Inv(13)(p12 q14)一家系报道

先证者女汉族24岁,身高1.61米.体重60.1公斤。因连续生两胎畸形儿夭折就诊。第一胎为足月顺产,唇裂、腭裂.双脚各为6趾,50天时因呼吸道感染而死亡。第二胎.足月顺产,体征似第一胎,生下两天死亡。外周血染色体检查,分别计数50个细胞,G 显带分析20个核型均为46,xx.Inv(13)(p12 q14)臂间倒位.患者父亲及其兄长核型与患者相同。其丈夫体征与核型均正常。其姐、妹及母亲因居住内地未查,患者哥及姐所生子女表形均无异常。     

中国人白细胞介素-12 (hIL-12)cDNA克隆及序列分析

根据已发表的IL-12两个亚基P35 cDNA序列（NM-000882）与P40 cDNA序列（NM-002187）设计2对引物。以LPS刺激的人脐血淋巴细胞为材料，采用RT-PCR技术从总RNA扩增hIL-12基因，包括完整的前体蛋白编码序列。所得到的序列与GenBank中发表的一致，但与CLMF和NKSF序列有所差异。P35同CLMF相比，244aa密码子GAC（Asp）→GAT（Asp）、247aa密码子ACG（Thr）→ATG（Met）；与NKSF比较，44aa密码子GTC（Val）→GTG（Val）。P40同NKSF比较239aa密码子AAC（Asn）→AAG（Lys），291aa密码子ACC（Thr）→ACG（Thr）；P40同CLMF相比较，氨基酸与核苷酸序列完全一致。通过与不同物种氨基酸及核苷酸序列比较分析，P40亚基与其它动物的同源性80％以上，P35亚基与其它动物同源性在70％。     

Fas对镉激活PC12细胞线粒体凋亡通路的影响

旨在探究死亡受体Fas在镉致大鼠肾上腺嗜铬细胞瘤细胞(PC12)凋亡中的作用及其对线粒体通路的调控机制,用10 μmol·L-1镉处理Fas基因沉默的PC12细胞株12 h,通过Western blot检测BH3相互作用域死亡激动剂(BID)、半胱氨酸蛋白酶-9(caspase-9)、半胱氨酸蛋白酶-3(caspase...     

我国马铃薯价格波动规律研究--基于X-12和H-P滤波法的实证分析

马铃薯作为重要的农产品之一,其价格波动受多种因素影响。本文采用X-12模型和H-P滤波法,选取2005年1月至2015年12月全国农产品批发市场价格的月度数据,将其波动分解为季节性、周期性、趋势性和不规则性波动4种类型。结果显示,马铃薯生产的季节性是价格季节性波动的主要原因;成本上升及消费量增加推动价格趋势性上涨但总体趋于平缓;马铃薯价格呈明显的周期性波动,平均周期约为47个月;天气变化、市场突发事件等是价格不规则性波动的主要影响因素。对此,需扎实推进马铃薯生产稳定发展、健全全产业链信息分析预警机制、完善马铃薯价格保险制度,并推进马铃薯一二三产业融合发展,确保薯农收益稳定增长和市场供需基本平衡,促进市场平稳运行。     

(111)晶面暴露的NiO纳米催化剂的制备及其CO催化氧化性能

采用溶剂热法,以Ni(NO_3)_3·6H_2O、均苯三甲酸以及N,N-二甲基甲酰胺(DMF)为原料快速合成金属有机骨架材料Ni_3(BTC)_2·12H_2O晶体。以Ni_3(BTC)_2·12H_2O为前体,通过焙烧得到具有(111)面的NiO颗粒用于CO催化氧化反应,并与由硝酸镍热解得到的NiO(R)催化剂进行对比。通过X射线衍射(XRD)、N_2物理吸附-脱附、透射电子显微镜(TEM)以及X射线光电子能谱(XPS)等方法对具有(111)面的NiO颗粒的组成、结构和形貌进行表征。CO催化氧化结果表明,具有(111)面的NiO颗粒的催化活性优于普通NiO催化剂的催化活性。     

