内蒙古绒山羊Hoxa7基因在毛囊中的表达

利用原位杂交技术检测Hoxa7基因在毛囊不同生长时期的表达模式,结果表明,Hoxa7基因分别在胚胎期的95 d初级毛囊的内根鞘表达、在115 d次级毛囊的内根鞘和绒干表达、在125 d初级毛囊内根鞘和毛干皮质表达、在兴盛期10月的初级毛囊毛干皮质和次级毛囊的绒干中表达。揭示在毛囊发育中,Hoxa7基因对于毛囊细胞的增殖可能起一定的作用。     

几类叶面肥对金华大白桃果实经济性状的影响

[目的]筛选有助于增进金华大白桃品质的叶面肥和生长调节剂。[方法]研究7种叶面肥和生长调节剂对金华大白桃果实主要经济性状以及生长发育的影响。[结果]幼果期适时喷施福施壮和S-诱抗素后,大白桃单果重、可溶性固形物含量等果实主要经济性状显著高于对照,并明显优于其他处理。活清浇施处理明显提高了金华大白桃的品质。[结论]喷施福施壮、S-诱抗素和浇施活清对提高金华大白桃品质作用明显,适合在生产上推广应用。     

金华猪繁殖性状的统计学分析

采用金华猪场1979～1999年金华猪的繁殖性能生产记录,测定了2355头金华母猪(纯繁)和金华猪(K)与约克夏(Y)、长白(L)、杜洛克(D)、汉普夏(H)杂交后代的繁殖性状。综合考虑各性状的各种固定效应,并建立各性状的固定效应模型,对其进行方差分析,计算各性状的最小二乘均数(LSM),对影响较大的固定效应在各观察水平上的LSM进行分析与比较。固定效应分析结果表明:品种、胎次、季节、年份对产活仔数、60日龄头数的影响极显著(P<0.01)。而产仔数受胎次、季节、年份的影响极显著(P<0.01)。金华猪纯繁组以及LK×D、K×L杂交组的产仔数、产活仔数较高,K×L、K×Y杂交组合的60日龄头数较高。而其他外来品种之间杂交组合的繁殖性状显著低(P<0.01)。说明金华猪具有高产的优良特性。金华猪产仔数、产活仔数随胎次逐渐递增,6～7胎达到高峰。金华猪夏季产仔数最高为15.72±1.66,显著高于秋、春、冬(P<0.01)。产活仔数春、夏、秋季无差异(P>0.05),但高于冬季(P<0.05)。1982～1987年间金华猪无论是产仔数还是产活仔数整体都维持较高的水平。     

Immunohistochemical characterization of canine prostatic carcinoma and correlation with castration status and castration time

The purpose of this study was to characterize canine prostate cancer using immunohistochemical staining specific for acinar and urothelial/ductal tissue and correlate these results with the dogs' castration status/castration time. Seventy dogs with prostate cancer were included, 71% were castrated and 29% were intact. Compared with an age‐matched control population, castrated dogs were at increased risk of prostate cancer, odds ratio 3.9. Immunohistochemical staining was performed on 58 cases. Forty‐six of the 58 stained positive for cytokeratin 7 (CK 7) (ductal/urothelial origin) and one of the 58 stained positive for prostate‐specific antigen. Dogs with CK 7‐positive tumours were younger when castrated than dogs with CK 7‐negative tumours, 2 versus 7 years (P = 0.03); dogs castrated at ≤2 years of age were more likely to be CK 7‐positive (P = 0.009). These results show that most canine prostatic carcinomas are of ductal/urothelial, androgen‐independent origin. This is consistent with the epidemiological findings, showing increased risk in castrated dogs. Canine prostate cancer may, therefore, not be a realistic model for the human disease.     

《焊工工艺学》多媒体课件的设计与制作

本文介绍了在现代教育思想和教育理论的指导下,结合教学实践,对中等职业学校技工学校机械类焊接专业的专业课<焊工工艺学)CA1课件的设计和制作进行了分析和研究,利用Macromedia公司的多媒体制作软件Authorware7,02设计和制作<焊工工艺学>多媒体教学课件的过程.     

