kg^?1 did not cause further changes in fish performance. Free gossypol did not affect fish performance significantly at any inclusion level. Neither CSM nor FG caused significant effects in any of the other evaluated parameters such as whole body composition, haemoglobin concentration, activities of serum lysozyme, superoxide dismutase, alanine aminotransferase and aspartate aminotransferase, and histology of hepatic tissues and midgut. Our results suggested that crucian carp can tolerate at least 642 mg kg^?1 FG and that it is safe to including 400 g kg^?1 CSM in crucian carp feed.     

调节栽培技术条件下土施多效唑对四季蜜杧反季节开花结果的综合效应

在春季施肥、修剪的情况下,在2005年6月11日,对四年生四季蜜杧单株分别用1.0、2.0、3.0、4.0 g多效唑进行土施处理,结果表明,3.0 g/株处理和4.0 g/株处理的四季蜜杧8月份成花率显著高于对照的,3.0 g/株处理的成花率最高,为87.0%。处理剂量达到或超过3.0 g/株时,叶片变短、变窄,叶片数增加,但枝条长度未受影响。单花序花期9～10 d,开花前期两性花比例高,后期低,2.0 g/株处理的两性花比例最高。从多效唑对四季蜜杧成花率、坐果率、产量以及叶片正常发育等方面的综合影响来看,以2.0 g/株处理的效果较好。     

沙棘果实生长期间维生素含量变化研究

本文对乌鞘岭地区天然分布的中国沙棘和西藏沙棘果实生长与成熟期间维生素C,PP,E等含量的动态变化进行了系统的调查和分析测定。结果表明:V_C主要存在于果肉中,且随果实发育而增加,果实变色期(花后107—110天)达到最高值,随后急速下降。但完熟后果肉中V_C减少时种子内V_C却有所增加。中国沙棘V_C水平远较西藏沙棘为高(5倍以上)。果实发育前期果肉中V_(PP)含量很高,以后逐渐下降,完熟期中国沙棘果实内V_(PP)再度升高,其变化与呼吸速率的变化表现出相同的消长规律。V_E主要存在于种子里,总趋势是随成熟而增加。种子变色期V_E上升至第一高峰值,随后下降,果实完熟后达到第二高峰值。西藏沙棘V_E含量高于中国沙棘。本文对不同维生素可能起的生理作用也进行了讨论。     

PP333对中晚熟水蜜桃品种生长结果影响的研究

对3个中晚熟品种进行不同浓度PP333处理,每品种设每株叶面喷施PP3338000mg/L、10000mg/L、12000mg/L和喷清水对照4个处理,单株为一小区,重复3次,随机区组设计,处理1分2次喷,第一次在5月9日,第二次在6月9日,其余在5月9日喷1次。研究表明,PP333能极显著地抑制枝梢生长。从控制枝梢生长、产量提高、果实形状及圆整度、花芽形成等综合性状分析,4年生大团蜜露以每株喷PP33312000mg/L、5年生湖景蜜露和6年生西浦1号以每株PP3338000mg/L喷2次的处理效果最好。     

Properties of PEG/thermally modified wood flour/polypropylene (PP) composites

In order to improve the dimensional stability of wood-polymer composites, wood flour pre-treated by polyethylene glyco1 (PEG) at two different concentrations and then thermally treated at 140°C, was used as raw material to produce wood flour/polypropylene (PP) composites at a wood content of 40%. The structure of modified wood flour was analyzed with a scanning electron microscope (SEM) and its effect on the physical and mechanical properties of wood flour/PP composites was evaluated. The SEM results indicated the "bulking" effect of PEG on wood flour, which resulted in reduced water uptake. The combination of PEG and heat treatment further improved the moisture resistance of the composites. However, PEG modification had a negative effect on the flexural modulus of rupture (MOR) and the modulus of elasticity (MOE); whereas heat treatment partly compensated for this reduction. For dynamic mechanical properties, PEG treatment decreased the storage modulus (E′). However, the heat treatment resulted in an increase of E′ of the wood flour/PP composites, with the temperature of loss factor peaks shifting to a higher temperature.     

