外源一氧化氮供体对盐胁迫下小麦幼苗叶片谷胱甘肽抗氧化酶系统的影响

研究了外源一氧化氮（NO）供体SNP对150 mmol/L NaCl胁迫下小麦幼苗叶片谷胱甘肽抗氧化酶系统的影响。结果表明，与单独盐胁迫相比，0.1 mmol/L的SNP处理明显提高了小麦幼苗叶片还原型谷胱甘肽（GSH）的含量，略微降低了氧化型谷胱甘肽（GSSG）的含量，明显提高了GSH/GSSG，这可能与其诱导谷胱甘肽还原酶（GR）的活性有部分关     

真胃变位奶牛抗氧化能力研究

为了阐明奶牛发生真胃变位(abomasal displacement,DA)时机体抗氧化能力的变化规律,本试验采用分光光度法对27头真胃左方变位奶牛与13头健康奶牛血浆总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、总抗氧化能力(T-AOC)、丙二醛(MDA)含量进行了测定.结果 表明,与健康奶牛相比,...     

L-dopa induction of pheochromocytoma 12 cells apoptosis and the protective function of glutathione

AIM:To study the induction of apoptosis of pheochromocytoma (PC12) cell by L - dopa and the clinical significance. METHODS: Using PC12 cells as the medel of dopaminergic neurons,in addition to electron microscopy, agarose gel electrophoresis and flow cytometry, the apoptosis ratio by L - dopa and the effect of antioxi- dant glutathione on PC12 cells were observed. RESULTS: Treabent of PC12 cells with L - dopa in concentrations of 50 μmol/L, 100μmol/L and 150 μmol/L respectively for 24 hours, results revealed that the apoptosis ratio was 12.8%, 24.4%, 37.2%, respectively, which is in cobant with fragment pragment propartions of agarose gel electrophoresis, mere higher than that of control(2.3% ) (P < 0.01 ). The apoptosis ratio of glutathione + L - dopa group was similar to that of control (P > 0.05). CONCLUTION: Induction of apoptosis by L - dopa could be inhibited by glutathione, and oxidative damage may be involved in the pathophysiology of dopaminergic neurons death after long - term treat- ment of L - dopa.     

牦牛谷胱甘肽过氧化酶1基因(GPX1)的克隆及系统发育分析

本研究克隆了牦牛谷胱甘肽过氧化酶1GPX1基因的CDS区序列,分析了其核苷酸序列,并进行了系统发育分析.结果表明,牦牛GPX1基因CDS区全长618 bp,编码205个氨基酸;经与GenBank中其他物种GPX1基因CDS区比对,牦牛GPX1基因CDS区与普通牛和瘤牛完全一致,与水牛、绵羊和猪的序列一致性较高,与其他哺...     

植物抗氧化剂谷胱甘肽研究进展

谷胱甘肽(glutathione,GSH)是植物体内一种重要的抗氧化剂,可以清除细胞代谢过程中产生的多余活性氧自由基,减少由于膜脂过氧化作用而对细胞造成的伤害,在植物抵抗逆境胁迫中起着非常重要的作用.本文主要从植物体内GSH的种类、GSH对植物细胞保护的生理机制(清除细胞内自由基、与有毒重金属物质结合形成无毒化合物、吸收和转运氨基酸)和GSH对逆境胁迫(温度胁迫、干旱胁迫、重金属胁迫和盐胁迫)植物的保护作用等方面进行了总结分析.GSH的研究对于深入了解和认识植物抗氧化剂在代谢过程中的生理作用及清除活性氧自由基的机理具有积极作用,对研究植物抗逆性具有重要的意义.     

Effect of curcuminoids on oxidative stress resistance in Caenorhabditis elegans

AIM：To examine the effect of curcuminoids, including curcumin (Cur), demethoxycurcumin (DMC) and bisdernethoxycurcumin (BDMC), on oxidative stress resistance in Caenorhabditis(C.) elegans. METHODS：C. elegans strain N2 was used in the study and divided into control group and curcuminoids treatment groups. The survival of the worms under juglone-induced oxidative stress was measured. Intracellular reactive oxygen species (ROS) was determined in treated and control C. elegans strain N2 using 2,7-dichlorofluorescein diacetate (H 2DCF-DA). To study the effect of curcuminoids on the expression of glutathione S-transferase 4 (GST-4), the C. elegans strain CL2166 (GST-4::GFP::NLS) was used. The fluorescence images of GFP were captured by fluorescence microscope in treated and control C. elegans and quantified by Image-Pro Plus software. RESULTS：Pretreatment with Cur, DMC or BDMC significantly increased the survival of the worms exposed to juglone-induced oxidative stress. Accumulation of ROS in the worms after oxidative stress was prevented by the treatment with curcuminoids. Moreover, the expression of antioxidative-related protein GST-4 was significantly induced by Cur and DMC. CONCLUSION：Curcuminoids exert significant in vivo antioxidant activity against juglone-induced oxidative stress in C. elegans, which may associate with the attenuating ROS accumulation under the condition of oxidative stress and the increase in the expression of antioxidant-related protein GST-4 in the worms.     

