A conceptual model of farmers' decision-making process for nitrogen fertilization and irrigation of durum wheat

With a 2 millions of tons production, France is the second country in the European Union to produce durum wheat. Durum wheat production requires high grain nitrogen concentration. Irrigation and nitrogen fertilization must be managed simultaneously to maximize grain yield and also avoid low protein concentration and environmental impacts. To help advisors and farmers to better manage together these two agricultural operations and to develop innovative managements, developing a biodecisional model is an interesting possibility. However, knowledge is still missing on how farmers already managed these operations and how these two operations are linked. We developed the conceptual model for the decision part of this computer model. We performed a survey of 28 farmers conducted over the five French production areas investigating a diversity of growing conditions to identify the set of possible constraints and farmers' decision rules. To analyze the survey, we first used a general inductive approach on individual cases and then built a conceptual model of the decision with a bottom-up approach. We identified four decision sequences for fertilization (N splitting, choice of N fertilizer, rate of application, fertilization triggering) and five for irrigation (irrigation period, anticipated number of irrigation cycles, irrigation cycles organization, irrigation triggering and irrigation cycle specificities). For each operation, the first three decision sequences refer to strategic decisions. The other decision sequences refer to tactical decisions. Coupling this model with a crop model could provide guidelines for managing durum wheat in the current climatic and economic changing context.     

北川白山羊基因组与表型性状相关的多态性RAPD标记

北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量（P=0.028）、体高（P=0.017）和体长（P=0.037）具有显著影响,SBS02对体质量（P=0.033）和体高（P=0.034）具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。     

Contribution of green manure legumes to nitrogen dynamics in traditional winter wheat cropping system in the Loess Plateau of China

Excessive application of N fertilizer in pursuit of higher yields is common due to poor soil fertility and low crop productivity. However, this practice causes serious soil depletion and N loss in the traditional wheat cropping system in the Loess Plateau of China. Growing summer legumes as the green manure (GM) crop is a viable solution because of its unique ability to fix atmospheric N₂. Actually, little is known about the contribution of GM N to grain and N utilization in the subsequent crop. Therefore, we conducted a four-year field experiment with four winter wheat-based rotations (summer fallow-wheat, Huai bean–wheat, soybean–wheat, and mung bean–wheat) and four nitrogen fertilizer rates applied to wheat (0, 108, 135, and 162 kg N/ha) to investigate the fate of GM nitrogen via decomposition, utilization by wheat, and contribution to grain production and nitrogen economy through GM legumes. Here we showed that GM legumes accumulated 53–76 kg N/ha per year. After decomposing for approximately one year, more than 32 kg N/ha was released from GM legumes. The amount of nitrogen released via GM decomposition that was subsequently utilized by wheat was 7–27 kg N/ha. Incorporation of GM legumes effectively replaced 13–48% (average 31%) of the applied mineral nitrogen fertilizer. Additionally, the GM approach during the fallow period reduced the risk of nitrate-N leaching to depths of 0–100 cm and 100–200 cm by 4.8 and 19.6 kg N/ha, respectively. The soil nitrogen pool was effectively improved by incorporation of GM legumes at the times of wheat sowing. Cultivation of leguminous GM during summer is a better option than bare fallow to maintain the soil nitrogen pool, and decrease the rates required for N fertilization not only in the Loess Plateau of China but also in other similar dryland regions worldwide.     

基于叶气温差的生育中期冬小麦水分亏缺诊断研究

为了获取叶片尺度基于叶气温差的冬小麦生育中期水分亏缺诊断阈值,分析了冬小麦叶气温差的典型日变化及其与土壤水分、空气温度及太阳辐射等因子的相互关系,揭示了冬小麦叶气温差对光合气体交换参数的影响,确定了冬小麦叶片水分利用效率较适宜的非气孔限制值及叶气温差范围。结果表明,冬小麦叶气温差在不同的土壤水分条件下表现出不同的日变化规律,随光量子通量增加而升高,随土壤水分、气温增加而降低;4、5和6月冬小麦叶片水分利用效率较适宜的非气孔限制值范围分别为0.7~1.3、1.1~2.0和0.9~1.5,叶气温差控制范围分别为-1.2~0.4、-1.5~-1和-1.25~-0.9℃。     

人工合成小麦紫色胚芽鞘的遗传定位分析

为探明小麦花青素色素沉积的关键位点,以167份重组自交系群体为材料,利用已构建的高密度遗传图谱对来源于硬粒小麦(AABB)和节节麦亚种tauschii(DD)杂交后所得的人工合成小麦SHW-L1中控制紫色胚芽鞘的基因进行遗传定位。同时利用目标性状关联遗传区段对SHW-L1及其亲本进行基因型鉴定。结果表明:目标性状在重组自交系群体中表现为单基因遗传,且被定位于7D染色体,其关联遗传区段在异源六倍化过程中有遗传位点缺失。     

