基因型和环境对面团流变学特性的影响研究

用揉混仪和质构仪研究了3个不同品质类型的冬小麦品种在8个不同的生长环境条件下的面团流变学特性,分析了基因型和环境对性状的影响及各性状间的相关性。结果表明：面团流变学特性受基因型（G）和环境（E）及其互作的共同作用,其中基因型方差均值＞环境方差均值＞G×E互作均值;从性状的表现看,基因型以济麦20表现最佳,环境以烟台点综合表现最好;相关分析表明,面团粘度性状与最大拉伸阻力、拉伸比、峰值时间、峰值面积和8min带宽呈显著负相关,而与延伸度呈显著正相关。最大拉伸阻力与峰值时间、峰值面积和8min带宽呈显著正相关。因此,进行品质评价时,可用质构仪测定的面团粘度特性对面团品质进行初步快速的评定。     

谷蛋白溶涨指数与面团特性和面条品质的关系

 采用谷蛋白溶涨指数 (SIG)法及新的蛋白质组分分离方法 ,分析了 2 5个中国小麦品种的蛋白质组分含量 ,并对面团物理特性、面条制作特性和蒸煮品质进行了评价。结果表明 ,不同溶涨时间 (min)SIG与不溶性谷蛋白含量呈极显著正相关 (r=0 .80 8～ 0 .86 7;P <0 .0 1) ,说明SIG可间接反映不溶性谷蛋白的含量。蛋白质含量、面筋指数、稳定时间、拉伸长度、拉伸能量与SIG5、SIG2 0显著正相关 (r=0 .5 16～ 0 .734;P <0 .0 5 ,0 .0 1) ,说明SI     

Bread-making performances of durum wheat semolina,as affected by ageing

The present study addresses the influence of ageing on the bread-making performances of durum wheat semolina by bread image analysis. Bread loaves were produced from semolina samples stored in 4 different packaging materials for up to 150 days. Sampling and bread-making trials were performed every 15 days. Results showed that ageing does affect durum wheat semolina bread-making performances, highlighting that storage time, rather than the type of packaging material, is the main factor determining quality changes in the final bread samples. In particular, a change in the crust colour parameters and a reduction of the bread slice area and height by 20–35% were observed with increasing semolina storage time, along with a slight increase of crust % area. The change in farinographic parameters of dough suggests that the observed reduction of technological quality might be due to increased stiffness and reduced extensibility of gluten occurring in aged semolina. Unlike common wheat flours, which usually improve their technological features during ageing, durum wheat semolina is negatively affected in its quality by storage. The study highlights the importance of considering bread-making performances among the quality parameters to be taken into account for the shelf life evaluation of durum wheat semolina.     

Sprouting as a pre-processing for producing quinoa-enriched bread

The impact of 48 h sprouted quinoa (SQ) was assessed in bread-making. Wheat flour (WF) was replaced with SQ at different levels (i.e., 10:90, 20:80 and 30:70, SQ:WF ratio). Once the optimal replacement level of SQ was identified, the bread-making performance of this ingredient was compared with those of pearled quinoa (PQ), commonly used in bread-making.Starch pasting properties and gluten aggregation behavior were not strongly affected at 20:80 level. Regardless the replacement level, SQ caused an increase in dough water absorption and in softening degree, and a decrease in stability, suggesting weakening of the gluten network. During leavening, SQ improved dough development and gas production, due to increased sugar content (i.e. maltose, sucrose and D-glucose). The best bread-making performance (highest bread specific volume and lowest crumb firmness) was obtained at 20:80 replacement level. Compared to PQ, SQ exhibited the best leavening capacity (high dough development, gas production and gas retention) and bread properties (high specific volume and low crumb firmness), likely due to its higher sugar content. Moreover, 20SQ bread was characterized by a decreased bitterness assessed by electronic-tongue. In conclusion, sprouting might be considered a valid alternative to pearling to improve the characteristics of quinoa enriched bread     

A novel method to prepare gluten-free dough using a meso-structured whey protein particle system

This paper presents a novel concept for making an elastic dough using a structured protein suspension. The idea behind it is based on the hypothesis that a number of gluten properties originate from a particle structure present in the gluten network. Three different mesoscopically structured whey protein suspensions were produced: whey protein aggregates, a whey protein cold set gel and whey protein particles. Dough mixtures or batters were prepared by mixing the structured protein particle suspension with starch. Farinograph curves, small and large deformation experiments showed that the presence of a mesoscopic protein structure had a large impact on the properties of gluten-free starch mixtures. The whey protein that was structured into a mesoscopic particle suspension changed the starch mixture from a liquid into a cohesive material, having strain hardening properties.     

