JEV、PPV、PRRSV、PRV多联PCR的应用研究

用 JEV、PRRSV二联 PCR,PPV、PRV二联 PCR以及这 4种病毒 4联 PCR对来自内蒙、广州、广西、天津、北京、吉林病料进行检测 ,共检了 1 4 6份病料。其中 PRRSV阳性 7份 ,PPV阳性 1 2份 ,PRV阳性 2 1份 ,PRV和 PPV混合感染 4份。并对 5份人工接种 JEV小鼠病料检测 ,其中 4份为阳性。随后对部分阳性 PCR扩增产物进行点杂交和核苷酸测序鉴定 ,证实了 PCR扩增准确性。对内蒙 PRRSV阳性扩增带测序结果显示 ,我国流行 PRRSV为美洲型 ,在扩增片段的核苷酸序列上有 3个碱基差异     

参与式方法在退耕还林中的应用

本文分析了退耕还林程序和山西省PAAF项目的经验.提出在制订退耕还林县级年度方案、具体实施过程和检查验收等阶段应用半结构访谈、村民大会、实施管理条例以及实施小组和监测小组等参与式工具.     

鸡马立克氏病琼扩抗原的研制及应用

将马立克氏病死鸡的皮肤或羽囊捣碎，制成MD琼扩抗原。试验结果表明，自制MD皮肤和羽囊琼扩抗原与标准抗的检测MD抗体的敏感性和特异性相同，但皮肤琼扩抗原制造方法相对比较简单，产量也较高。     

提高农用潜水电泵修复可靠性的方法

对以QY系列为主体的农用潜水电泵修复可靠性低、寿命短的问题，提出了改进措施，从而有效提高潜水电泵修复可靠性，并延长使用寿命。     

基于工作流的ERP系统重构的研究

分析了企业现有ERP系统运营存在的缺陷，提出了适合本企业的基于工作流的ERP系统重构的开发方案。     

标准差在单粒播种机试验中的应用

单粒播种机试验方法中的标准差与众不同,有很多专业特点,研究它利于提高试验水平,进而利于产品质量的提高。为此,探讨了标准差在单粒播种机试验中的应用。     

Differences in Wheat Cultivar Response to N Supply. I: Differences in Grain Yield Formation

Two-year field trials with winter wheat cultivars Batis and Toronto were conducted in Southern Bavaria, Germany, to investigate the possible causes of cultivar differences in response to N supply varying in total amount and time of application. Cultivar-related differences in grain yields were observed in treatments with low and medium N supply. High doses of N supply resulted in grain yield adjustment or grain yield advantage for cv. Toronto. The results of this study revealed a consistent, genotypic pattern in response to N fertilization in spite of strong seasonal effects. Systematic modifications in canopy growth rates in response to N supply were of particular relevance and a main factor for differences in tillering intensity resulting in modified stand densities. In the present study, cultivar differences in spike development and interactions with N supply related more to abortion than to initiation processes for number of spikelets and number of flowers per spikelet. High grain density (grains per m²) of cv. Toronto was evident during reproduction stages even under conditions of medium N supply. However, decreased growth rates during the later part of grain filling in combination with low 1000 grain weight, which was barely modified by N fertilization, allowed only partial utilization of this potential. It is assumed that sink limitations were of particular relevance for grain yield development in cv. Toronto, while cv. Batis combined a less intense response to N supply with more stability in the development of grain yield components.     

离子选择电极法测定NBPG与银、镉、汞的配合物组成和稳定常数

本文用离子选择电极法测定了N,N—二甲基膦酸基甘氨酸(NBPG)与银、镉、汞的配合物组成和稳定常数。结果表明,NBPG与Ag~ 、Cd~(2 )、Hg~(2 )均生成组成为1:1的多齿质子型配合物。其lgK_稳值分别为:AgL5.28、CdH_3L3.63、CdH_2L5.16、CdHL7.30、CdL11.56、HgH_3L6.06、HgH_2L7.55、HgHL8.24、HgL12.58。     

重庆地区色叶植物资源及其在园林中的应用

色叶植物是未来园林应用的主要造景植物之一，本文就重庆地区色叶植物资源作了详细调查．按植物色彩分成常色叶类、春色叶类、秋色叶类和两季色类，并对其在园林中的应用途径和前景作了详尽介绍．     

氮肥对辣椒果实中辣椒素含量的影响

以田间试验和室内分析相结合的方法,采用景尖椒3号(高辣度),以3种不同的氮肥施用量处理,就生长期中氮肥对辣椒果实中辣椒素含量的影响进行研究.结果发现,在一定条件下,增加氮肥施用量会导致辣椒果实中辣椒素含量减少;果实中过氧化物酶活力与辣椒素含量呈一定负相关.