小麦TaeEF1β基因的克隆、同源性及表达分析

真核翻译延伸因子(eukaryotic translation elongation factor,eEFs)是一种重要的多功能调控蛋白,eEF1β是eEF1的组成部分,在蛋白质生物合成过程中发挥着重要的作用。本文通过RT-PCR扩增克隆小麦(Triticum aestivum L.)的eEF1β基因,并命名为TaeEF1β。氨基酸同源性分析发现,TaeEF1β具有高度保守性,且其保守结构域位于137~226 aa处。qRT-PCR结果表明,中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)侵染小麦植株后,可以诱导TaeEF1β基因转录水平的上调表达。另外,本文也进一步分析了TaeEF1β基因在小麦根、茎、叶的表达水平和CWMV侵染不同时间点的表达情况。     

芮城县小麦扬花后期病虫害防治技术

通过对小麦田间后期观测,针对芮城县小麦栽培区,提出小麦扬花后期病虫害防治技术,进而促进该县小麦高产、高效生产。     

Redox halopriming: A Promising Strategy for Inducing Salt Tolerance in Bread Wheat

New strategies to enhance growth and productivity of food crops in saline soils represent important research priorities. This study has investigated the role of certain priming techniques to induce salt tolerance of bread wheat. Wheat grains were soaked in 0.2 mm sodium nitroprusside as nitric oxide donor (redox priming), diluted sea water (halopriming) and the combination of both (redox halopriming). Grains were also soaked in distilled water (hydropriming); in addition, untreated grains were taken as control. Our results indicated that priming treatments significantly improved all growth traits and increased leaf pigments concentration as compared to the control. Priming treatments markedly enhanced membrane stability index, proline, total soluble sugars and K⁺ concentration with simultaneous decrease in the concentration of Na⁺ and malondialdehyde (MDA). Furthermore, yield and yield‐related traits such as plant height, spike length, total number of tillers, 1000‐grain weight, straw and grain yield considerably affected by priming treatments. Moreover, the grain yield of both genotypes was positively affected by redox halopriming treatment. However, the extent of enhancement was more prominent in Gemmiza‐9 (salt sensitive) than that in Sakha‐93 (salt‐tolerant). Overall, this study clearly indicated that redox halopriming treatment is a promising and handy technique to induce salinity tolerance of wheat genotypes.     

Use of Porcine Meal in Plant‐based Practical Diets for Pacific White Shrimp,Litopenaeus vannamei

Two growth trials were conducted to evaluate and confirm the efficacy of a porcine meal (PM) with high protein content (>90%) as an alternative feed ingredient in commercial‐type feed formulation for Pacific white shrimp, Litopenaeus vannamei. Six experimental diets were formulated for the two growth trials. The first five diets contained increasing levels (0, 1, 2, 4, and 6%) of PM as a replacement for soybean meal in a plant‐based diet with low inclusion level (6%) of fish meal (FM). The last experimental diet was produced utilizing 4.2% PM to completely replace FM. In Trial 1, shrimp (1.5 g initial mean weight, 20 shrimp/tank, n = 4) were offered test diets for 6 wk in a semirecirculation system. At the end of Trial 1, shrimp fed with the diet containing 6% PM exhibited significantly enhanced weight gain (WG), feed conversion ratio (FCR), and survival compared to those fed with the diet devoid of FM. As survival was poor across all treatments and different densities could mask growth results the trial was repeated. In Trial 2, shrimp (0.85 g, 15 shrimp/tank, n = 4) were offered diets for 6 wk. Dietary supplementation of PM at 6% significantly improved WG, FCR, and apparent net protein retention in contrast with the treatment devoid of FM, confirming the same trends in Trial 1. No significant difference was detected in protein, lipid, moisture, and mineral profiles of whole‐body shrimp as well as survival across all the treatments. Results of this study indicate that PM is a good high protein source in shrimp feeds, which can be included up to 6% in the low FM‐based diet without compromising the growth of shrimp.     

Modelling Wheat Stomatal Resistance in Hourly Time Steps from Micrometeorological Variables and Soil Water Status

An accurate estimation of stomatal resistance (r_S) also under drought stress conditions is of pivotal importance for any process‐based prediction of transpiration and the energy budget of real crop canopies and quantification of drought stress. A new model for r_S was developed and parameterized for winter wheat using data from field experiments accounting for the influences of net radiation (R_Net), air temperature (T_Air) and vapour pressure deficit of the atmosphere (VPD) interacting with an average water potential in the rooted soil (ψ_RootedSoil). r_S is simulated with a limiting factor approach as maximum of the metabolic (related to photosynthesis) and hydraulic (related to drought stress) acting influences assuming that, if drought stress occurs, it will dominate stomatal control: r_S = max(r_S(T_Air), r_S(R_Net), r_S(VPD, ψ_RootedSoil)). This transitional approach is suited to reproduce measured daily time courses of r_S with a varying accuracy for the single measurement dates but performed satisfactorily for the whole data set (r² = 0.63, RMSE = 59 s m^?1, EF = 0.60). This new semi‐empiric approach calculates r_S directly from external environmental conditions. Therefore, it can be easily implemented in existing model frameworks as link between operational crop growth models that use the concept of radiation use efficiency instead of mechanistic photosynthesis modelling and soil–vegetation–atmosphere transport models.