Genetic variation in radish (Raphanus sativus L.) germplasm from Pakistan using morphological traits and RAPDs

Genetic diversity of 30 radish (Raphanus sativus L.) accessions was investigated at the phenotypic level with morphological characters and at the DNA level using the random amplified polymorphic DNA (RAPD) technique. Thirty-six morpho-physiological traits were recorded from seedling stage to harvest. The 31 primers used generated 202 RAPD bands, of which 158 (78.2%) were polymorphic. Multivariate procedures were used to classify the germplasm on the basis of phenotypic traits and RAPD fragments. Dendrograms were generated for the Euclidean distance from the morphological data and the Nei's genetic distance from the RAPD markers. Phenotypically, all the accessions were classified into four major groups corresponding to the different forms of cultivated radish. The morphological diversity existing within each of these groups suggested that they should be discriminated into the three botanical convarieties, sativusT (large-rooted), caudatus (pod-type) and oleifer (oilseed-type). Clustering of the accessions did not show any pattern of association between the morphological characters and the collection sites. Instead, landrace groups were associated with their morphological similarities and horticultural uses. On the other hand, the intra-specific genetic relationships of several accessions based on RAPD analysis were related primarily to their collection sites rather than to their phenotypic affinities. The level of polymorphism exhibited by the various convarieties could be exploited in genetic mapping populations to tag economically important traits. These genotypes also could serve as a useful germplasm source for root, leaf, pod and seed. This preliminary study of traditional radish landraces from Pakistan provides useful information regarding their horticultural potential.     

芜菁花叶病毒不同分离物3'-cDNA片段的克隆及序列分析

 从河北、北京、山东泰安和潍坊等地区的萝卜、大白菜、甘蓝、油菜上得到8个芜菁花叶病毒(Turnip mosaic virus,TuMV)分离物,测定了它们3'-末端cDNA片段的序列。根据衣壳蛋白基因(cp)核苷酸序列可以将这8个分离物与另外2个TuMV分离物分为3组:泰安旧镇大白菜(JZBC)、甘蓝(JZGL)和萝卜(JZLB)分离物为一组;北京(BJILB)、潍坊萝卜分离物(WFLB)与引起萝卜红心病的TuMV分离物(WFLB99)为一组;泰安范镇、河北、潍坊(WFLB04)萝卜和泰安旧镇油菜(JZYC)上的TuMV分离物为一组。农壳蛋白氨基酸序列比较结果与此基本一致,所不同的是分离物WFLB99与所有分离物的差异均较大,形成单独一个分支。有必要对TuMV的变异情况和致病机理进行深入研究。     

日光温室萝卜棵间土壤蒸发规律试验

土壤蒸发在农田水量平衡和能量平衡计算中占有重要地位。该文采用微型蒸渗仪测定温室萝卜的棵间土壤蒸发,对萝卜棵间土壤蒸发的变化规律及其与太阳辐射、气温和相对湿度等主要气象因子的关系进行了试验研究和分析。研究结果表明：温室萝卜棵间土壤蒸发随着萝卜生育期的推移有减小的趋势,棵间土壤蒸发量占全生育期总耗水量的37.73%～41.71%,棵间土壤蒸发与太阳辐射、气温和相对湿度等主要气象因子均呈现良好的指数关系,该研究对合理制定温室萝卜的灌溉制度具有重要的参考价值。     

采前喷钙和套袋对华盛顿脐橙果皮病变的影响

以华盛顿脐橙为试验材料,研究了采前喷钙和套袋对果皮病变的影响。结果表明,华盛顿脐橙果皮油胞凹陷和油胞破裂等田间果皮病变高达30%以上;果皮病变与果园管理水平、植株和果皮钙含量密切相关,采前喷钙和套袋处理提高了果实硬度,增加果皮钙含量,降低采后果皮病变率40%～50%,达显著和极显著水平;同时钙处理促进果实着色,显著增加果皮红色度和光泽度。采前喷钙和套袋对提高华脐果实的外观品质和商品性具有很好的作用。     