Calculation of E-Moduli of Beams under Third- or Centre-Point Loading: Program for HP-25 Calculator

A method for precision sowing Eucalyptus seeds was needed at the Florida Division of Forestry's nursery. A way to do this is to coat cleaned seeds to form uniform pellets, which are easy to handle with mechanical equipment. The small volume of the seed lots to be pelleted in Florida restricted choice of commercial companies that could do the pelleting. Small volumes (50 to 125 ml) of Eucalyptus robusta Sm. seeds were successfully pelleted in-house with a coating of fine silica sand filler and two polyvinyl alcohol (P.V.A.) binders, and by two commercial companies—Germain's, Inc. and Hilleshog Seed Co., Ltd. Nursery performance of sand-P.V.A. and Hilleshog pellets was similar to that of uncoated seeds, although germination speed was slowed. The binder Gelvatol®,¹ a coldwater soluble P.V.A., had advantages over Elvanol®, a cold-water insoluble binder. Plant yield of Germain's Filcoat® pellets was appreciably less than that of uncoated seeds, but the toughness of the Filcoat® pellets might be an advantage in some sowing situations. The Florida Division of Forestry routinely pellets seeds of several Eucalyptus species in a reciprocating-rotating pan using fine sand and Gelvatol®.     

PP1γ2对树鼩精子成熟和运动性的调控作用研究

为了阐明树鼩精子中是否存在丝氨酸/苏氨酸蛋白磷酸酶1γ2(PP1γ2)及其在附睾精子中的存在形式,进而探究PP1γ2对精子成熟和运动性的调控作用,本试验以树鼩为研究对象,采用Western blotting分析了不同条件下树鼩附睾头和附睾尾精子中PP1γ2的存在形式和磷酸化程度,探讨了双丁酰环腺苷酸(db-cAMP)、3-异丁基-1-甲基黄嘌呤(IBMX)或Ca²⁺对树鼩精子中PP1γ2磷酸化表达水平的影响,并进一步研究了磷酸酶抑制剂冈田酸(okadaic acid,OA)和花萼海绵诱癌素A(calyculin A,CA)对树鼩精子中PP1γ2磷酸化程度的影响及其对树鼩附睾头和附睾尾精子运动度的影响。结果显示,在树鼩附睾头和附睾尾精子中均存在PP1γ2,且在等量的附睾头和附睾尾精子蛋白中,PP1γ2在附睾尾精子的磷酸化程度远高于附睾头精子;db-cAMP、IBMX或Ca²⁺不改变PP1γ2的磷酸化水平;磷酸酶抑制剂OA和CA能明显提高附睾头和附睾尾中PP1γ2磷酸化的程度,且能显著提高精子(尤其是附睾头精子)的运动度(P<0.05),OA和CA的最佳作用浓度分别为1μmol/L和10 nmol/L,最佳作用时间分别为15、20 min。本研究结果表明,蛋白磷酸酶PP1γ2对树鼩精子成熟及运动性具有重要的调控作用,其主要通过磷酸化和去磷酸化的变化发挥作用。     

Low levels of Aflatoxin B1 could cause mortalities in juvenile hybrid sturgeon,Acipenser ruthenus ♂×A. baeri♀

Toxicity of aflatoxin B₁ (AFB₁) was investigated in juvenile hybrid sturgeon Acipenser ruthenus ♂ × A. baeri♀, an important coldwater finfish farmed in China and other countries. Seven experimental diets (Diet A–G) containing different levels of AFB₁ (0, 1, 5, 10, 20, 40 and 80 μg kg^?1 diet) were fed to juvenile sturgeon weighing 10.53 ± 0.17 g kg^?1 to determine its effect on survival, growth, feed consumption, hematocrit, liver histology as well as muscular and hepatic toxin accumulation. The experiment lasted for 35 days and was conducted in two periods of 25 and 10 days each. No external changes or unusual behaviour was observed in the fish fed diets with AFB₁. Mortality was observed in fish fed with highest levels of AFB₁ (80 μg kg^?1– Diet G) from day 12 onwards. After 25 days, fish fed the diet of 80 μg AFB₁ kg^?1 showed significant lower survival (50 ± 5.77%) followed by those fed 40 μg AFB₁ kg^?1 diet (80 ± 5.77%) and 20 μg AFB₁ kg^?1 diet (86.66 ± 3.33%). No significant difference was observed in specific growth rate (SGR) or hepatosomatic index (HSI) between groups. Hematocrit was significantly higher in the fish fed the diet of highest AFB₁. The fish were weighed at day 25 in some treatments (Diets F and G) because of high mortality. However, feeding was continued for another 10 days to observe mortality or behavioural changes if any in the other groups. After 35 days, survival in the fish fed Diet F (40 μg AFB₁ kg^?1) was 40% and those fed Diet E (20 μg AFB₁ kg^?1) was 36.2%. Significant histopathological changes including nuclear hypertrophy, hyperchromasia, extensive biliary hyperplasia, focal hepatocyte necrosis and presence of inflammatory cells were observed in the liver of fish fed high levels of aflatoxin (40 and 80 μg kg^?1). AFB₁ accumulation in fish muscle and liver increased with increased dietary AFB₁ levels. It could be confirmed that 10 μg AFB₁ kg^?1 diet was the maximum allowable level in hybrid sturgeon diet.