Activities of biotransformation enzymes in pheasant (Phasianus colchicus) and their modulation by in vivo administration of mebendazole and flubendazole

Basal activities of certain pheasant hepatic and intestinal biotransformation enzymes and modulation of their activities by anthelmintics flubendazole (FLBZ) and mebendazole (MBZ) were investigated in subcellular fractions that were prepared from liver and small intestine of control and FLBZ or MBZ treated birds. Several oxidation, reduction and conjugation enzyme activities were assessed. In the liver, treatment of pheasants by FLBZ or MBZ caused very slight or no changes in monooxygenase activities and conjugation enzymes. More significative changes were detected in small intestine. Metyrapone and daunorubicin reductase activities were increased by both substances in the liver. This is the first evidence that certain benzimidazoles modulate reductases of carbonyl group. With respect to the relatively slight extent of the changes caused by FLBZ or MBZ we can assume that repeated administration of therapeutic doses of both FLBZ and MBZ has probably no serious influence on pheasant biotransformation enzyme system.     

Effect of gonadotropin-releasing hormone analogue on human breast cancer cell line in vitro

AIM: To investigate the effects of gonadotropin-releasing hormone (GnRH) analogue on the growth of breast cancer cell lines MCF-7 and MDA-MB-231 in vitro and to explore the related mechanisms with PI3K/Akt or ERK/MAPK pathways. METHODS: The proliferation of human breast cancer cell lines MCF-7 and MDA-MB-231 treatment with triptorelin was detected by MTT assay and the distribution of the cell cycle was determined by flow cytometry. The phosphorylation of the ERK1/2 and Akt was evaluated by Western blotting. RESULTS: Triptorelin inhibited the proliferation of MCF-7 cells at concentration of 10^-5 mol/L after treated for 192 h or at concentration of 10^-4 mol/L after treated for 168 h and 192 h. Triptorelin inhibited the proliferation of MDA-MB-231 cells at concentration of 10^-4 mol/L after treated for 192 h (P<0.05).Treatment with triptorelin for 192 h at concentration of 10^-4 mol/L had no statistical significance effect on phosphorylation of ERK1/2 and Akt(P>0.05).CONCLUSION: Inhibitory effect of GnRH analogue triptorelin on human breast cancer cells is not just the connection with the down-regulation of pituitary hormone, but also a direct inhibitory effect. The role may not be involved in the activation of ERK/MAPK and PI3K/Akt signaling pathways.     

瓠瓜新品种早杂7号的选育

早杂7号是以J138-1-5-6-1-2为母本,以Z2-3-5-8-1为父本配制而成的早熟瓠瓜一代杂种。田间长势中等,早熟性优;商品瓜长35cm左右,横径6.0cm,单瓜质量0.6kg左右;瓜形匀称,瓜皮翠绿有光泽,品质好,VC含量高,坐果率高。适应性强,特别适合浙江、山东、福建等地保护地早熟栽培,也适合保护地秋延后栽培和露地栽培。     

小麦7D染色体数量性状基因定位和效应估计的研究

综合应用方差分析法、区间定位法和联合定位法将控制抽穗期、有效穗数、小穗数、５０粒重和单穗产量等５个性状的６个数量性状基因座（ＱＴＬ）定位在小麦７Ｄ染色体上。其中控制粒重的ＱＴＬ有２个。这些ＱＴＬ集中分布在着丝粒附近和染色体长臂的末端两个区域。效应估计结果表明多数ＱＴＬ的效应是微效的，控制同一个性状（粒重）的两个ＱＴＬ的效应几乎相等。比较３种ＱＴＬ定位法的分析结果，方差分析简单有效，且当染色体上只有     

Development and characterization of common wheat-Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus

We developed some wheat-Th. intermedium translocation lines,Yw642, Yw443 and Yw243, etc., showing good BYDV resistance from L1by induced homoeologous pairing using CS ph mutant. Characterization ofthese wheat lines was carried out by GISH and RFLP analysis. The resultsof GISH showed that the lines, YWw42, Yw443 and Yw243, etc., arehomozygous wheat-Th. intermedium translocation lines, in which thechromosome segments of Th. intermedium were transferred to thedistal end of a pair of wheat chromosomes. RFLP analysis indicated that thetranslocation chromosome of the wheat lines is T7DS · 7DL-7XL. Thebreakpoint of the translocation is located on the distal end of 7DL, betweenXpsr965 and Xpsr680 about 90–99 cm from the centromere. The BYDVgene is located on the distal end of 7XL around Xpsr680, Xpsr687 andXwg380. The RFLP markers of psr680, psr687 and wg380 werecosegregated with the BYDV resistance respectively and could be used formolecular assisted selection (MAS) in wheat breeding program for BYDVresistance.