三疣梭子蟹的两种标记技术

本研究采用注射可视嵌入性荧光、剪附肢两种手段对不同期别的三疣梭子蟹进行标记,以研究标记的适用性及对个体生长的影响。对不同发育阶段三疣梭子蟹进行两个部位荧光注射,统计蜕壳后可识别率。结果显示,Ⅱ、Ⅲ期幼蟹适合腹面区域注射,Ⅳ期以后幼蟹适合游泳足基节和头胸甲背面的薄膜关节注射;标记后,经过两次蜕壳,识别率在80％以上,但经3次蜕壳后,识别率较低;根据标记组、未标记组生长数据,通过单因素方差分析发现,标记对三疣梭子蟹个体生长无显著性影响。Ⅶ期以后的幼蟹,更适合剪附肢法,该法操作简单、识别率高,对个体生长、存活无显著性影响（P〉0．05）。     

中草药制剂对泌乳奶牛机体免疫性能的影响

试验采用大青叶、大黄、党参等19味中草药分别组成内服和子宫灌注制剂治疗奶牛子宫内膜炎，并采集颈静脉血测定淋巴细胞刺激指数（SI）和中性粒细胞吞噬力，以观察中草药制剂对奶牛免疫性能的影响。结果显示，中草药制剂均可提高奶牛免疫力，内服中草药制剂应用效果优于子宫灌注制剂（P〈0．05）；复方制剂优于单味中草药制剂（P〈0．05）；复方中草药内服制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高53．76％和4．58％（P〈0．01）；复方子宫灌注制剂可使淋巴细胞刺激指数（SI）和中性粒细胞吞噬力分别提高39．58％和1．98％（P〈0．01）。     

Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.     

广西农产品物流企业WLC品牌元素的选择研究

针对广西农产品物流企业的特点,从众多影响企业品牌建设与发展的因素中,采用AHP-TOPSIS模型选择确定品牌全生命周期阶段对品牌竞争力影响力度最大的品牌元素,以实现品牌的可持续发展。     

4种不同杀虫剂防治淮山斜纹夜蛾药效试验

通过选用4种不同的杀虫剂防治淮山斜纹夜蛾试验，结果表明：药后10d，10亿PIB／mL银纹夜蛾多角体病毒悬浮剂（奥绿一号）处理的防效达100％，0．15％甲氨阿维·1000万PIB／mL苜银夜核悬浮剂（攻蛾）防效达92．8％，15％杜邦安打悬浮剂防效达91．7％，这3种药剂值得在生产上推广应用。     

金针菇航天种菌丝诱变效应的研究

本实验以2个金针菇航天诱变菌种(航金1号和航金2号)和地面对照菌种(江山白)为材料,进行了拮抗实验、菌丝生长速度实验、抗杂性实验和酯酶同工酶分析实验研究,结果表明,金针菇航天种与对照之间有拮抗现象,但无拮抗线。航天搭载菌株的菌丝生长速率比对照快,其中航金1号生长速度最快。航金1号和航金2号均表现出较强的抗杂性,能抑制并盖过霉菌菌丝生长。通过酯酶同工酶聚丙烯酰胺凝胶电泳分析发现,航金1号和航金2号与对照相比都出现了特异谱带,说明经过航天诱变的菌株发生了一定的突变。     

W/O微乳液体系中SA和MS清除·O_2~-的研究

以水溶液体系作为对照,利用分光光度法研究了W/O型微乳液体系中水杨酸（SA）和水杨酸甲酯（MS）清除超氧阴离子自由基（·O2-）的性能.结果发现,在水浴时间为20min、pH值为8.20和邻苯三酚加入量适宜的情况下,W/O微乳液体系中SA、MS对·O2-的清除率分别可达72.92%、81.47%.微小的pH值变化会引起邻苯三酚自氧化速率的显著变化,电解质溶液对其也有明显的影响,但对SA、MS清除·O2-的能力影响不大.     

Development of a ground truthing method for determination of rangeland biomass using canopy reflectance properties

Veld condition assessment and establishment of grazing capacity norms provide guidelines for the formulation of sustainable practices. However, conventional monitoring methods are becoming inadequate to meet future challenges, where quantification of spatial and temporal variation is required. This study proposes an on-site remote sensing method for monitoring above-ground biomass in rangelands. A preliminary model was formulated, based on simple regression relationships between canopy reflectance properties and aboveground biomass. This model was validated in semi-arid environments (Nama-karoo and Kalahari) within the framework of spatial and temporal experimental trials. Model accuracy was found to be primarily a function of canopy structure and vegetation composition where increases in dwarf shrub presence resulted in greater variations of both NDVI (Normalized Difference Vegetation Index) and LAI (Leaf Area Index) measurements. Temporal variation in model accuracy could also be observed which seemed to be associated with precipitation events. It was concluded that the proposed remote sensing method has potential as a ground truthing technique for determination of rangeland biomass. Indications are that this method is well suited for use in grass dominated veld types. With further refinement, implementation of this technique should also be possible in dwarf shrublands.     

乾宁狼尾草和中序狼尾草的某些生物学特性初探与核型分析

从牧草种质资源鉴定利用角度,对中国产狼尾草属的两个新野生种农艺性状生长、栽植密度以及核型分析等方面,进行了比较研究。结果表明,乾宁狼尾草与中序狼尾草在自然状况下,结实率低,分别仅为41.2％和17.8％,但两者营无性繁殖的地下茎十分发达。生长分析的各项指标上,乾宁狼尾草最大叶面积指数13.1比中序狼尾草3.56大,平均净同化作用率前者小,后者大(36.2和54.1克平方米~(-2)周~(-1)),两者差异均极显著(P=0.01),平均相对生长率分别为0.56和0.52克~(-1)周~(-1),与其它各项均不显著。不同栽植密度,使得草种对光照条件、CO_2流通率以及单位植株营养面积表现出巨大差异,导致形成的生物量也不尽同。乾宁狼尾草与中序狼尾草分别以35cm和25cm行距栽植时,当年生物产量较高;从营养成分上,不单含高量的粗蛋白,营养期与开花期分别达21.45％、16.02％和21.05％、16.88％;赖氨酸含量也表现出同步优势。经染色体数目及核型分析,两种的体细胞染色体数均为36,属四倍体(X=9)。其核型彼此无差异。初步可认定两种,亲缘关系属同源染色体组。