普通小麦春化基因VRN1 RNA干扰载体构建及转基因小麦获得

为进一步探究春化基因VRN1在小麦发育进程中的功能,利用荧光定量PCR分析了VRN1基因在不同发育特性小麦品种新春2号、京841中的表达情况。结果表明,随着生育进程的推进,VRN1基因在新春2号叶片和茎尖中表达量均呈上升趋势,VRN1基因在京841叶片中呈波动上升趋势,在茎尖中表达量趋于0;以p FGC5941载体为基础构建含有VRN1反向重复序列的RNA干扰载体,利用农杆菌介导的茎尖转化法转化新春2号获得了再生植株,并通过PCR法检测获得了转基因阳性植株,为从分子水平上实现小麦发育特性遗传改良和创育小麦新种质奠定了基础。     

厚皮甜瓜新品种红宝玉的选育

红宝玉是以10R15 为母本、10R8 为父本选育而成的中熟厚皮甜瓜一代杂种。植株长势稳健，抗病抗逆性强。全生育期95～110 d（天），果实发育期30～35 d（天）。果实短椭圆形，成熟后果面乳白色，单果质量约1.5 kg，果肉橘红色，肉厚3.5～4.0 cm，腔小不发酵，中心可溶性固形物含量17.5%，肉质紧脆，不脱蒂，耐贮耐运。平均每667 m²产量3 600 kg 左右。适宜在山东、河北、陕西、安徽等地春季保护地设施栽培。     

Heme oxygenase-1 alleviates alcoholic liver steatosis: histopathological study

Excessive alcohol consumption is one of the most important causes of hepatic steatosis, which involves oxidative stress. In particular, increased oxidative stress has been strongly linked to stimulation of the expression of heme oxygenase-1 (HO-1). This study aimed to investigate whether HO-1 could alleviates alcoholic steatosis in rats. Male Wistar rats were randomly divided into 4 groups: 1) the control group, 2) the EtOH group, 3) the EtOH + ZnPP-IX group and 4) the EtOH + Hemin group. Liver histopathology was investigated in weeks 1 and 4 after the start of the treatment period. Alcohol treatment significantly increased the hepatic malondialdehyde (MDA) levels, an oxidative stress marker. In addition, it increased the triglyceride, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in both weeks. Gross examination demonstrated a yellowish and slightly enlarged liver in the alcohol-treated rats. Hematoxylin and eosin (H&E) and Oil Red O staining indicated hepatic steatosis, which was characterized by diffuse, extensive fatty accumulation and discrete lipid droplets of variable size in hepatocytes of the alcohol-treated rats. Administration of the HO-1 inducer hemin resulted in upregulation of hepatic HO-1 gene expression, reduced the MDA, triglyceride, ALT and AST levels and alleviated alcoholic hepatic steatosis, whereas administration of the HO-1 inhibitor zinc protoporphyrin IX (ZnPP-IX) resulted in downregulation of hepatic HO-1 gene expression and could not alleviate alcoholic hepatic steatosis either week. In conclusion, HO-1 could alleviate alcoholic hepatic steatosis in male Wistar rats and may be useful in development of a new therapeutic approach.     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

涝胁迫对豫楸1号4种砧木嫁接苗渗透调节物质的影响

通过对涝渍胁迫条件下豫楸1号4种砧木嫁接苗渗透调节物质（Pro、可溶性糖、可溶性蛋白）活性的测定,结果表明：在持续涝渍胁迫下,3种渗透调节物质的变化趋势基本一致,整体变化趋势为先上升后下降,只是峰值出现的时间有差异。     

蜂王浆对尾静脉注射4T1细胞小鼠抗氧化能力的影响

探讨蜂王浆对尾静脉注射4T1细胞小鼠的抗氧化和免疫力的影响.将4T1细胞或其与蜂王浆的混合物通过尾静脉注射入小鼠体内,ELISA法检测蜂王浆对小鼠血清、肝脏和肾脏中与抗氧化能力相关的指标及血清中和免疫力相关细胞因子水平的影响.结果表明,和模型对照组相比,蜂王浆(50、100 mg·mL~(-1))可以显著提高小鼠血清内T-AOC、IFN-α(P0.05)水平,降低IL-4的水平(P0.05);蜂王浆剂量为50 mg·m L~(-1)时,IFN-γ的含量显著增加(P0.05).蜂王浆(100 mg·mL~(-1))显著提高肾脏中T-AOC的水平(P0.05).可见,蜂王浆可以改善尾静脉注射4T1细胞小鼠的抗氧化能力和免疫力.     

不同产量潜力甘薯光合产物的生产、运转与块根产量的关系

选取中国主栽的食用型甘薯品种龙薯9号、红香蕉、泰中6号、苏薯8号、遗字138和北京553进行大田试验,研究不同产量潜力甘薯品种光合产物的生产、运转及其与块根产量的关系。结果表明,高产品种整个生育期干物质积累快,光合产物向块根输送多,同化物在块根中的分配率较高,而且高产品种的标记叶合成的光合产物能够较多地运出。因此,叶片光合作用形成的光合产物能够及时地往外运输,是高产甘薯品种高产的重要保证。高产品种具有较高的经济系数。     

核磁共振法测定甲哌鎓原药的含量

采用定量核磁共振法建立了甲哌鎓原药的定量方法。以重水为溶剂,仲丁基脲为内标物,以化学位移分别在δ3.12的甲哌鎓甲基和δ1.10的仲丁基脲甲基的1H-NMR信号作为定量峰。以甲哌鎓与仲丁基脲定量峰的峰面积比(AS/AI)对甲哌鎓与仲丁基脲的质量比(WS/WI)进行线性回归,线性相关系数为0.999 9;重复测定同一批次原药含量,变异系数为0.28%。采用核磁共振波谱法对甲哌鎓进行定量分析,操作简便,测定速度快,准确度高。