柱花草编码CP12蛋白的cDNA克隆及其序列分析

叶绿体蛋白12(chloroplast protein 12,CP12)是一个存在于叶绿体与光合作用相关的蛋白。以亲本柱花草热研2号叶片为材料,根据豌豆CP12及柱花草响应低温的cDNASSH文库中CP12克隆片段设计引物,采用RT-PCR,从柱花草cDNA中克隆获得柱花草热研2号CP12基因全长序列,并在GenBank登陆(登录号:HQ906668),命名为SgCP12。柱花草热研2号CP12基因全长426bp,包含399 bp的ORF。通过生物信息学分析结果表明,CP12的C端具有高度保守的结构域。同源性分析表明,该基因与豌豆的CP12基因氨基酸序列同源性最高。     

Validation of a small-area method for estimating the spatial distribution of the degree of tree damage

This article presents a validation of a method, proposed in Podlaski (2003), for estimating the degree of tree damage. This method belongs to a group of small-area methods. To estimate the degree of tree damage in individual P₃ sub-blocks of the SINUS system of information on the natural environment, a classification based on the degree of defoliation was used. Spatial distribution of the degree of tree damage was estimated through unrestricted simple random sampling. The maximum total estimation error for proportions of fir and beech with a damage degree of zero, and also with second and third degrees jointly, reached a maximum of 35.6% for fir, and 33.8% for beech. P₃ sub-blocks characterised by the highest proportion of the healthiest trees (at least 15% being undamaged trees) occurred in patches in the case of fir, and in larger, compact areas in the case of beech. Because spatial diversification in the health of fir, and to a smaller degree in the case of beech, occurs at small scales, small-area methods for estimating tree condition are necessary. An analogous scheme, combining small-area with large-area methods (utilising sub-blocks of the network of large-area systems in a small-area inventory, and establishing a common database), could be used on a wider scale; for example, by supplementing the program of estimating tree health carried out by the United Nations Economic Commission for Europe (Lorenz et al. 2002) with local inventories.     

桑树杂交组合桂桑优12的育成

应用遗传互补和杂种优势原理 ,育成桑树新品种 (杂交组合 )桂桑优 12。其F1群体整齐、发条数多、枝高节密、叶大叶厚、耐剪伐、再生能力强、发芽较早、收造较晚、产叶量较高、叶质较优、繁育较易 ,适宜常规栽培以及加速生丰产和省力化养蚕栽培。与对照沙 2×伦 10 9相比 ,每公顷桑叶产量增产 11 36 % ,桑叶养蚕万头蚕产茧层量增加 4 4 2 % ,10 0kg桑叶产茧量增产 3 18% ,每公顷桑园产茧量增产 19 6 3%。     

Unsaturated vitamin b(12) binding capacity in human and ruminant blood serum - a comparison of techniques including a new technique by high performance liquid chromatography

A new technique by High Performance Liquid Chromatography (HPLC-gel permeation) shows promise as a tool to separate and quantitate the Unsaturated Vitamin B(12) Binding Capacity (UBSC) of the individual Vitamin B(12) binders in blood serum. This method, although not as rapid as protein-coated charcoal or cellulose separation techniques, is more applicable for use with large numbers of samples than gel filtration. The use of a radioactivity detector to monitor the eluant from the column permitted automation of the method. Comparable results for UBBC and for the UBBC of individual binders were obtained when samples were analyzed by gel filtration and HPLC. The HPLC method proved suitably precise and the recovery of added cyanocobalamin was acceptable. It is proposed that HPLC be the method of choice for measurement of the USBC of binders of Vitamin B(12) in blood serum.     

赛加羚羊的分子生物学鉴别

为了解决以往赛加羚羊(Saiga tatarica)鉴别方法存在的不确定性或分辨率低的问题,在mtDNA的12S rRNA基因易变区设计了2对赛加羚羊特异性引物Saiga A和Saiga B,通过PCR扩增和常规电泳检测,分别得到127 bp和320 bp的片段,进而可通过这两个片段的有无来特异性检出赛加羚羊的DNA。引物Saiga A和Saiga B的最小检出量为1．0 ng DNA,稳定性和特异性好、灵敏度很高,可以应用于赛加羚羊样品的检测。