灭幼脲和氟幼灵防治榆紫叶甲的研究

榆紫叶甲是我国榆树的毁灭性害虫。该虫分布广，密度大，食性单一，常年成灾，给防治带来困难。为了避免常规杀虫剂造成的“１年控制，２年发生，３年成灾”的被动局面，利用昆虫生长调节剂灭幼脲Ⅲ号胶悬剂及油剂和氟幼灵胶悬剂及油剂进行防治，可同时杀死幼虫，成虫和卵。灭幼脲Ⅲ号油剂和氟幼灵油剂可杀卵８５％－９０％左右。两种药剂的胶悬剂和油剂均可杀死幼虫９５％－８４％－９０％，卵的孵化率降为１０．６４％－１９．５７     

施用尿素对冬萝卜产量形成及叶绿素含量的影响

[目的]研究冬萝卜莲座期叶绿素含量及收获期产量与对应施氮肥量的关系。[方法]以广东培育的晚熟冬萝卜为试材,施尿素处理设0、70、130、190、250kg/hm2（分别标记为N0、N70、N130、N190、N250）5个施N水平,动态分析了莲座期冬萝卜叶片叶绿素含量及收获时冬萝卜产量。[结果]5个不同施N水平处理中,冬萝卜叶片叶绿素含量随施N肥量的增加呈对数递增,以N130处理的叶绿素含量最高（0.461mg/kgFW）,且与N190和N250处理间差异不显著;收获时,块根产量以N130处理最高（65.5t/hm2）,N250处理次之（63.5t/hm2）;冬萝卜叶产量以N250处理最高（17.7t/hm2）,N130处理次之（12.6t/hm2）。块根产量和叶产量分别与叶绿素含量间呈极显著正相关（R2=0.9809）和显著正相关（R2=0.7891）。[结论]研究认为,该地区冬萝卜施N肥量应在150kg/hm2为宜。     

Nucleus substitution of Brassica japonica Sieb. with Raphanus sativus L. and its resultant chlorophyll deficiency

Summary Nucleus substitution of Brassica japonica (2n=20) with Raphanus sativus (2n=18) was carried out by means of repeated backcrossing of Brassicoraphavus (2n=37) to R. sativus as a pollen donor. In the course of nucleus substitution, chlorophyll deficiency appeared. Plants with more than 28 chromosomes, like their parents, had green leaves and those with 24 to 26 chromosomes had yellowish green ones. Almost all plants with 18 to 23 chromosomes showed yellow or whitish yellow. The R. sativus with B. japonica cytoplasm (2n=18) was obtained after four successive backerosses. The completely substituted R. sativus showed the same fertility as the true R. sativus used as a recurrent parent. It is assumed that the chlorophyll deficiency is caused by disharmony between the B. japonica cytoplasm and the R. sativus nucleus. The chlorophyll deficiency is discussed in comparison with male sterility or other characters which sometimes occur in alloplasmic Raphanus and Brassica species.     

栽培措施对萝卜生长特性及产量的影响

研究不同栽培措施对萝卜生产的影响,结果表明:试验条件下,播种太早或太迟,对萝卜生长都不利,瑞雪萝卜最适播种期应为9月25日至11月5日;利用地膜覆盖,萝卜地上部和地下部的生产速度明显提高,生育期提早,产量比露地提高了36.64%;每667m2种植密度以6 500~7 500株为好。     

春白萝卜新品种雪单3 号的选育

雪单3 号是利用双单倍体育种技术选育的春白萝卜新品种。母本ED0108A 是以梅花春萝卜× 白玉春萝卜进行花药培
养获得的DH 系为轮回父本，与不育系进行多次回交，转育成的雄性不育系。父本ED3128 是由迟花春萝卜× 天鸿春萝卜进行
花药培养获得的DH 系。该品种生育期60 d（天），植株开展度45 cm，裂叶，小叶11 对，叶片数20，叶簇平展，叶色深绿；
肉质根长圆柱形，白皮白肉，根长36 cm，横径7.1 cm，单根质量1.1 kg，高抗黑腐病、芜菁花叶病毒病、黄瓜花叶病毒病和花
椰菜花叶病毒病，抗霜霉病，群体整齐度高。每667 m² 产量4 000～5 000 kg。适宜在湖北、湖南、河南、山东等地区早春栽培。     

表面油流法显示螺旋离心泵内部流动的研究

对螺旋离心泵叶轮、进水节和进水节与蜗壳交界处的部分端面做了表面油膜、油点试验。通过流谱分析，揭示了表面奇点、分离线与再附着线等流动特征，研究了螺旋离心泵内部流动规律，进而提出了改进螺旋离心泵结构的设想。