罗氏沼虾繁殖习性的研究

本文对罗氏沼虾的繁殖习性进行了较为详细地描述。性成熟的雌虾必须经过生殖蜕皮方可交配、产卵；受精卵粘附于腹肢刚毛上；在水温２４℃左右的条件下，幼体孵出约需２７天；性成熟雌虾能多次产卵。     

One novel mutation and copy number variation of NF1 gene in Chinese patients with type 1 neurofibromatosis

AIM: To investigate the mutations and the copy number variation of neurofibromatosis 1(NF1) gene in 2 sporadic patients with type 1 neurofibromatosis in China. METHODS: All coding exons and exon-intron boundaries of NF1 were amplified by PCR. The PCR products were sequenced. The DNA samples from 50 normal subjects were also sequenced for control. Multiplex ligation-dependent probe amplification (MLPA) was also employed to detect the copy number variation of NF1 gene in these patients. Long range PCR was used for the identification of the breakpoint in the large deletion of the gene. RESULTS: The novel mutation, c. 6345_6346 ins G (p. Leu2116Alafs*4), was detected in patient S736. This mutation was absent in her parents and the controls, indicating a de novo mutation. It caused open reading frame shifting, introducing a premature stop codon and resulting in the truncation of the 721 amino acids at the C terminus of the wild-type protein. This truncation cut off part of the armadillo (ARM)-type fold domain in the wild-type protein. A 1.3~1.9 Mb deletion of the gene was also detected in the other patient S743. The deletion spanned the whole NF1 gene and part of the flanking regions in both ends, but the breaking point was still unknown. CONCLUSION: We have identified a novel mutation of NF1, c.6345_6346 ins G (p.Leu2116Alafs*4). We also first report the copy number variation of NF1 gene in Chinese patients. The investigation will be helpful for the molecular diagnosis and understanding the pathogenesis of the disease.     

非酒精性脂肪肝病的研究进展

\t\t\t\t\t目的\t\t\t\t\t酸茶为德昂族独特的茶叶制品。探究酸茶对防治外源性膳食诱导引起的非酒精性脂肪肝的预防作用。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t通过动物试验,以保肝药为对照,分别灌胃不同剂量的酸茶水浸提物,为期4周。对血脂指标、肝功能指标、抗氧化指标以及胰岛素样生长因子-1 (IGF-1)和细胞色素P4502E1 (CYP2E1)的表达量进行检测,对试验大鼠肝脏组织进行病理性观察及评价。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t酸茶能够有效降低非酒精性脂肪肝试验大鼠血清中CHOL和TG的含量,同时增加HDL-C的水平;可降低大鼠血清中AST和ALT活性;升高大鼠血清和肝脏组织GSH-Px和SOD的活性。高剂量酸茶能够改善由于脂质堆积引起的肝组织病变问题。酸茶可提高试验大鼠肝脏组织中IGF-1 mRNA的表达,抑制CYP2E1 mRNA的表达。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结论\t\t\t\t\t德昂族酸茶具有预防大鼠非酒精性脂肪肝的作用。\t\t\t\t     

Exploiting generic platform technologies for the detection and identification of plant pathogens

The detection and identification of plant pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic skills to modern molecular-based methods. The wide range of methods employed reflects the great diversity of plant pathogens and the hosts they infect. The well-documented decline in taxonomic expertise, along with the need to develop ever more rapid and sensitive diagnostic methods has provided an impetus to develop technologies that are both generic and able to complement traditional skills and techniques. Real-time polymerase chain reaction (PCR) is emerging as one such generic platform technology and one that is well suited to high-throughput detection of a limited number of known target pathogens. Real-time PCR is now exploited as a front line diagnostic screening tool in human health, animal health, homeland security, biosecurity as well as plant health. Progress with developing generic techniques for plant pathogen identification, particularly of unknown samples, has been less rapid. Diagnostic microarrays and direct nucleic acid sequencing (de novo sequencing) both have potential as generic methods for the identification of unknown plant pathogens but are unlikely to be suitable as high-throughput detection techniques. This paper will review the application of generic technologies in the routine laboratory as well as highlighting some new techniques and the trend towards multi-disciplinary studies.     

黑荆树优树子代测定

本项试验采用5个优良家系子代,以瑞安市一般生产用种子为对照,随机区组设计。结果表明,优树梅16、梅14具有较好的遗传基因。1.主要性状:与对照比较,树高分别增加29.3％和20.7％,胸径增大54.0％和45.2％,树皮增厚24.1％和20.8％,单宁含量提高7.37％和6.61％;2.遗传增益:树高25.67％、胸径38.27％、树皮厚度15.62％、单宁含量10.57％。     

沿海防护林木麻黄病虫害综合控制技术研究报告——木麻黄抗病、抗虫优良品系筛选

本研究筛选出适宜于广东、福建沿海不同区域推广种植的Ａ１３、平２０－４等木麻黄优良抗病无性系;筛选出Ｃ３４、Ｃ３８、Ｃ３９、Ｃ４３、Ｃ４４、Ｃ４５等６个抗星天牛优良木麻黄品系;调查表明,Ａ１３等１０余个木麻黄无性系较普通木麻黄具有抗青枯病危害的作用。为沿海防护林木麻黄病虫害的综合控制提供科学依据。     

甘蓝型油菜抗菌核病材料的筛选研究

为了创造新的抗菌核病油菜种质材料,以品质优良、再生频率较高的甘蓝型黄籽油菜品系SH21,K81和甘蓝型黑籽品系P98的种子、预培养的下胚轴为诱变材料进行不同剂量的γ射线照射处理,以草酸为筛选剂,筛选抗(耐)菌核病材料,结果表明,草酸筛选浓度为700 mg/L,效果较好;3个品系通过草酸筛选,获得14株耐草酸植株,经过菌核病菌丝接种鉴定与筛选,筛选出7株抗性较高的植株。     

德昂族地区农村发展状况研究——以三台山德昂族乡为例

德昂族是一个人口较少民族,其生产和生活的地区是全国为数不多的“直过区”。三台山乡是全国唯一的德昂族乡。通过对三台山乡社会经济状况的调查,分析和评价德昂族地区的发展水平,从基础设施、人力资本、生产结构等方面分析该民族地区社会经济发展滞后的原因,并针对性地提出促进该地区社会经济发展的对策与建议。     

海南油楠叶的化学成分

[目的]明确海南油楠叶的化学成分。[方法]采用硅胶柱色谱对海南油楠叶的正己烷提取物和醋酸乙酯提取物进行分离,根据理化性质及波谱分析确定结构。[结果]分离得到9个化合物,鉴定为木栓酮(1)、3β-3-壬氧基-12-齐墩果烯(2)、8R,9R-环氧-β-石竹烯(3)、蒲公英萜醇(4)、豆甾烷-5烯-3-醇(5)、麦角甾-5-烯-3-醇(6)、2α,3β,-二羟基-24-去甲基-4(23)-12-齐墩果烯(7)、齐墩果酸(8)和邻苯二甲酸二戊酯(9)。[结论]化合物1~9均为首次从该植物的树叶中分离得到。     

浆茶杀青工艺及贮藏保鲜研究

本试验对浆茶杀青工艺及贮放保鲜进行了研究。结果表明 ,投叶量 10 0g ,微波功率 110 0w ,微波杀青 16 0s ,可有效钝化酶活性 ,并有较好的保绿效果 ;在pH 7 5,Vit.C 0 45mg/g贮放条件下利于浆茶保绿 ;添加柠檬酸可明显提高茶多酚儿茶素及叶绿素在贮放中的残留量 ,尤其对叶绿素的保留效果突出