鼠源细胞TaqMan实时荧光定量PCR检测方法的建立

为对鼠源细胞进行鉴别检测,以鼠线粒体16S rRNA基因序列为靶位点设计特异性引物及探针,建立实时荧光定量PCR检测方法,并评价该方法的特异性及敏感性。结果显示,所建立的检测方法特异性好,针对鼠源细胞基因组荧光定量PCR扩增曲线良好,其他物种来源细胞基因组及生物制品原辅材料未出现特异性扩增曲线;敏感性高,基因拷贝数检出限度为45.3拷贝。本试验建立的荧光定量PCR检测方法能够有效地对鼠源细胞进行快速检测,为细胞质量控制提供了有效方法。     

影响猪采食行为的肠道微生物种类鉴别

动物主要通过采食来获取能量和所需营养物质,探索肠道菌群对猪采食行为的影响并研究其潜在的作用机制,可以为后续通过调控肠道菌群来改善猪采食提供理论基础。以210头商业杜洛克猪为研究对象,使用自动喂料器记录包括平均日采食时间(ADET)、平均日采食次数(ADEV)、平均日采食量(ADFI)等采食行为指标。140日龄时于肛门处收集粪便样品,通过16S rRNA基因V3-V4区测序获得试验猪肠道微生物结构与组成概况,采用Two-part模型以及共丰度组(CAGs)鉴别与猪采食行为相关的肠道微生物种类。结果显示：1)表型间相关性分析结果表明,ADET分别与ADEV(r=0.41,P<0.05)、RFI(r=0.32,P<0.05)呈显著正相关,ADFI分别与RFI(r=0.56,P<0.05)、平均日增重(ADG)(r=0.63,P<0.05)呈显著正相关,另外,RFI与背膘厚呈显著正相关(r=0.16,P<0.05)。2)CAGs分析中,主要包括瘤胃球菌科(Ruminococcaceae)、拟杆菌目(Bacteroidales)以及颤螺菌属(Oscillospira...     

Balance Order and Interpolatory Property of M-band Multiwavelets

The p order balance and interpolatory property is introduced to M band multiwavelets. Balance conditions for M band multiwavelets are investigated in time domain and frequency domain respectively. The balanced order is equal to its approximation order for M band interpolatory multiwavelet.     

Engineering Calculation Mode Based on Reformative B/S Architecture

Engineering designed mode has entered a new stage because of the development of network. New calculating system based on network appeared. By analyzing the common B/S architecture, this paper brings forward an reformative architecture based on Web services and ASP. NET in order to build the new mode of engineering calculation. It can fully implement the sharing of the resources and provide the engineering designers a wonderful calculating environment, and the working eifficiency of designers can be enhanced.     

基于B/S结构的数字地质图元数据信息系统设计与实现

以国土资源环境与区域经济信息系统信息共享元数据内容标准草案(“NREDIS”)为依据,结合ASP.NET技术,利用C#语言,设计了一个基于B/S(浏览器/服务器)结构的数字地质图元数据信息系统。实现了元数据标准的浏览和元数据的输入、查询及维护4大功能。研究成果为国家空间信息基础设施和数字地球的建设提供了一定的理论基础和技术原型,推动了地理空间信息的网络发布和共享。     

The rehabilitation of an irrigation system along the Yellow River

The People's Victory Irrigation System which diverts water from the Yellow River of China covers a total irrigable area of 59 000 ha. The system encountered some serious problems in the first decade of its operation — salinity and waterlogging of irrigated land, siltation of irrigation and drainage channels, as well as a low efficiency of water use. This paper describes a series of structural and functional measures which have been adopted in the past 24 years for the rehabilitation of the system, including: improvement of the existing drainage system and construction of new drainage system on agricultural land, conjunctive use of surface and groundwater supplies, a comprehensive programme to reduce the levels of siltation as well as the implementation of improved water management practices. A model for the optimal operation of the system by using system analysis theory as an aid in reducing the operation and maintenance (O & M) costs has been developed in recent years. The results are that the soil salinity has been controlled and the agricultural production has increased whilst the efficiency of water use has improved and the siltation levels reduced. Experience gained on this system has been successfully used in developing and managing other irrigation projects along the lower reaches of the Yellow River. The proposed procedure of solving the said optimal model has also embodied some benefits from reducing O & M costs in operation.     

基于BIM技术与B/S架构的大坝安全可视化监测系统

为了实现大坝整体结构、安全监测测点布置及监测成果的可视化展示功能,并且能够及时、准确地掌握大坝运行状态和异常情况,以甘再碾压混凝土重力坝为例,对传统大坝安全监测系统存在的问题进行了深入的分析与解答,并围绕问题提出了可视化的大坝安全监测系统思想,利用Revit、Dynamo、Civil3D等主流BIM软件建立了大坝主体结构及监测点的模型族库,集成了大坝及监测系统的三维可视化BIM模型.借用于BIMFACE平台轻量化技术、PHP脚本语言和MySQL数据库等现代化信息技术,开发出基于B/S架构的甘再大坝安全可视化系统,实现了BIM模型的Web轻量化展示,可通过客户端浏览器在三维模型中直观查看大坝结构、监测仪器信息以及监测点时程曲线变化等内容.通过本系统的应用,大大提高了大坝运行期间安全监测的可视化管理水平,同时对传统的大坝安全监测系统提出了现代化的建立方式与思想.     

干旱区B/S模式水库调度系统设计

利用Visual Studio．NET的优良集成开发环境、采用SQL Server 2000作为B／S计算环境数据库服务器的分布式数据管理系统。针对干旱区水库调度的实际特点，选择了适合当地实际特点的预报、调度模型，考虑到该系统维护、升级的需要，系统采用组件技术，对各子系统进行功能分区，模块化处理。系统具备基本信息接收、存储和查询，洪水预报，水位预报，水库调度，成果管理和数据库管理等主要功能。系统界面简洁、友好，功能适用，方便操作。结合实例展示了系统的在干旱区的应用情况以及在进一步推广应用前景。     

Visits to the U.S. Bureau of Reclamation from South Asia and the Middle East, 1946–1990: An indicator of changing international programs and politics

International study programs have contributed to the diffusion of modern irrigation technologies, approaches, and problems. But the patterns and processes of international travel have received little attention to date. This paper examines foreign visits to the US Bureau of Reclamation (USBR) from South Asia and the Middle East from 1946 to 1990. Using data from the Foreign Activities Branch of the USBR, we compare the participation rates from 16 countries in South Asia and the Middle East. India, Turkey, and Egypt had the highest rates of participation, followed by Pakistan, Iran, and Israel. The frequency of visits is influenced by political, economic, cultural, and institutional factors. But the most important factors appear to be foreign relations and geopolitics. Given the problems faced by national irrigation bureaucracies around the world, there is a need to focus more directly on political factors than in the past.