AGPase反义基因转化番茄研究*

 将含有魔芋AGPase反义基因的质粒pBAGP通过冻融法转化到根癌农杆菌（Agrobacterium tumefaciens）菌株LBA4404中,再采用叶盘法将其转化进番茄（Lycopersicon esculentum Mill.）栽培品种“合作908”中,获得含AGPase基因的番茄抗性植株。最后,经卡那抗性鉴定、NPTⅡ基因和AGPase基因PCR扩增和PCR-Southern杂交检测表明,反义AGPase基因成功整合到番茄基因组,为番茄改良品质育种奠定了材料基础。     

38–48 kDa subunits of buckwheat 13S globulins are controlled by a single locus

The inheritance of buckwheat (Fagopyrum esculentum Moench) seed storage proteins was investigated by control crosses between accessions containing different SDS‐PAGE protein patterns in the range of 38–48 kDa. Analysis of segregation of individual F₁ and F₂ seeds obtained by 17 crosses showed segregation of eight electrophoretic bands, from which 12‐banding variants were inferred. Two variants contained one band, nine contained two bands and one variant contained three bands and behaved as a single co‐dominant unit. The segregation of banding variants fits the expected ratios and thus supports the hypothesis of single Mendelian gene inheritance and that alleles are co‐dominant. The results therefore suggest that the genes controlling globulin subunits are tightly linked and inherited as a single locus. The locus was designated Glob‐1 and the twelve segregated protein variants as alleles a‐l. This is the first buckwheat globulin locus identified with multiple alleles, phenotypically expressed as groups of protein bands and thus applicable as a functional marker in buckwheat genomic studies.     

刺嫩芽、蕨菜加工过程中叶绿素含量变化

采用"分光光度法"分别测定刺嫩芽、蕨菜加工过程中各阶段叶绿素含量的结果表明,蕨菜鲜样、第1次腌制、第2次腌制、成品的叶绿素含量分别是328.41、88.95、90.60、47.73ng/g,刺嫩芽鲜样、第1次腌制、第2次腌制、成品的叶绿素含量分别是482.05、157.96、111.14、62.41ng/g。     

野生早樱群落主要乔木种群种间联结性研究

基于2×2联列表,应用方差比率(VR)、卡方(χ2)检验、联结系数(AC)等指标对野生早樱群落中乔木层10个优势种群的种间联结性进行了研究。结果表明:10个优势乔木种群总体呈负的净关联;x2检验表明仅有4个种对表现为显著正联结,3个种对表现为有一定联结性;VR、χ2检验、AC3个参数计算的结果都较为一致,验证了该群落种对间独立性相对较强、相关性较弱的特点。现阶段,该群落种间联结性较为松散或呈负联结状态,一方面与群落所处次生演替初期有关,另一方面可能与各种群生物学特性有关。研究结果对野生早樱制定合适的保护与利用计划具有一定的科学参考价值。     

山麦冬研究进展

综述了近年来山麦冬属植物中湖北麦冬和短葶山麦冬在生物学特性、栽培管理、组织培养、药物学和药理学方面的研究现状及进展。     

叶籽银杏trnS-trnG序列测定与分析

利用叶绿体基因trnS-trnG基因间隔区的通用引物,扩增并分析了不同地区的14个叶籽银杏和2个银杏的trnS-trnG基因间隔区序列,结果表明,14个叶籽银杏和2个银杏的trnS-trnG序列长度均为1005bp,A T的含量为62.19%～62.39%。用DNASTAR软件对14个叶籽银杏和2个银杏的trnS-trnG基因间隔区序列进行同源性和差异性分析,建立了系统进化树。各样品之间变异小,14个叶籽银杏和2个银杏共有23个可变位点,1个信息位点,这些变异都以碱基的置换发生。除这些变异位点外的核苷酸序列完全相同,说明各样品序列之间具有高度同源性。YZ1变异位点较多,与其它样品的同源性较低。所测序列Blast检验后发现叶籽银杏trnS-trnG序列与银杏的相似度最高,苏铁类次之。     

Simulating multiple pest damage in varying winter wheat production situations

The production situation–injury profile paradigm can be used as a framework to assess the harmfulness of multiple-pest complexes in a changing agriculture. A mechanistic simulation model for wheat, WHEATPEST, was developed to incorporate drivers of (i) variable production situations and (ii) their related injury profiles. The model simulates the harmful effects of pathogens, pests, and weeds in a simple, open, generic manner. Simulation drivers were derived from published reports, in particular through a meta-analysis of highly detailed farmers’ field surveys in the United Kingdom and the Netherlands. Preliminary analysis of the model's performances indicates that WHEATPEST conforms with available published reports in a range of production situations and injury profiles. While improvement on some components of the model are discussed, this work points at the need for the collection of cross-disciplinary, reasonably accurate, and standardised data at a system's level, and at the usefulness of modelling tools for basic research and policy.     

天然长白松生物量的研究

天然长白松仅见于长白山北坡海拔630～1 400 m之间,是我国特有的松科松属植物,因其特殊的分类地位,被列为国家Ⅲ级珍稀濒危树种,而受到广泛关注。本文利用多元分析方法,对第一代天然长白松的生产结构和生物量进行研究。给出长白松生物量估测的回归模型及估测参数,指出生物量在长白松天然林中的分配规律,为经营长白山这一特有的种群提供理论依据。     

南方红豆杉容器苗苗木分级研究

采用逐步聚类分析方法,对苗龄为180d的南方红豆杉容器苗苗木分级标准进行初步探讨,提出以苗高和地径作为苗木分级的质量指标,以欧氏距离法对初始分级结果进行修改,经临界值的确定,得出以下分级标准:Ⅰ级苗:树高≥16.5 cm,地径≥0.18 cm ;Ⅱ级苗:16.5 cm＞树高≥9.8 cm,0.18 cm＞地径≥0.14 cm ;Ⅲ级苗:树高＜9.8 cm,地径＜0.14 cm.     

富源县老厂乡灰金竹种群结构与地上部分生物量初步研究

分析富源县老厂乡灰金竹无性系种群的种群结构和地上部分生物量,结果表明:该地区灰金竹种群年龄结构较为合理,部分竹林在无人管理,经营粗放的条件下,年龄结构呈现老化趋势;灰金竹种群的杆龄结构处于壮中龄期,林分株数和蓄积量在杆龄分布上呈现均衡发展;该地区的灰金竹林种群按直径的分布符合正态分布规律,是竹林较理想的直径结构;灰金竹种群的地上部分各构件的生物量所占比例为杆﹥枝﹥叶.