Alcalase treatment for elimination of stickiness in pikeperch (Sander lucioperca L.) eggs under controlled conditions

Elimination of egg stickiness is an important factor in artificial reproduction of pikeperch (Sander lucioperca L.). This study was conducted to evaluate the effectiveness of Alcalase enzyme to remove the adhesive layer of pikeperch eggs. The eggs were treated with Alcalase at 0.5, 1.0, 1.5, 2.0 and 5.0 mL L^?1 or a milk/talc solution 2 min post insemination. Duration of exposure was 2 min in Alcalase and 60 min in milk/talc. The highest, albeit not significant, hatching rate (85.4%) was found with 1.5 mL L^?1 of Alcalase, but hatching rates were similar in 0.5, 1.0 and 2.0 mL L^?1. Hatching rates were significantly lower groups treated with 5.0 mL L^?1 Alcalase enzyme (56.4%) compared to groups treated with milk powder and talc (61.3%). Nominally complete removal of adhesiveness was observed in 1.5 and 2 mL L^?1. All Alcalase treatments led to significantly lower incubation duration compared with the traditional milk/talc treatment. The application of Alcalase successfully eliminated pikeperch egg stickiness in less time than with traditional milk/clay/talc methods.     

Effects of composition on dough development and air entrainment in doughs made from gluten-starch blends

Gluten and starch are the two main ingredients of a wheat flour dough and it is expected that the extent of air occlusion into the dough would be affected by differences in their relative ratios. The objectives of this paper were to investigate the hydration and development of gluten and how these key events in dough mixing affected air occlusion in gluten-starch doughs. For gluten-starch doughs of the same gluten content, decreasing the water absorption shortened development time and decreased dough density. For formulations of the same water absorption, decreasing the gluten content prolonged the time to development and increased dough density, reflecting less net air entrainment into the dough. The ratios of gluten, starch and water strongly influenced the development of the dough into a good gas-holding material, with the extent of gas entrainment during mixing being evident in measurements of both dough consistency and dough development time.     

Glucose and pyranose oxidase improve bread dough stability

When used in bread dough systems, glucose oxidase (GO) and pyranose oxidase (P₂O) generate H₂O₂ from O₂. We here studied their potential to improve dough and bread characteristics. Neither GO nor P₂O significantly affected the volume of straight dough bread produced with fermentation and proofing times of respectively 90 and 36 min at dosages up to 0.50 nkat/g flour. Supplementation with 1.00 nkat/g flour of GO or P₂O significantly decreased bread loaf volume. The resistance of dough (fermented for 20 min and proofed for 56 min) to an applied shock was substantially improved by inclusion of 0.08, 0.25, 0.50 or 1.00 nkat/g flour of GO or P₂O in the dough recipe. Thus, the proofed doughs showed significantly less collapse and the resultant breads had higher loaf volumes than did the reference breads. Yeast probably exerts an oxidizing effect on dough, which, depending on the exact breadmaking protocol used, might veil the positive oxidizing effect of the enzymes on dough properties during prolonged fermentation.     

Significant down-regulation of γ-gliadins has minor effect on gluten and starch properties of bread wheat

The contribution of gliadins to the baking performance of wheat has been widely discussed. In the present study we report the properties of the flours of fifteen transgenic wheat lines with γ-gliadins down-regulated by RNAi. Technological properties were assessed by sodium dodecyl sulfate sedimentation (SDSS) test, and rheological properties were characterized during mixing using the Mixolab^®. Changes in the proportions of gluten proteins, with significant increases of glutenins, were observed in transgenic lines. These changes did not affect the SDSS test in most of the transgenic lines. The dough strength of some transgenic lines was reduced as determined by the Mixolab^®. Changes in the starch behavior were also observed in the transgenic lines, in which lower values of torque were observed at the C3, C4 and C5 points of the mixing curve. The results reported here are important in understanding the role of γ-gliadins in the bread-making quality of flours.     

Dough rheology and bread quality of wheat-chickpea flour blends

In this study, partial substitution of wheat flour with chickpea flour at the levels of 10, 20 and 30% was carried out to study their rheological and baking performance. Chickpea flour addition increased the water absorption and dough development time (p < 0.05), while, the extensibility of dough and the resistance to deformation were reduced. Regarding dough stability, it appears that 10% chickpea exhibited higher stability and resistance to mechanical mixing values than the control, while it decreased as the substitute level increases from 20% to 30%. The dough surface of the wheat dough and the blend with 10% was classified as “normal”, however the blend with 20% and 30% produced “sticky” dough surface. The presence of chickpea flour in dough affected bread quality in terms of volume, internal structure and texture. The color of crust and crumb got progressively darker as the level of chickpea flour substitution increased. While the substitution of wheat flour with 10% chickpea flour gave loaves as similar as control.