氮素形态对烤烟成熟过程中抗坏血酸-谷胱甘肽循环的影响

探讨了氮素形态对烤烟成熟过程中抗坏血酸-谷胱甘肽循环系统的影响。结果表明,烟叶自移栽后60 d开始,抗坏血酸过氧化物酶(APX)活性和抗坏血酸(ASA)含量逐渐上升,到70~80 d达到最高峰,随后稳定在一定的水平。3种不同氮素形态处理的APX活性和ASA含量变化规律相似,均随着硝态氮比例的增加而增大;谷胱甘肽还原酶(GR)活性在移栽后90 d达最大值,此后逐渐下降,谷胱甘肽(GSH)含量随着烟叶的成熟持续下降。烟叶成熟过程中各处理GR活性和GSH含量随着铵态氮比例的增加而增大。     

柞蚕饰腹寄蝇不同地理种群的杀虫剂靶标酶和解毒酶活性分析

昆虫通过降低靶标酶敏感性及增强对杀虫剂的代谢能力产生抗药性。蝇毒磷是防治柞蚕饰腹寄蝇(Blepharipa tibia-lis Chao)的常用有机磷杀虫剂,为探讨柞蚕饰腹寄蝇不同地理种群对蝇毒磷的抗药性分化程度,分析来自辽宁省瓦房店市、凤城市、庄河市、开原市和西丰县的5个柞蚕饰腹寄蝇地理种群体内的乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GST)、细胞色素P450(CYP450)和羧酸酯酶(CarE)4种酶的活性及蝇毒磷对这些酶活性的体外抑制率。结果表明:来自瓦房店市和凤城市的柞蚕饰腹寄蝇地理种群的AChE比活力及蝇毒磷对酶活性的体外抑制率均显著高于其它3个地理种群;瓦房店市地理种群的GST比活力显著高于其它4个地理种群,蝇毒磷对酶活性的体外抑制率显著高于除庄河市地理种群外的其它3个地理种群;庄河市地理种群的CYP450比活力显著高于来自瓦房店市、凤城市和西丰县的3个地理种群,但与开原市地理种群相比差异不显著,蝇毒磷对瓦房店市地理种群的CYP450活性的体外抑制率显著高于其它4个地理种群;瓦房店市与凤城市地理种群的CarE比活力均显著高于来自庄河市、开原市和西丰县的3个地理种群;蝇毒磷对瓦房店市和凤城市地理种群的CarE活性的体外抑制率显著高于庄河市地理种群,对其它地理种群间的CarE活性的体外抑制率差异不显著。根据以上检测结果,推测来自庄河市的柞蚕饰腹寄蝇地理种群对蝇毒磷的抗性相对较强,而来自瓦房店市的柞蚕饰腹寄蝇地理种群对蝇毒磷的抗性相对较弱。     

A plant-specific tau class glutathione S-transferase from Oryza sativa with very high activity against 1-chloro-2,4-dinitrobenzene and chloroacetanilide herbicides

A plant-specific tau class GST gene homolog was successfully cloned from an Oryza sativa cDNA library by PCR using oligonucleotide primers based on the OsGSTU4 (GenBank Accession No. AF309378) sequence. The cDNA was composed of a 720-bp open reading frame encoding 239 amino acids. The deduced amino acid sequence of this gene shared over 65% sequence identity with the sequences of the tau class TaGST28e45 and ZmGST42. Conversely, the OsGSTU4 sequence showed very low identity to the GST sequences of phi, theta and zeta classes. This gene was expressed in Escherichia coli with the pET vector system, and the gene product was purified to homogeneity using GSH-Sepharose affinity column chromatography. The expressed OsGSTU4 formed a homodimer with subunits of approximately 25.5 kDa. OsGSTU4 displayed very high activity toward 1-chloro-2,4-dinitrobenzene. The activity of the OsGSTU4 was significantly inhibited by S-hexylglutathione and hematin. Plant OsGSTU4 had a unique herbicide specificity and played an important role in the detoxification reaction against fluorodifen and chloroacetanilide herbicides.     

日粮硒对雏鸡血液、淋巴组织含硒量及谷胱苷肽过氧化物酶活力影响的研究

本实验用200只一日龄白洛克肉用雏鸡随机分为三组,分别饲喂低晒基础日粮和添加硒0.2、0.5毫克/公斤的日粮,研究了2—9周龄内雏鸡血液。部分淋巴器官组织含硒量及GSHpx对不同硒水平日粮的反应。结果表明血硒和组织含硒量、组织GSHpx活性间,均有显著的线性相关。胸腺、脾、法氏囊三组织中,脾组织含硒量较高,并具有较高的GSHpx活力。还表明法氏囊在低硒营养时组织含硒量受影响幅度大,而胸腺、脾组织含硒量则相对较稳定。